Journal ArticleDOI
Comparison of covalent and noncovalent labeling with near-infrared dyes for the high-performance liquid chromatographic determination of human serum albumin
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TLDR
Noncovalent and covalent methods of labeling protein with near-infrared polymethine cyanine dyes were compared for use in analyzing human serum albumin by high-performance liquid chromatography (HPLC) withNearinfrared absorbance detection.Abstract:
Noncovalent and covalent methods of labeling protein with near-infrared polymethine cyanine dyes were compared for use in analyzing human serum albumin (HSA) by high-performance liquid chromatography (HPLC) with near-infrared absorbance detection. While noncovalent labeling was faster than covalent labeling and took place in the physiological pH range, covalent labeling was more stable under conditions encountered in many of the widely used types of HPLC. Covalently labeled HSA protein peaks indicated uniform labeling of amino groups at both hydrophilic and hydrophobic binding sites, while noncovalent labeling showed a preference for hydrophobic binding sites.read more
Citations
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Journal ArticleDOI
Synthesis and In Vivo Fate of Zwitterionic Near-Infrared Fluorophores
Hak Soo Choi,Khaled Nasr,Sergey Alyabyev,Dina Feith,Jeong Heon Lee,Soon Hee Kim,Soon Hee Kim,Yoshitomo Ashitate,Hoon Hyun,Gabor Patonay,Lucjan Strekowski,Maged Henary,John V. Frangioni +12 more
TL;DR: A longstanding problem in the field of image-guided surgery is the development of ideal near-infrared (NIR) fluorophores, and several classes of novel molecules have been described but none to date exhibit simultaneous low background binding, bifunctionality, excellent optical properties, low protein binding, and high serum stability.
Journal ArticleDOI
Organic alternatives to quantum dots for intraoperative near-infrared fluorescent sentinel lymph node mapping.
Shunsuke Ohnishi,Stephen Johnson Lomnes,Rita G. Laurence,Andrew Gogbashian,Giuliano Mariani,John V. Frangioni +5 more
TL;DR: A family of 800 nm organic heptamethine indocyanine-based contrast agents for SLN mapping spanning a spectrum from 775 Da small molecules to 7 MDa nanocolloids are described and one contrast agent emerged as the molecule with the best overall performance with respect to entry to lymphatics, flow to the SLN, retention in theSLN, fluorescence yield and reproducibility.
Journal ArticleDOI
“Vapochromic” Compounds as Environmental Sensors. 2. Synthesis and Near-Infrared and Infrared Spectroscopy Studies of [Pt(arylisocyanide)4][Pt(CN)4] upon Exposure to Volatile Organic Compound Vapors
Charles A. Daws,Charles A. Daws,Christopher L. Exstrom,Christopher L. Exstrom,John R. Sowa,John R. Sowa,Kent R. Mann +6 more
TL;DR: In this article, the synthesis, characterization, and vis−NIR−IR vapochromic/spectroscopic studies are reported for isocyanide compounds of the form [Pt(arylisocyanides)4][Pt (CN)4] (where arylisocide = p-CNC6H4CnH2n+1; n = 1, 6, 10, 12, 14).
Journal ArticleDOI
Noncovalent labeling of biomolecules with red and near- infrared dyes.
TL;DR: The applications of luminescent cyanine and squarylium dyes are reviewed and the main advantage of noncovalent labeling is that it affects the functional activity of the biomolecule to a lesser extent.
Journal ArticleDOI
Continuous separation of high molecular weight compounds using a microliter volume free-flow electrophoresis microstructure.
TL;DR: Study of the resolution of bradykinin and ribonuclease A as a function of field strength at various sample and carrier flow rates indicated that, for maximum throughput, high field strengths and high flow rates were required, however, no optimal conditions were found.
References
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Journal ArticleDOI
Solvophobic interactions in liquid chromatography with nonpolar stationary phases
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Journal ArticleDOI
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TL;DR: The three-dimensional structure of human serum albumin has been solved at 6.0 angstrom (A) resolution by the method of multiple isomorphous replacement and revealed the structure as a predominantly alpha-helical globin protein in which the course of the polypeptide can be traced.
Journal ArticleDOI
Molecular structure of the human albumin gene is revealed by nucleotide sequence within q11-22 of chromosome 4.
Phillip P. Minghetti,Duane E. Ruffner,Wun-Jing Kuang,O. E. Dennison,J. W. Hawkins,W. G. Beattie,Achilles Dugaiczyk +6 more
TL;DR: The human albumin gene spans 16,961 nucleotides from the putative "Cap" site to the first poly(A) addition site and appears to have been recently invaded by Alu repetitive sequences.
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Immunologic Specificity and Molecular Structure1
TL;DR: This chapter formulates the basic concepts for understanding immunologic specificity and stresses the probable importance of apolar interactions in the antigen-antibody reactions.
Journal ArticleDOI
Binding of bilirubin to human serum albumin - determination of the dissociation constants.
TL;DR: The binding of bilirubin to serum albumin has been known for more than fifty years, but the affinity is high and it has not been possible to determine the dissociation constant, since a sensitive method for measuring the very low concentrations of non-bound bilirUBin has not be available.
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