Ciliostasis and loss of cilia induced by Mycoplasma hyopneumoniae in porcine tracheal organ cultures.
M C DeBey,Richard F. Ross +1 more
TLDR
In vivo- and in vitro-grown Mycoplasma hyopneumoniae organisms were inoculated onto newborn piglet tracheal organ cultures to provide a model for interaction of this organism with ciliated respiratory epithelium.Abstract:
In vivo- and in vitro-grown Mycoplasma hyopneumoniae organisms were inoculated onto newborn piglet tracheal organ cultures to provide a model for interaction of this organism with ciliated respiratory epithelium. Ciliostasis and loss of cilia in tracheal rings were induced by M. hyopneumoniae grown in vivo and with low-passage cultures when grown in vitro. Levels of calmodulin or dehydrogenase enzymes in tracheal ring epithelium were not altered even though ciliostasis and loss of cilia induced by M. hyopneumoniae were extensive. The capacity for inducing epithelial damage diminished with in vitro passage of the organism. Attempts to induce higher-passage cultures to attach to cilia, cause ciliostasis, or cause ciliary damage by supplementation of mycoplasmal medium with porcine lung extract failed. Epithelial damage induced by M. hyopneumoniae in tracheal rings was averted by using porcine immune serum or by separating the organisms from ciliated epithelium with a 0.1-microns-pore-size membrane. Attachment, or at least close association, of M. hyopneumoniae to ciliated epithelium appeared to be necessary to induce ciliostasis and loss of cilia in this model.read more
Citations
More filters
Journal ArticleDOI
Molecular Biology and Pathogenicity of Mycoplasmas
TL;DR: There is now solid genetic support for the hypothesis that mycoplasmas have evolved as a branch of gram-positive bacteria by a process of reductive evolution and developed various genetic systems providing a highly plastic set of variable surface proteins to evade the host immune system.
Journal ArticleDOI
Interaction of Mycoplasmas With Host Cells
TL;DR: Present knowledge is collated on the strategies employed by mycoplasmas while interacting with their host eukaryotic cells to demonstrate an impressive capability of maintaining a dynamic surface architecture that is antigenically and functionally versatile.
Journal ArticleDOI
Swine and Poultry Pathogens: the Complete Genome Sequences of Two Strains of Mycoplasma hyopneumoniae and a Strain of Mycoplasma synoviae
Ana Tereza Ribeiro de Vasconcelos,Henrique Bunselmeyer Ferreira,Cristiano Valim Bizarro,Sandro L. Bonatto,Marcos Oliveira de Carvalho,Paulo Marcos Pinto,Darcy F. de Almeida,Luiz Gonzaga Paula de Almeida,Almeida Rosana De,Leonardo Alves-Filho,Enedina Nogueira de Assunção,Vasco Azevedo,Maurício Reis Bogo,Marcelo M. Brigido,Marcelo Brocchi,Marcelo Brocchi,Hélio Almeida Burity,Anamaria A. Camargo,Sandro da Silva Camargo,Marta S. P. Carepo,Dirce Maria Carraro,J.C.M. Cascardo,Luiza Amaral de Castro,Gisele Cavalcanti,Gustavo Chemale,Rosane G. Collevatti,Cristina W. Cunha,Bruno Dallagiovanna,Bibiana Paula Dambrós,Odir Antônio Dellagostin,Clarissa Falcão,Fabiana Fantinatti-Garboggini,Maria Sueli Soares Felipe,Laurimar Fiorentin,Glória Regina Franco,Nara Suzy Aguiar De Freitas,Diego Frias,Thalles B. Grangeiro,Edmundo C. Grisard,Claudia Teixeira Guimarães,Mariangela Hungria,Silvia Neto Jardim,Marco Aurélio Krieger,Jomar Pereira Laurino,Lucymara Fassarella Agnez Lima,Maryellen I. Lopes,Élgion Lúcio da Silva Loreto,Humberto Maciel França Madeira,Gilson P. Manfio,Andrea Queiroz Maranhão,Christyanne T. Martinkovics,Silvia Regina Batistuzzo de Medeiros,Miguel Angêlo Martins Moreira,Márcia Neiva,Cicero Eduardo Ramalho-Neto,Marisa Fabiana Nicolás,Sergio C. Oliveira,Roger Ferreira Cury Paixão,Fábio O. Pedrosa,Sérgio D.J. Pena,Maristela Pereira,Lilian Pereira-Ferrari,Itamar Antônio Piffer,Luciano da Silva Pinto,Deise Porto Potrich,Anna Christina M. Salim,Fabrício R. Santos,Renata Schmitt,Maria Paula Cruz Schneider,Augusto Schrank,Irene Silveira Schrank,Adriana F. Schuck,Héctor N. Seuánez,Denise Wanderlei Silva,Rosane Silva,Sergio Ceroni da Silva,Célia Maria de Almeida Soares,Kelly Rose Lobo de Souza,Rangel C. Souza,Charley Christian Staats,Maria B. R. Steffens,Santuza M. R. Teixeira,Turán P. Ürményi,Marilene Henning Vainstein,Luciana W. Zuccherato,Andrew J. G. Simpson,Arnaldo Zaha +86 more
TL;DR: Genomic comparisons revealed that reduction in genome size implied loss of redundant metabolic pathways, with maintenance of alternative routes in different species, and indicated a likely transfer event of hemagglutinin-coding DNA sequences from M. gallisepticum to M. synoviae.
Journal ArticleDOI
Polymicrobial respiratory disease in pigs
TL;DR: This review discusses the latest findings on polymicrobial respiratory disease in pigs and recommends best practices for control of swine respiratory disease outbreaks caused by concurrent infection of two or more pathogens.
Journal ArticleDOI
Influenza virus infection decreases tracheal mucociliary velocity and clearance of Streptococcus pneumoniae.
TL;DR: Using a mouse model of influenza infection followed by S. pneumoniae infection, it is found that an influenza infection does not increase the number of pneumococci initially present within the trachea, but does inhibit pneumococcal clearance by 2 hours after infection.
References
More filters
Journal ArticleDOI
The ability of Salmonella to enter mammalian cells is affected by bacterial growth state.
Catherine A. Lee,Stanley Falkow +1 more
TL;DR: It is speculated that oxygen limitation might be an environmental cue that triggers the expression of Salmonella invasiveness within the intestinal lumen and other tissues.
Journal ArticleDOI
Deletions of chromosomal regions coding for fimbriae and hemolysins occur in vitro and in vivo in various extraintestinal Escherichia coli isolates.
TL;DR: It is concluded that excisions of 'pathogenicity islands' from chromosomes of pathogenic E. coli strains are not restricted to the laboratory but also occur in vivo and may represent a general mechanism of bacterial virulence modulation.
Journal ArticleDOI
Sequences required for expression of Bordetella pertussis virulence factors share homology with prokaryotic signal transduction proteins.
Beatrice Aricò,Jeff F. Miller,Craig R. Roy,Scott Stibitz,Denise M. Monack,Stanley Falkow,Roy Gross,Rino Rappuoli +7 more
TL;DR: A model in which BvgB and the N-terminal portion of BvgC are localized in the periplasm is proposed, in which the predicted protein products of bvgA and bvgC share homology with a family of prokaryotic regulatory proteins that respond to environmental stimuli and are members of two-component sensory transduction systems.
Journal Article
Some recommendations concerning primary isolation of Mycoplasma suipneumoniae and Mycoplasma flocculare a survey.
TL;DR: A description is given of a new medium with which primary isolation of mycoplasma species of the porcine respiratory tract is usually successful, and various additives often recommended for myCoplasma cultivation have been examined for growth promoting effect.
Journal ArticleDOI
Epithelial cell surfaces induce Salmonella proteins required for bacterial adherence and invasion.
TL;DR: Stable adherence to and invasion of epithelial cells by S. choleraesuis and S. typhimurium were found to require de novo synthesis of several new bacterial proteins required for Salmonella virulence, which appears to be a coordinately regulated system dependent on trypsin- and neuraminidase-sensitive structures present on the epithelial cell surface.