CircSETD3 (Hsa_circ_0000567) acts as a sponge for microRNA-421 inhibiting hepatocellular carcinoma growth
Liangliang Xu,Xinfu Feng,Xiangyong Hao,Peng Wang,Yanfang Zhang,Xiaobo Zheng,Lian Li,Shengsheng Ren,Ming Zhang,Ming-Qing Xu +9 more
Reads0
Chats0
TLDR
CircSETD3 is a novel tumour suppressor of HCC and is a valuable prognostic biomarker that acts as a sponge for miR-421 and verified that mitogen-activated protein kinase (MAPK)14 is a Novel target of miR -421.Abstract:
Circular RNAs (circRNAs) play important roles in tumourigenesis and tumour progression. However, the expression profiles and functions of circRNAs in hepatocellular carcinoma (HCC) are largely unclear. The expression profiles of circRNAs in HCC were identified through microarray analysis and were validated through quantitative reverse transcription polymerase chain reaction (qRT-PCR). Survival curves were plotted using the Kaplan-Meier method and compared using the log-rank test. The circular structure of candidate circRNA was confirmed through Sanger sequencing, divergent primer PCR, and RNase R treatments. Proliferation of HCC cells was evaluated in vitro and in vivo. The microRNA (miRNA) sponge mechanism of circRNAs was demonstrated using dual-luciferase reporter and RNA immunoprecipitation assays. CircSETD3 (hsa_circRNA_0000567/hsa_circRNA_101436) was significantly downregulated in HCC tissues and cell lines. Low expression of circSETD3 in HCC tissues significantly predicted an unfavourable prognosis and was correlated with larger tumour size and poor differentiation of HCC in patients. In vitro experiments showed that circSETD3 inhibited the proliferation of HCC cells and induced G1/S arrest in HCC cells. In vivo studies revealed that circSETD3 was stably overexpressed in a xenograft mouse model and inhibited the growth of HCC. Furthermore, we demonstrated that circSETD3 acts as a sponge for miR-421 and verified that mitogen-activated protein kinase (MAPK)14 is a novel target of miR-421. CircSETD3 is a novel tumour suppressor of HCC and is a valuable prognostic biomarker. Moreover, circSETD3 inhibits the growth of HCC partly through the circSETD3/miR-421/MAPK14 pathway.read more
Citations
More filters
Journal ArticleDOI
Epigenetics in hepatocellular carcinoma
TL;DR: In this article , the authors have summarized the epigenetic modifications involved in HCC progression and highlighted the potential biomarkers for diagnosis and drug development, which can provide a meaningful improvement in overall survival and prediction of therapeutic outcomes.
Journal ArticleDOI
The circRNA circIFI30 promotes progression of triple-negative breast cancer and correlates with prognosis
Lei Xing,Rui Yang,Xiaosong Wang,Xiaying Zheng,Xin Yang,Luyu Zhang,Rong Jiang,Guosheng Ren,Junxia Chen +8 more
TL;DR: The mechanism by which circ IFI30 could promote TNBC progression through circIFI30/miR-520b-3p/CD44 axis is uncovers and it is found that circIFi30 was significantly up-regulated in TNBC tissues and cells using quantitative real-time PCR and in situ hybridization.
Journal ArticleDOI
Exosomal hsa_circ_0004658 derived from RBPJ overexpressed-macrophages inhibits hepatocellular carcinoma progression via miR-499b-5p/JAM3
TL;DR: In this article , the role of exosomes derived from RBPJ-overexpressed macrophages (RBPJ+/+ Mφ-Exo) in hepatocellular carcinoma (HCC) was assessed using circRNA microarray.
Journal ArticleDOI
The circular RNA circMAST1 promotes hepatocellular carcinoma cell proliferation and migration by sponging miR-1299 and regulating CTNND1 expression
Xiufeng Yu,Ping Sheng,Jing Sun,Xijuang Zhao,Junting Zhang,Yiying Li,YiMeng Zhang,Wenxiu Zhang,Jianqi Wang,Kunpeng Liu,Daling Zhu,Hongchi Jiang +11 more
TL;DR: It is found that circMAST1 may serve as a novel biomarker for HCC and elicits HCC progression by sponging miRNA-1299 and stabilizing CTNND1 and sponged miR- 1299 to stabilize the expression of CTNNd1 and promoted tumorigenic features in HCC cell lines.
Journal ArticleDOI
The SRSF1/circATP5B/miR-185-5p/HOXB5 feedback loop regulates the proliferation of glioma stem cells via the IL6-mediated JAK2/STAT3 signaling pathway
Junshuang Zhao,Yang Jiang,Yang Jiang,Haiying Zhang,Jinpeng Zhou,Lian Chen,Hao Li,Jinkun Xu,Guoqing Zhang,Zhitao Jing +9 more
TL;DR: This study identified the SRSF1/circATP5B/miR-185-5p/HOXB5 feedback loop in GSCs, which provides an effective biomarker for glioma diagnosis and prognostic evaluation.
References
More filters
Journal ArticleDOI
Global cancer statistics
TL;DR: A substantial proportion of the worldwide burden of cancer could be prevented through the application of existing cancer control knowledge and by implementing programs for tobacco control, vaccination, and early detection and treatment, as well as public health campaigns promoting physical activity and a healthier dietary intake.
Journal ArticleDOI
Global cancer statistics, 2012
Lindsey A. Torre,Freddie Bray,Rebecca L. Siegel,Jacques Ferlay,Joannie Lortet-Tieulent,Ahmedin Jemal +5 more
TL;DR: A substantial portion of cancer cases and deaths could be prevented by broadly applying effective prevention measures, such as tobacco control, vaccination, and the use of early detection tests.
Journal ArticleDOI
EASL-EORTC clinical practice guidelines : management of hepatocellular carcinoma
Peter R. Galle,Alejandro Forner,Josep M. Llovet,Vincenzo Mazzaferro,Fabio Piscaglia,Jean-Luc Raoul,Peter Schirmacher,Valérie Vilgrain +7 more
TL;DR: The following Clinical Practice Guidelines will give up-to-date advice for the clinical management of patients with hepatocellular carcinoma, as well as providing an in-depth review of all the relevant data leading to the conclusions herein.
Journal ArticleDOI
Circular RNAs are a large class of animal RNAs with regulatory potency
Sebastian Memczak,Marvin Jens,Antigoni Elefsinioti,Francesca Torti,Janna Krueger,Agnieszka Rybak,Luisa Maier,Sebastian D. Mackowiak,Lea H. Gregersen,Mathias Munschauer,Alexander Loewer,Ulrike Ziebold,Markus Landthaler,Christine Kocks,Ferdinand le Noble,Nikolaus Rajewsky +15 more
TL;DR: It is found that a human circRNA, antisense to the cerebellar degeneration-related protein 1 transcript (CDR1as), is densely bound by microRNA (miRNA) effector complexes and harbours 63 conserved binding sites for the ancient miRNA miR-7.
Journal ArticleDOI
Natural RNA circles function as efficient microRNA sponges
Thomas B. Hansen,Trine I. Jensen,Bettina Hjelm Clausen,Jesper B. Bramsen,Bente Finsen,Christian Kroun Damgaard,Jørgen Kjems +6 more
TL;DR: This study serves as the first functional analysis of a naturally expressed circular RNA, ciRS-7, which contains more than 70 selectively conserved miRNA target sites, and is highly and widely associated with Argonaute proteins in a miR-7-dependent manner.