Journal ArticleDOI
Characterization of variation and quantitative trait loci related to terpenoid indole alkaloid yield in a recombinant inbred line mapping population of Catharanthus roseus.
TLDR
The QTL were validated for use in marker assisted selection by the recombinant inbred line which transgressively expressed 16 traits contributory to the yield vinblastine, vindoline and catharanthine from leaves and roots that possessed favourable alleles of 13 relevant QTL.Abstract:
Improved Catharanthus roseus cultivars are required for high yields of vinblastine, vindoline and catharanthine and/or serpentine and ajmalicine, the pharmaceutical terpenoid indole alkaloids. An approach to derive them is to map QTL for terpenoid indole alkaloids yields, identify DNA markers tightly linked to the QTL and apply marker assisted selection. Towards the end, 197 recombinant inbred lines from a cross were grown over two seasons to characterize variability for seven biomass and 23 terpenoid indole alkaloids content-traits and yield-traits. The recombinant inbred lines were genotyped for 178 DNA markers which formed a framework genetic map of eight linkage groups (LG), spanning 1786.5 cM, with 10.0 cM average intermarker distance. Estimates of correlations between traits allowed selection of seven relatively more important traits for terpenoid indole alkaloids yields. QTL analysis was performed on them using single marker (regression) analysis, simple interval mapping and composite interval mapping procedures. A total of 20 QTL were detected on five of eight LG, 10 for five traits on LG1, five for four traits on LG2, three for one trait on LG3 and one each for different traits on LG three and four. QTL for the same or different traits were found clustered on three LG. Co-location of two QTL for biomass traits was in accord of correlation between them. The QTL were validated for use in marker assisted selection by the recombinant inbred line which transgressively expressed 16 traits contributory to the yield vinblastine, vindoline and catharanthine from leaves and roots that possessed favourable alleles of 13 relevant QTL.read more
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The journal of genetics
TL;DR: This is the oldest English language journal in genetics and retains its traditional interest in evolutionary research that is of relevance to geneticists, even if not explicitly genetic in nature.
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Empirical threshold values for quantitative trait mapping.
TL;DR: In this paper, an empirical method based on the concept of permutation test is proposed for estimating threshold values that are tailored to the experimental data at hand, which is demonstrated using two real data sets derived from F(2) and recombinant inbred plant populations.
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Phytochemical genomics of the Madagascar periwinkle: Unravelling the last twists of the alkaloid engine.
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Transcriptome sequencing of sea cucumber (Apostichopus japonicus) and the identification of gene-associated markers.
Z. C. Zhou,Y. Dong,H. J. Sun,A. F. Yang,Z. Chen,S. Gao,J. W. Jiang,X. Y. Guan,B. Jiang,Bo Wang +9 more
TL;DR: This project aimed to identify large numbers of gene‐associated markers and differentially expressed genes (DEGs) after lipopolysaccharides challenge in A. japonicus using high‐throughput transcriptome sequencing to provide abundant resources for genetic, evolutionary and ecological studies in sea cucumber.
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Economics of genomic selection: the role of prediction accuracy and relative genotyping costs
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References
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Journal ArticleDOI
Empirical threshold values for quantitative trait mapping.
TL;DR: An empirical method is described, based on the concept of a permutation test, for estimating threshold values that are tailored to the experimental data at hand, and is demonstrated using two real data sets derived from F(2) and recombinant inbred plant populations.
Journal ArticleDOI
Permutation Tests for Multiple Loci Affecting a Quantitative Character
TL;DR: Two extensions of the permutation-based method for estimating empirical threshold values are presented, which yield critical values that can be used to construct tests for the presence of minor QTL effects while accounting for effects of known major QTL.
Journal ArticleDOI
Chemistry and biology of monoterpene indole alkaloid biosynthesis
TL;DR: The biosynthetic pathways for several representative terpene indole alkaloids are described in detail, showing a diverse array of structures and biological activities.
Journal ArticleDOI
The use of molecular genetics in the improvement of agricultural populations.
TL;DR: The genes and genetic markers that are being discovered can be used to enhance the genetic improvement of breeding stock through marker-assisted selection.