Creation of a Bacterial Cell Controlled by a Chemically Synthesized Genome
Daniel G. Gibson,John I. Glass,Carole Lartigue,Vladimir N. Noskov,Ray-Yuan Chuang,Mikkel A. Algire,Gwynedd A. Benders,Michael G. Montague,Li Ma,Monzia Moodie,Chuck Merryman,Sanjay Vashee,Radha Krishnakumar,Nacyra Assad-Garcia,Cynthia Andrews-Pfannkoch,Evgeniya A. Denisova,Lei Young,Zhi-Qing Qi,Thomas H. Segall-Shapiro,Christopher H. Calvey,Prashanth P. Parmar,Clyde A. Hutchison,Hamilton O. Smith,J. Craig Venter +23 more
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TLDR
The design, synthesis, and assembly of the 1.08–mega–base pair Mycoplasma mycoides JCVI-syn1.0 genome starting from digitized genome sequence information and its transplantation into a M. capricolum recipient cell to create new cells that are controlled only by the synthetic chromosome are reported.Abstract:
We report the design, synthesis, and assembly of the 1.08-mega-base pair Mycoplasma mycoides JCVI-syn1.0 genome starting from digitized genome sequence information and its transplantation into a M. capricolum recipient cell to create new M. mycoides cells that are controlled only by the synthetic chromosome. The only DNA in the cells is the designed synthetic DNA sequence, including "watermark" sequences and other designed gene deletions and polymorphisms, and mutations acquired during the building process. The new cells have expected phenotypic properties and are capable of continuous self-replication.read more
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Combining two genomes in one cell: stable cloning of the Synechocystis PCC6803 genome in the Bacillus subtilis 168 genome.
TL;DR: Given the significant diversity in genome structure observed upon horizontal DNA transfer in nature, this stable laboratory-generated composite genome raised fundamental questions concerning two complete genomes in one cell and may be generally applicable to other genomes or genome loci of free-living organisms.
Journal ArticleDOI
Cloning whole bacterial genomes in yeast
Gwynedd A. Benders,Vladimir N. Noskov,Evgeniya A. Denisova,Carole Lartigue,Daniel G. Gibson,Nacyra Assad-Garcia,Ray-Yuan Chuang,William Carrera,Monzia Moodie,Mikkel A. Algire,Quang Phan,Nina Alperovich,Sanjay Vashee,Chuck Merryman,J. Craig Venter,Hamilton O. Smith,John I. Glass,Clyde A. Hutchison +17 more
TL;DR: The cloning of whole bacterial genomes in the yeast Saccharomyces cerevisiae as single-DNA molecules is reported, and these genomes appear to be stably maintained in a host that has efficient, well-established methods for DNA manipulation.
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Synthetic Genomics | Options for Governance
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Multiple personal genomes await
TL;DR: The next challenge — linking human genetic variation with physiology and disease — will be as great as the one genomicists faced a decade ago, says J. Craig Venter.
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