Open AccessJournal Article
Correction for Longitudinal shift in diabetic wound microbiota correlates with prolonged skin defense response
Elizabeth A. Grice,Evan S. Snitkin,Laura J. Yockey,Dustin M. Bermudez,Kenneth W. Liechty,Julia A. Segre +5 more
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TLDR
In this article, a longitudinal selective shift in wound microbiota coincides with impaired wound healing in diabetic mice (Leprdb/db; db/db) and a parallel shift in longitudinal gene expression that occurs in a cluster of genes related to the immune response.Abstract:
Diabetics frequently suffer from chronic, nonhealing wounds. Although bacterial colonization and/or infection are generally acknowledged to negatively impact wound healing, the precise relationship between the microbial community and impaired wound healing remains unclear. Because the host cutaneous defense response is proposed to play a key role in modulating microbial colonization, we longitudinally examined the diabetic wound microbiome in tandem with host tissue gene expression. By sequencing 16S ribosomal RNA genes, we show that a longitudinal selective shift in wound microbiota coincides with impaired healing in diabetic mice (Leprdb/db; db/db). We demonstrate a parallel shift in longitudinal gene expression that occurs in a cluster of genes related to the immune response. Further, we establish a correlation between relative abundance of Staphylococcus spp. and the expression of cutaneous defense response genes. Our data demonstrate that integrating two types of global datasets lends a better understanding to the dynamics governing host–microbe interactions.read more
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The human microbiome: at the interface of health and disease
Ilseung Cho,Martin J. Blaser +1 more
TL;DR: The large-scale dynamics of the microbiome can be described by many of the tools and observations used in the study of population ecology, andiphering the metagenome and its aggregate genetic information can also be used to understand the functional properties of the microbial community.
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The skin microbiome
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Wound repair and regeneration: Mechanisms, signaling, and translation
TL;DR: In this review, emerging concepts in tissue regeneration and repair are highlighted, and some perspectives on how to translate current knowledge into viable clinical approaches for treating patients with wound-healing pathologies are provided.
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Wound Healing: A Cellular Perspective.
TL;DR: It is shown that changes in the microenvironment including alterations in mechanical forces, oxygen levels, chemokines, extracellular matrix and growth factor synthesis directly impact cellular recruitment and activation, leading to impaired states of wound healing.
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Epithelialization in Wound Healing: A Comprehensive Review
Irena Pastar,Olivera Stojadinovic,Natalie C. Yin,Horacio Ramirez,Aron G. Nusbaum,Andrew P. Sawaya,Shailee Patel,Laiqua Khalid,R. Isseroff,Marjana Tomic-Canic +9 more
TL;DR: The pivotal role of keratinocytes in epithelialization is focused on, including cellular processes and mechanisms of their regulation during re-epithelialization, and their cross talk with other cell types participating in wound healing.
References
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Gene set enrichment analysis: A knowledge-based approach for interpreting genome-wide expression profiles
Aravind Subramanian,Pablo Tamayo,Vamsi K. Mootha,Sayan Mukherjee,Benjamin L. Ebert,Michael A. Gillette,Amanda G. Paulovich,Scott L. Pomeroy,Todd R. Golub,Eric S. Lander,Jill P. Mesirov +10 more
TL;DR: The Gene Set Enrichment Analysis (GSEA) method as discussed by the authors focuses on gene sets, that is, groups of genes that share common biological function, chromosomal location, or regulation.
Journal ArticleDOI
The human microbiome: at the interface of health and disease
Ilseung Cho,Martin J. Blaser +1 more
TL;DR: The large-scale dynamics of the microbiome can be described by many of the tools and observations used in the study of population ecology, andiphering the metagenome and its aggregate genetic information can also be used to understand the functional properties of the microbial community.
Journal ArticleDOI
The skin microbiome
TL;DR: An enhanced understanding of the skin microbiome is necessary to gain insight into microbial involvement in human skin disorders and to enable novel promicrobial and antimicrobial therapeutic approaches for their treatment.
Journal ArticleDOI
Topographical and temporal diversity of the human skin microbiome.
Elizabeth A. Grice,Heidi H. Kong,Sean Conlan,Clayton Deming,Joie Davis,Alice C. Young,Nisc Comparative Sequencing Program,Gerard G. Bouffard,Robert W. Blakesley,Patrick R. Murray,Eric D. Green,Maria L. Turner,Julia A. Segre +12 more
TL;DR: This topographical and temporal survey of body sites from 10 healthy human individuals sampled over time provides a baseline for studies that examine the role of bacterial communities in disease states and the microbial interdependencies required to maintain healthy skin.
Journal ArticleDOI
Evidence that the diabetes gene encodes the leptin receptor: identification of a mutation in the leptin receptor gene in db/db mice.
Hong Chen,Olga Charlat,Louis A. Tartaglia,Elizabeth A. Woolf,Xun Weng,Stephen J Ellis,Nathan D Lakey,Janice A. Culpepper,Karen J More,Roger E. Breitbart,Geoffrey M. Duyk,Robert I. Tepper,Jay P. Morgenstern +12 more
TL;DR: It is predicted that the long intrACEllular domain form of OB-R is crucial for initiating intracellular signal transduction, and as a corollary, the inability to produce this form ofOB-R leads to the severe obese phenotype found in db/db mice.