Creation of a Bacterial Cell Controlled by a Chemically Synthesized Genome
Daniel G. Gibson,John I. Glass,Carole Lartigue,Vladimir N. Noskov,Ray-Yuan Chuang,Mikkel A. Algire,Gwynedd A. Benders,Michael G. Montague,Li Ma,Monzia Moodie,Chuck Merryman,Sanjay Vashee,Radha Krishnakumar,Nacyra Assad-Garcia,Cynthia Andrews-Pfannkoch,Evgeniya A. Denisova,Lei Young,Zhi-Qing Qi,Thomas H. Segall-Shapiro,Christopher H. Calvey,Prashanth P. Parmar,Clyde A. Hutchison,Hamilton O. Smith,J. Craig Venter +23 more
Reads0
Chats0
TLDR
The design, synthesis, and assembly of the 1.08–mega–base pair Mycoplasma mycoides JCVI-syn1.0 genome starting from digitized genome sequence information and its transplantation into a M. capricolum recipient cell to create new cells that are controlled only by the synthetic chromosome are reported.Abstract:
We report the design, synthesis, and assembly of the 1.08-mega-base pair Mycoplasma mycoides JCVI-syn1.0 genome starting from digitized genome sequence information and its transplantation into a M. capricolum recipient cell to create new M. mycoides cells that are controlled only by the synthetic chromosome. The only DNA in the cells is the designed synthetic DNA sequence, including "watermark" sequences and other designed gene deletions and polymorphisms, and mutations acquired during the building process. The new cells have expected phenotypic properties and are capable of continuous self-replication.read more
Citations
More filters
Journal ArticleDOI
Interfacial Effects of Superhydrophobic Plant Surfaces: A Review
TL;DR: In this paper, a review briefly introduces the physical-chemical basis of super-hydrophobic plant surfaces in nature to explain how the superhydrophobicity of plant surfaces can be applied to different biomimetic functional materials with relevance to technological applications.
Journal ArticleDOI
Bacterial microcompartments moving into a synthetic biological world
TL;DR: The molecular structure of bacterial microcompartments is examined and the potential to engineer these intriguing organelles for biotechnological applications is explored.
Journal ArticleDOI
Application of synthetic biology for production of chemicals in yeast Saccharomyces cerevisiae
Mingji Li,Irina Borodina +1 more
TL;DR: Computational tools for the prediction of biochemical pathways, molecular biology methods for assembly of DNA parts into pathways, and for introducing the pathways into the host, and finally approaches for optimizing performance of the introduced pathways are described.
Journal ArticleDOI
Essential genes in Bacillus subtilis: a re-evaluation after ten years.
TL;DR: Detailed analyses have revealed that 31 genes that were thought to be essential are in fact non-essential whereas 20 novel essential genes have been described, and 261 genes coding for 259 proteins and two functional RNAs are regarded essential as of January 2013.
Journal ArticleDOI
DNA storage: research landscape and future prospects
TL;DR: The fundamental theory, research history, and technical challenges of DNA storage are summarized, and the prospect of DNA, and organic polymers in general, as a novel class of data storage medium is evaluated.
References
More filters
疟原虫var基因转换速率变化导致抗原变异[英]／Paul H, Robert P, Christodoulou Z, et al//Proc Natl Acad Sci U S A
TL;DR: PfPMP1）与感染红细胞、树突状组胞以及胎盘的单个或多个受体作用，在黏附及免疫逃避中起关键的作�ly.
Journal ArticleDOI
Enzymatic assembly of DNA molecules up to several hundred kilobases
Daniel G. Gibson,Lei Young,Ray-Yuan Chuang,J. Craig Venter,Clyde A. Hutchison,Hamilton O. Smith +5 more
TL;DR: An isothermal, single-reaction method for assembling multiple overlapping DNA molecules by the concerted action of a 5′ exonuclease, a DNA polymerase and a DNA ligase is described.
Journal ArticleDOI
Whole-genome random sequencing and assembly of Haemophilus influenzae Rd.
Fleischmann Rd,Adams,Owen White,Rebecca A. Clayton,Ewen F. Kirkness,Anthony R. Kerlavage,Carol J. Bult,J F Tomb,Brian Dougherty,Merrick Jm +9 more
TL;DR: An approach for genome analysis based on sequencing and assembly of unselected pieces of DNA from the whole chromosome has been applied to obtain the complete nucleotide sequence of the genome from the bacterium Haemophilus influenzae Rd.
“Bioinformatics” 특집을 내면서
TL;DR: Assessment of medical technology in the context of commercialization with Bioentrepreneur course, which addresses many issues unique to biomedical products.
Journal ArticleDOI
Nucleotide sequence of bacteriophage G4 DNA.
TL;DR: The sequence identifies many of the features responsible for the production of the proteins of the nine known genes of the organism, including initiation and termination sites for the proteins and RNAs.
Related Papers (5)
Complete chemical synthesis, assembly, and cloning of a Mycoplasma genitalium genome.
Daniel G. Gibson,Gwynedd A. Benders,Cynthia Andrews-Pfannkoch,Evgeniya A. Denisova,Holly Baden-Tillson,Jayshree Zaveri,Timothy B. Stockwell,Anushka Brownley,David W. Thomas,Mikkel A. Algire,Chuck Merryman,Lei Young,Vladimir N. Noskov,John I. Glass,J. Craig Venter,Clyde A. Hutchison,Hamilton O. Smith +16 more
Design and synthesis of a minimal bacterial genome
Clyde A. Hutchison,Ray-Yuan Chuang,Vladimir N. Noskov,Nacyra Assad-Garcia,Thomas J. Deerinck,Mark H. Ellisman,John Gill,Krishna Kannan,Bogumil J. Karas,Li Ma,James F. Pelletier,Zhi Qing Qi,R. Alexander Richter,Elizabeth A. Strychalski,Lijie Sun,Yo Suzuki,Billyana Tsvetanova,Kim S. Wise,Hamilton O. Smith,John I. Glass,Chuck Merryman,Daniel G. Gibson,J. Craig Venter +22 more