Open AccessJournal Article
Some recommendations concerning primary isolation of Mycoplasma suipneumoniae and Mycoplasma flocculare a survey.
TLDR
A description is given of a new medium with which primary isolation of mycoplasma species of the porcine respiratory tract is usually successful, and various additives often recommended for myCoplasma cultivation have been examined for growth promoting effect.Abstract:
Two mycoplasma species of the porcine respiratory tract: Mycoplasma suipneumoniae and Mycoplasma flocculare, have been notoriously difficult to cultivate. In the present paper a description is given of a new medium with which primary isolation of these organisms is usually successful. The basal medium is made from commercial dehydrated products. Pig serum is added and phenol red used as pH indicator. Contrary to what is customary in the preparation of mycoplasma media, various inorganic salts (Hank's balanced salt solution) are included and penicillin-G replaced as a bacteriostatic by bacitracin and meticillin. The volume of water is adjusted so as to give isotonia. Various additives often recommended for mycoplasma cultivation have been examined for growth promoting effect; apparently, however, they were all without effect. Working procedures for primary isolation trials are briefly described.read more
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Ciliostasis and loss of cilia induced by Mycoplasma hyopneumoniae in porcine tracheal organ cultures.
M C DeBey,Richard F. Ross +1 more
TL;DR: In vivo- and in vitro-grown Mycoplasma hyopneumoniae organisms were inoculated onto newborn piglet tracheal organ cultures to provide a model for interaction of this organism with ciliated respiratory epithelium.
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Evaluation of local and systemic immune responses induced by intramuscular injection of a Mycoplasma hyopneumoniae bacterin to pigs.
TL;DR: Vaccination against M hyopneumoniae induced local, mucosal, humoral, and cellular immune responses, and reduced the severity of lung lesions in challenged pigs, suggesting that mucosal antibodies, mediation of the inflammatory response, and cell-mediated immune responses are important for control of mycoplasmal pneumonia in pigs.
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Mycoplasma hyopneumoniae infection in pigs; duration of the disease and evaluation of four diagnostic assays
Vibeke Sørensen,Peter Ahrens,Kristen Barfod,Anne Avlund Feenstra,Niels Christian Feld,N.F. Friis,Vivi Bille-Hansen,Niels Jensen,Michael W. Pedersen +8 more
TL;DR: An evaluation of cultivation, immunofluorescence, ELISA and polymerase chain reaction for demonstration of M. hyopneumoniae in lungs showed that all four methods have a high sensitivity in the acute stages of pneumonia.
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Application of a nested polymerase chain reaction assay to detect Mycoplasma hyopneumoniae from nasal swabs.
TL;DR: A nested PCR using 2 species-specific sets of primers from the 16S ribosomal DNA gave positive results with as little as 80 microorganisms and did not cross-react with other mycoplasma species or with other microorganisms commonly found in the respiratory tract of pigs.
Journal ArticleDOI
Identification and characterization of a Mycoplasma hyopneumoniae adhesin.
TL;DR: Results indicate that P97 functions as an adhesin of M. hyopneumoniae, suggesting that antigenic variation of adhesins may be a pathogenic mechanism utilized by M.hyopneumoniane to evade the porcine immune system.
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