CircSETD3 (Hsa_circ_0000567) acts as a sponge for microRNA-421 inhibiting hepatocellular carcinoma growth
Liangliang Xu,Xinfu Feng,Xiangyong Hao,Peng Wang,Yanfang Zhang,Xiaobo Zheng,Lian Li,Shengsheng Ren,Ming Zhang,Ming-Qing Xu +9 more
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TLDR
CircSETD3 is a novel tumour suppressor of HCC and is a valuable prognostic biomarker that acts as a sponge for miR-421 and verified that mitogen-activated protein kinase (MAPK)14 is a Novel target of miR -421.Abstract:
Circular RNAs (circRNAs) play important roles in tumourigenesis and tumour progression. However, the expression profiles and functions of circRNAs in hepatocellular carcinoma (HCC) are largely unclear. The expression profiles of circRNAs in HCC were identified through microarray analysis and were validated through quantitative reverse transcription polymerase chain reaction (qRT-PCR). Survival curves were plotted using the Kaplan-Meier method and compared using the log-rank test. The circular structure of candidate circRNA was confirmed through Sanger sequencing, divergent primer PCR, and RNase R treatments. Proliferation of HCC cells was evaluated in vitro and in vivo. The microRNA (miRNA) sponge mechanism of circRNAs was demonstrated using dual-luciferase reporter and RNA immunoprecipitation assays. CircSETD3 (hsa_circRNA_0000567/hsa_circRNA_101436) was significantly downregulated in HCC tissues and cell lines. Low expression of circSETD3 in HCC tissues significantly predicted an unfavourable prognosis and was correlated with larger tumour size and poor differentiation of HCC in patients. In vitro experiments showed that circSETD3 inhibited the proliferation of HCC cells and induced G1/S arrest in HCC cells. In vivo studies revealed that circSETD3 was stably overexpressed in a xenograft mouse model and inhibited the growth of HCC. Furthermore, we demonstrated that circSETD3 acts as a sponge for miR-421 and verified that mitogen-activated protein kinase (MAPK)14 is a novel target of miR-421. CircSETD3 is a novel tumour suppressor of HCC and is a valuable prognostic biomarker. Moreover, circSETD3 inhibits the growth of HCC partly through the circSETD3/miR-421/MAPK14 pathway.read more
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A Novel Circular RNA, circ_0005394, Predicts Unfavorable Prognosis and Contributes to Hepatocellular Carcinoma Progression by Regulating miR-507/E2F3 and miR-515-5p/CXCL6 Signaling Pathways
TL;DR: High expression of circ_0005394 was associated with larger tumor size, more advanced TNM stages, and poorer overall survival for the patients with HCC, providing a novel perspective for clarifying its pathogenesis.
Journal ArticleDOI
hsa_circ_0006916 promotes hepatocellular carcinoma progression by activating the miR-337-3p/STAT3 axis.
TL;DR: The results suggested that hsa_circ_0006916 acted as a sponge of miR-337-3p and had an important functional use in the regulation of STAT3 levels in hepatocellular carcinoma cells.
Journal ArticleDOI
TCEB3 is Regulated by Circ-0000212/miR-140-3p Axis to Promote the Progression of Cervical Cancer.
TL;DR: In this article, the role of TCEB3 in cervical cancer progression was explored, and it was shown that TCEb3 attenuated cell proliferation and invasion and promoted apoptosis of cervical cancer cells.
Journal ArticleDOI
Circular RNA circSETD3 hampers cell growth, migration, and stem cell properties in bladder cancer through sponging miR-641 to upregulate PTEN.
TL;DR: In this paper, the role of a novel circular RNA circSETD3 in the development of urothelial cancer was investigated. But it was not shown that circRNAs involved in regulating BLCA progression.
Journal ArticleDOI
Circular RNA CircEPB41L2 Functions as Tumor Suppressor in Hepatocellular Carcinoma Through Sponging miR-590-5p
Feng Chen,Lei He,Liman Qiu,Liman Qiu,Yang Zhou,Yang Zhou,Zhenli Li,Zhenli Li,Geng Chen,Geng Chen,Fuli Xin,Fuli Xin,Xiuqing Dong,Xiuqing Dong,Haipo Xu,Haipo Xu,Gaoxiong Wang,Jingfeng Liu,Jingfeng Liu,Zhixiong Cai,Zhixiong Cai +20 more
TL;DR: In this paper, the potential function and molecular mechanism of circEPB41L2 (hsa_circ_0077837) during hepatocellular carcinoma (HCC) progression was investigated.
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