Critical review on biofilm methods
Joana Azeredo,Nuno F. Azevedo,Romain Briandet,Nuno Cerca,Tom Coenye,Ana Rita Costa,Mickaël Desvaux,Giovanni Di Bonaventura,Michel Hébraud,Zoran Jaglic,Miroslava Kačániová,Susanne Knøchel,Anália Lourenço,Filipe Mergulhão,Rikke Louise Meyer,George Nychas,Manuel Simões,Odile Tresse,Claus Sternberg +18 more
TLDR
This review aims at helping scientists in finding the most appropriate and up-to-date methods to study their biofilms by giving a critical perspective, highlighting the advantages and limitations of several methods.Abstract:
Biofilms are widespread in nature and constitute an important strategy implemented by microorganisms to survive in sometimes harsh environmental conditions. They can be beneficial or have a negative impact particularly when formed in industrial settings or on medical devices. As such, research into the formation and elimination of biofilms is important for many disciplines. Several new methodologies have been recently developed for, or adapted to, biofilm studies that have contributed to deeper knowledge on biofilm physiology, structure and composition. In this review, traditional and cutting-edge methods to study biofilm biomass, viability, structure, composition and physiology are addressed. Moreover, as there is a lack of consensus among the diversity of techniques used to grow and study biofilms. This review intends to remedy this, by giving a critical perspective, highlighting the advantages and limitations of several methods. Accordingly, this review aims at helping scientists in finding the most appropriate and up-to-date methods to study their biofilms.read more
Citations
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Quantification of biofilm in microtiter plates: overview oftesting conditions and practical recommendations for assessmentof biofilm production by staphylococci.
Srdjan Stepanović,Dragana Vuković,Veronika Holá,Giovanni DiBonaventura,Slobodanka Djukic,Ivana Cirkovic,Filip Růžička +6 more
TL;DR: The details of all steps involved in the quantification of biofilm formation in microtiter plates are described in this paper, where the authors present a protocol incorporating information on assessment of Biofilm production by staphylococci, gained both by direct experience as well as by analysis of methods for assayingBiofilm production.
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Nanomaterials with a photothermal effect for antibacterial activities: an overview
Jing-Wei Xu,Ke Yao,Zhi-Kang Xu +2 more
TL;DR: Four functional categories of antibacterial nanomaterials with a photothermal conversion effect are classified: carbon-based nanoconjugates of graphene derivatives or carbon nanotubes, noble metal nanom material mainly from gold and silver, metallic compound nanocomposites, and polymeric as well as other nanostructures.
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Biofilm-related disease.
TL;DR: ABSTRACT Biofilm formation represents a protected mode of growth that renders bacterial cells less susceptible to antimicrobials and to killing by host immune effector mechanisms and s...
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A review of methods for the detection of pathogenic microorganisms
Piumie Rajapaksha,Aaron Elbourne,Sheeana Gangadoo,Robert Brown,Daniel Cozzolino,James Chapman +5 more
TL;DR: This review presents an opportunity to fill a knowledge gap for an extremely important research area; discussing the main techniques, biology, chemistry, miniaturisation, sensing and the emerging state-of-the-art research and developments for detection of pathogens in food, water, blood and faecal samples.
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Marine Plastic Debris: A New Surface for Microbial Colonization.
Robyn J. Wright,Gabriel Erni-Cassola,Vinko Zadjelovic,Mira Latva,Joseph Alexander Christie-Oleza,Joseph Alexander Christie-Oleza +5 more
TL;DR: It is concluded that research so far has not shown Plastisphere communities to starkly differ from microbial communities on other inert surfaces, which is particularly true for mature biofilm assemblages.
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