Conversion of adult pancreatic α-cells to β-cells after extreme β-cell loss
Fabrizio Thorel,Virginie Nepote,Isabelle Avril,Kenji Kohno,Renaud Desgraz,Simona Chera,Pedro Luis Herrera +6 more
TLDR
In this article, a transgenic model of diphtheria-toxin-induced acute selective near-total beta-cell ablation was used to investigate whether adult mammals can differentiate (regenerate) new beta-cells after extreme, total β-cell loss, as in diabetes.Abstract:
Pancreatic insulin-producing beta-cells have a long lifespan, such that in healthy conditions they replicate little during a lifetime. Nevertheless, they show increased self-duplication after increased metabolic demand or after injury (that is, beta-cell loss). It is not known whether adult mammals can differentiate (regenerate) new beta-cells after extreme, total beta-cell loss, as in diabetes. This would indicate differentiation from precursors or another heterologous (non-beta-cell) source. Here we show beta-cell regeneration in a transgenic model of diphtheria-toxin-induced acute selective near-total beta-cell ablation. If given insulin, the mice survived and showed beta-cell mass augmentation with time. Lineage-tracing to label the glucagon-producing alpha-cells before beta-cell ablation tracked large fractions of regenerated beta-cells as deriving from alpha-cells, revealing a previously disregarded degree of pancreatic cell plasticity. Such inter-endocrine spontaneous adult cell conversion could be harnessed towards methods of producing beta-cells for diabetes therapies, either in differentiation settings in vitro or in induced regeneration.read more
Citations
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Chitosan-coated rectangular DNA nanospheres for better outcomes of anti-diabetic drug
Mirza Muhammad Faran Ashraf Baig,Mirza Muhammad Faran Ashraf Baig,Muhammad Naveed,Muhammad Abbas,Said Abasse Kassim,Ghulam Jilany Khan,Ghulam Jilany Khan,Sana Ullah,Muhammad Sohail,Waqas Nawaz,Muhammad Rizwan Younis,Muhammad Tayyab Ansari +11 more
TL;DR: It is concluded that the nanospheres efficiently bypassed the gastric acidity improving glycemic control in Db-Db/mouse model and did not cause damage to pancreas associated with the sustained and prolonged release of VL.
Journal ArticleDOI
Activin Enhances α- to β-Cell Transdifferentiation as a Source For β-Cells In Male FSTL3 Knockout Mice.
TL;DR: The results suggest that α- to β-cell transdifferentiation is influenced by activin signaling and may contribute substantially to α-cell mass, while activin treatment of isolated islets significantly increased the number of YFP+/Ins+.
Journal ArticleDOI
Transplantation of betatrophin-expressing adipose-derived mesenchymal stem cells induces β-cell proliferation in diabetic mice.
Liang-liang Sun,Tian-Jin Liu,Limei Li,Wei Tang,Jun-jie Zou,Xiangfang Chen,Jiaoyang Zheng,Bei-Ge Jiang,Yong-quan Shi +8 more
TL;DR: ADMSCs-BET may replace insulin injections by increasing the number of endogenous insulin-producing cells in patients with diabetes by influencing islet proliferation, β-cell specific transcription factor expression, and islet production of insulin under the stimulation of glucose or KCl and Arg.
Journal ArticleDOI
Progenitor cells and TNF-alpha involvement during morphological changes in pancreatic islets of obese mice.
Alessandra Alves Thole,Alessandra Cordeiro de Souza Rodrigues-Cunha,Simone Nunes de Carvalho,Erica Patricia Garcia-Souza,Erika Cortez,Ana Carolina Stumbo,Laís de Carvalho,Anibal Sanchez Moura +7 more
TL;DR: The results showed that islet hypertrophy is established in obese groups at day 28 and remained until adulthood, and TNF-α expression in pancreatic islets was increased in both young and adult obese groups when compared to control groups.
Journal ArticleDOI
Current status of regeneration of pancreatic β-cells
Kohtaro Minami,Susumu Seino +1 more
TL;DR: Establishing a protocol to generate fully functional insulin‐secreting cells that closely resemble native β‐cells is a critical matter in regenerative medicine for diabetes.
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Adult pancreatic beta-cells are formed by self-duplication rather than stem-cell differentiation.
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Related Papers (5)
Adult pancreatic beta-cells are formed by self-duplication rather than stem-cell differentiation.
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