Cell-type specific features of circular RNA expression.
TLDR
Using an improved computational approach for circular RNA identification, widespread circular RNA expression is found in Drosophila melanogaster and it is estimated that in humans, circular RNA may account for 1% as many molecules as poly(A) RNA.Abstract:
Thousands of loci in the human and mouse genomes give rise to circular RNA transcripts; at many of these loci, the predominant RNA isoform is a circle. Using an improved computational approach for circular RNA identification, we found widespread circular RNA expression in Drosophila melanogaster and estimate that in humans, circular RNA may account for 1% as many molecules as poly(A) RNA. Analysis of data from the ENCODE consortium revealed that the repertoire of genes expressing circular RNA, the ratio of circular to linear transcripts for each gene, and even the pattern of splice isoforms of circular RNAs from each gene were cell-type specific. These results suggest that biogenesis of circular RNA is an integral, conserved, and regulated feature of the gene expression program.read more
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Practical guide for circular RNA analysis: Steps, tips, and resources
TL;DR: In this paper, the authors provide guidelines to consider when developing studies on circRNAs, including detecting and selecting the circRNA, identifying their binding partners and sites of interaction, modulating circRNA levels, assessing copy numbers and stoichiometry, and addressing other points unique to circRNA analysis.
Journal ArticleDOI
Decoding epigenetic cell signaling in neuronal differentiation.
Mariana S. Vieira,Vânia Aparecida Mendes Goulart,Ricardo Parreira,Ricardo Parreira,Onésia Cristina Oliveira-Lima,Talita Glaser,Yahaira Naaldijk,Alejandra I. Ferrer,Vibha Harindra Savanur,Paola Alicea Reyes,Oleta A. Sandiford,Pranela Rameshwar,Henning Ulrich,Mauro Cunha Xavier Pinto,Mauro Cunha Xavier Pinto,Rodrigo R. Resende +15 more
TL;DR: The complexity of transcription factors, non-coding RNAs, and extracellular vesicles that are responsible for guiding and coordinating neural development are discussed.
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Expression Profile Screening and Bioinformatics Analysis of circRNA, LncRNA, and mRNA in Acute Myeloid Leukemia Drug-Resistant Cells
Meiling Li,Fuxue Meng,Quanyi Lu +2 more
TL;DR: This study provides a systematic outlook on the potential function of ncRNA in the molecular mechanisms of resistant AML cells and suggests Hsa-circ-0000978 and hsa- circ-0000483 might serve as potential prognostic biomarkers and therapeutic targets of AML resistance.
Journal ArticleDOI
circIQCH sponges miR-145 to promote breast cancer progression by upregulating DNMT3A expression.
Yuehua Li,Baohong Jiang,Zhengxi He,Hongbo Zhu,Rongfang He,Shanji Fan,Xiaoping Wu,Liming Xie,Xiusheng He +8 more
TL;DR: The pivotal role of circIQCH-miR-145-DNMT3A axis in breast cancer growth and metastasis via the mechanism of competing endogenous RNAs is demonstrated andcircIQCH could be a potential therapeutic target for breast cancer.
Journal ArticleDOI
Beyond Back Splicing, a Still Poorly Explored World: Non-Canonical Circular RNAs.
TL;DR: An unprecedented overview of all other (non-canonical) circRNAs that do not originate from the junction of two exons and to characterize their common properties, which all derive from non-coding sequences.
References
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Circular RNAs are a large class of animal RNAs with regulatory potency
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TL;DR: It is found that a human circRNA, antisense to the cerebellar degeneration-related protein 1 transcript (CDR1as), is densely bound by microRNA (miRNA) effector complexes and harbours 63 conserved binding sites for the ancient miRNA miR-7.
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Natural RNA circles function as efficient microRNA sponges
Thomas B. Hansen,Trine I. Jensen,Bettina Hjelm Clausen,Jesper B. Bramsen,Bente Finsen,Christian Kroun Damgaard,Jørgen Kjems +6 more
TL;DR: This study serves as the first functional analysis of a naturally expressed circular RNA, ciRS-7, which contains more than 70 selectively conserved miRNA target sites, and is highly and widely associated with Argonaute proteins in a miR-7-dependent manner.
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Circular RNAs are abundant, conserved, and associated with ALU repeats
William R. Jeck,Jessica A. Sorrentino,Kai Wang,Michael K. Slevin,Christin E. Burd,Jinze Liu,William F. Marzluff,Norman E. Sharpless +7 more
TL;DR: High-throughput sequencing of libraries prepared from ribosome-depleted RNA with or without digestion with the RNA exonuclease showed that ecircRNAs are abundant, stable, conserved and nonrandom products of RNA splicing that could be involved in control of gene expression.