Journal ArticleDOI
Correlation between acetaldehyde and ethanol resistance and expression of HSP genes in yeast strains isolated during the biological aging of sherry wines.
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TLDR
Gen expression analysis revealed induction of the heat shock protein (HSP) genes HSP12, HSP82, and especially HSP26 and HSP104, under acetaldehyde stress in most of the strains.Abstract:
In the production of sherry wines, the process of biological aging is essential for the development of their organoleptic properties. This process involves velum formation by "flor" yeasts. Several of these yeast strains have been isolated and characterized with regard to their genetic, physiological and metabolic properties. In this work, we studied their resistance to cold-, osmotic-, oxidative-, ethanol- and acetaldehyde-stress, and found, in most cases, a correlation between resistance to acetaldehyde stress and ethanol stress and isolation from "soleras." Moreover, gene expression analysis revealed induction of the heat shock protein (HSP) genes HSP12, HSP82, and especially HSP26 and HSP104, under acetaldehyde stress in most of the strains. In strain C, there was a clear correlation between resistance to ethanol and acetaldehyde, the high induction of HSP genes by these compounds and its presence as the predominant strain in most levels of several soleras.read more
Citations
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Journal ArticleDOI
Aldehyde Dehydrogenases in Cellular Responses to Oxidative/electrophilic Stress
Surendra Singh,Chad Brocker,Vindhya Koppaka,Ying Chen,Brian C. Jackson,Akiko Matsumoto,David C. Thompson,Vasilis Vasiliou +7 more
TL;DR: The ALDH superfamily represents a fundamentally important class of enzymes that contributes significantly to the management of electrophilic/oxidative stress within living systems and is associated with a variety of pathological conditions in humans.
Journal ArticleDOI
Adaptive evolution of wine yeast.
Amparo Querol,M. Teresa Fernández-Espinar,Marcel·lí del Olmo,Marcel·lí del Olmo,Eladio Barrio +4 more
TL;DR: In this review, the different mechanisms involved in the adaptive evolution of wine yeast strains are described.
Journal ArticleDOI
Winemaking Biochemistry and Microbiology: Current Knowledge and Future Trends
TL;DR: An updated overview on the main biochemical and microbiological contributions of the different techniques that can be used in winemaking is provided and the production of special wines, such as sparkling wines, ‘sur lie’ wines, and biologically aged wines are also studied.
Journal ArticleDOI
Yeast Genome-Wide Expression Analysis Identifies a Strong Ergosterol and Oxidative Stress Response during the Initial Stages of an Industrial Lager Fermentation
TL;DR: Genome-wide expression analysis was successful in identifying environmental conditions that are important in industrial yeast fermentation and showed that erg mutants producing altered sterols were highly sensitive to oxidative stress-generating compounds.
Journal ArticleDOI
Response to acetaldehyde stress in the yeast Saccharomyces cerevisiae involves a strain-dependent regulation of several ALD genes and is mediated by the general stress response pathway
TL;DR: Under several growth conditions, further addition of acetaldehyde or ethanol in flor yeasts induced the expression of some ALD genes and led to an increase in ALDH activity, which is consistent with their need to obtain energy from ethanol during biological ageing processes.
References
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Journal ArticleDOI
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Deval A. Lashkari,Joseph L. DeRisi,John H. McCusker,Allen F. Namath,Cristl Gentile,Seung Y. Hwang,Patrick O. Brown,Ronald W. Davis +7 more
TL;DR: High-density DNA microarrays of yeast ORFs can monitor hybridization to ORFs for applications such as quantitative differential gene expression analysis and screening for sequence polymorphisms at the whole genome level.