Creation of a Bacterial Cell Controlled by a Chemically Synthesized Genome
Daniel G. Gibson,John I. Glass,Carole Lartigue,Vladimir N. Noskov,Ray-Yuan Chuang,Mikkel A. Algire,Gwynedd A. Benders,Michael G. Montague,Li Ma,Monzia Moodie,Chuck Merryman,Sanjay Vashee,Radha Krishnakumar,Nacyra Assad-Garcia,Cynthia Andrews-Pfannkoch,Evgeniya A. Denisova,Lei Young,Zhi-Qing Qi,Thomas H. Segall-Shapiro,Christopher H. Calvey,Prashanth P. Parmar,Clyde A. Hutchison,Hamilton O. Smith,J. Craig Venter +23 more
Reads0
Chats0
TLDR
The design, synthesis, and assembly of the 1.08–mega–base pair Mycoplasma mycoides JCVI-syn1.0 genome starting from digitized genome sequence information and its transplantation into a M. capricolum recipient cell to create new cells that are controlled only by the synthetic chromosome are reported.Abstract:
We report the design, synthesis, and assembly of the 1.08-mega-base pair Mycoplasma mycoides JCVI-syn1.0 genome starting from digitized genome sequence information and its transplantation into a M. capricolum recipient cell to create new M. mycoides cells that are controlled only by the synthetic chromosome. The only DNA in the cells is the designed synthetic DNA sequence, including "watermark" sequences and other designed gene deletions and polymorphisms, and mutations acquired during the building process. The new cells have expected phenotypic properties and are capable of continuous self-replication.read more
Citations
More filters
Journal ArticleDOI
Engineering in complex systems
Matthias Bujara,Sven Panke +1 more
TL;DR: Recent progress in dynamic in vivo analysis of metabolism and conceptual advances in orthogonalizing cells should enhance the reliability of engineering designs in the future and allow a more rational design of metabolic systems.
Journal ArticleDOI
Building genomes to understand biology.
TL;DR: Different classes of genetic manipulation that are enabled by synthetic genomics, as well as biological problems they each can help solve, are discussed.
Journal ArticleDOI
Building the blueprint of life.
TL;DR: How comparative genomes, gene essentiality data, naturally small genomes, and metabolic modeling are all being applied to produce a catalogue of the biological functions essential for life are discussed.
Journal ArticleDOI
A synthetic biology approach to bio-chem-ICT: first moves towards chemical communication between synthetic and natural cells
Giordano Rampioni,Fabio Mavelli,Luisa Damiano,Francesca D'Angelo,Marco Messina,Livia Leoni,Pasquale Stano +6 more
TL;DR: Novel aspects of the impact that synthetic biology can express in a field traditionally based on computer science: information and communication technologies (ICTs), an area that will consider taking into account also possible implications for artificial intelligence (AI) research.
References
More filters
疟原虫var基因转换速率变化导致抗原变异[英]／Paul H, Robert P, Christodoulou Z, et al//Proc Natl Acad Sci U S A
TL;DR: PfPMP1）与感染红细胞、树突状组胞以及胎盘的单个或多个受体作用，在黏附及免疫逃避中起关键的作�ly.
Journal ArticleDOI
Enzymatic assembly of DNA molecules up to several hundred kilobases
Daniel G. Gibson,Lei Young,Ray-Yuan Chuang,J. Craig Venter,Clyde A. Hutchison,Hamilton O. Smith +5 more
TL;DR: An isothermal, single-reaction method for assembling multiple overlapping DNA molecules by the concerted action of a 5′ exonuclease, a DNA polymerase and a DNA ligase is described.
Journal ArticleDOI
Whole-genome random sequencing and assembly of Haemophilus influenzae Rd.
Fleischmann Rd,Adams,Owen White,Rebecca A. Clayton,Ewen F. Kirkness,Anthony R. Kerlavage,Carol J. Bult,J F Tomb,Brian Dougherty,Merrick Jm +9 more
TL;DR: An approach for genome analysis based on sequencing and assembly of unselected pieces of DNA from the whole chromosome has been applied to obtain the complete nucleotide sequence of the genome from the bacterium Haemophilus influenzae Rd.
“Bioinformatics” 특집을 내면서
TL;DR: Assessment of medical technology in the context of commercialization with Bioentrepreneur course, which addresses many issues unique to biomedical products.
Journal ArticleDOI
Nucleotide sequence of bacteriophage G4 DNA.
TL;DR: The sequence identifies many of the features responsible for the production of the proteins of the nine known genes of the organism, including initiation and termination sites for the proteins and RNAs.
Related Papers (5)
Complete chemical synthesis, assembly, and cloning of a Mycoplasma genitalium genome.
Daniel G. Gibson,Gwynedd A. Benders,Cynthia Andrews-Pfannkoch,Evgeniya A. Denisova,Holly Baden-Tillson,Jayshree Zaveri,Timothy B. Stockwell,Anushka Brownley,David W. Thomas,Mikkel A. Algire,Chuck Merryman,Lei Young,Vladimir N. Noskov,John I. Glass,J. Craig Venter,Clyde A. Hutchison,Hamilton O. Smith +16 more
Design and synthesis of a minimal bacterial genome
Clyde A. Hutchison,Ray-Yuan Chuang,Vladimir N. Noskov,Nacyra Assad-Garcia,Thomas J. Deerinck,Mark H. Ellisman,John Gill,Krishna Kannan,Bogumil J. Karas,Li Ma,James F. Pelletier,Zhi Qing Qi,R. Alexander Richter,Elizabeth A. Strychalski,Lijie Sun,Yo Suzuki,Billyana Tsvetanova,Kim S. Wise,Hamilton O. Smith,John I. Glass,Chuck Merryman,Daniel G. Gibson,J. Craig Venter +22 more