Journal ArticleDOI
Cloning and expression of the mouse pgk-1 gene and the nucleotide sequence of its promoter.
TLDR
A number of conserved motifs in the promoter may indicate a significant role for these sequences in expression of the pgk-1 gene, which is contained within a 16-kb region of the X chromosome.About:
This article is published in Gene.The article was published on 1987-01-01. It has received 356 citations till now. The article focuses on the topics: TATA box & Promoter.read more
Citations
More filters
Journal ArticleDOI
Targeted mutation of the DNA methyltransferase gene results in embryonic lethality.
TL;DR: Results indicate that while a 3-fold reduction in levels of genomic m5C has no detectable effect on the viability or proliferation of ES cells in culture, a similar reduction of DNA methylation in embryos causes abnormal development and embryonic lethality.
Patent
Transgenic non-human animals capable of producing heterologous antibodies
Nils Lonberg,Robert M. Kay +1 more
TL;DR: In this paper, a transgenic non-human animals capable of producing heterologous antibodies and methods for producing human sequence antibodies which bind to human antigens with substantial affinity are described.
Journal ArticleDOI
RAG-1-deficient mice have no mature B and T lymphocytes
Peter Mombaerts,John Iacomini,Randall S. Johnson,Karl Herrup,Susumu Tonegawa,Virginia E. Papaioannou +5 more
TL;DR: The introduction of a mutation in RAG-1 into the germline of mice via gene targeting in embryonic stem cells is described and it is shown that this mutation either activates or catalyzes the V(D)J recombination reaction of immunoglobulin and T cell receptor genes.
Journal ArticleDOI
Targeted disruption of the c-src proto-oncogene leads to osteopetrosis in mice
TL;DR: It is demonstrated that src is not required for general cell viability (possibly because of functional overlap with other tyrosine kinases related to src) and an essential role for src in bone formation is uncovered.
Journal ArticleDOI
Visualizing an Olfactory Sensory Map
Peter Mombaerts,Fan Wang,Catherine Dulac,Steve K. Chao,Adriana Nemes,Monica Mendelsohn,James Edmondson,Richard Axel +7 more
TL;DR: A genetic approach is developed to visualize axons from olfactory sensory neurons expressing a given odorant receptor, as they project to the Olfactory bulb, which provides direct support for a model in which a topographic map of receptor activation encodes odor quality in the ofactory bulb.
References
More filters
Book
Molecular Cloning: A Laboratory Manual
TL;DR: Molecular Cloning has served as the foundation of technical expertise in labs worldwide for 30 years as mentioned in this paper and has been so popular, or so influential, that no other manual has been more widely used and influential.
Journal ArticleDOI
DNA sequencing with chain-terminating inhibitors
TL;DR: A new method for determining nucleotide sequences in DNA is described, which makes use of the 2',3'-dideoxy and arabinon nucleoside analogues of the normal deoxynucleoside triphosphates, which act as specific chain-terminating inhibitors of DNA polymerase.
Journal ArticleDOI
A rapid alkaline extraction procedure for screening recombinant plasmid DNA
H C Birnboim,J Doly +1 more
TL;DR: In this paper, a procedure for extracting plasmid DNA from bacterial cells is described, which is simple enough to permit the analysis by gel electrophoresis of 100 or more clones per day, yet yields DNA which is pure enough to be digestible by restriction enzymes.
Arapid alkaline extraction procedure forscreening recombinant plasmid DNA
TL;DR: The method is simple enough to permit the analysis by gel electrophoresis of 100 or more clones per day yet yields plasmid DNA which is pure enough to be digestible by restriction enzymes, and achievesequate pH control without using a pH meter.
Journal ArticleDOI
A new technique for the assay of infectivity of human adenovirus 5 DNA.
Frank L. Graham,A. J. Van Der Eb +1 more
TL;DR: A new technique for assaying infectivity of adenovirus 5 DNA has been developed and a reproducible relationship between amounts of DNA inoculated per culture and numbers of plaques produced was demonstrated.
Related Papers (5)
Disruption of the proto-oncogene int-2 in mouse embryo-derived stem cells: a general strategy for targeting mutations to non-selectable genes
Site-directed mutagenesis by gene targeting in mouse embryo-derived stem cells.
Kirk R. Thomas,Mario R. Capecchi +1 more