Journal ArticleDOI
Determinants of interferon-stimulated gene induction by RNAi vectors
Stephanie Pebernard,Richard Iggo +1 more
TLDR
To avoid interferon induction by U6 vectors, it is recommended to preserve the wild-type sequence around the transcription start site, in particular a C/G sequence at positions -1/+1, and a simple cloning strategy using the Gateway recombination system that facilitates this task is described.About:
This article is published in Differentiation.The article was published on 2004-03-01. It has received 170 citations till now. The article focuses on the topics: Small hairpin RNA & RNA interference.read more
Citations
More filters
Journal ArticleDOI
Unlocking the potential of the human genome with RNA interference
Gregory J. Hannon,John J. Rossi +1 more
TL;DR: RNAi has revolutionized approaches to decoding gene function and has the potential to be exploited therapeutically, and clinical trials to test this possibility are already being planned.
Journal ArticleDOI
Revealing the world of RNA interference
Craig C. Mello,Darryl Conte +1 more
TL;DR: The recent discoveries of RNA interference and related RNA silencing pathways have revolutionized the understanding of gene regulation and have potential as a therapeutic strategy to reduce the expression of problem genes.
Journal ArticleDOI
Noise amidst the silence: off-target effects of siRNAs?
TL;DR: These findings suggest that siRNAs can regulate the expression of unintended targets, and argue for further experiments on the mechanism and extent of off-target gene regulation(s).
Journal ArticleDOI
Inhibition of respiratory syncytial virus infection with intranasal siRNA nanoparticles targeting the viral NS1 gene.
Weidong Zhang,H. Yang,Xiaoyuan Kong,Subhra Mohapatra,Homero San Juan-Vergara,Gary Hellermann,Sumita Behera,Rajeswari Singam,Richard F. Lockey,Shyam S. Mohapatra +9 more
TL;DR: Mice treated intranasally with siNS1 nanoparticles before or after infection with RSV showed substantially decreased virus titers in the lung and decreased inflammation and airway reactivity compared to controls, suggesting siNS 1 nanoparticles may provide an effective inhibition of RSV infection in humans.
Journal ArticleDOI
The silent revolution: RNA interference as basic biology, research tool, and therapeutic.
TL;DR: RNAi may provide an important new therapeutic modality for treating infection, cancer, neurodegenerative disease, and other illnesses, although in vivo delivery of small interfering RNAs into cells remains a significant obstacle.
References
More filters
Journal ArticleDOI
Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans
Andrew Fire,SiQun Xu,Mary K. Montgomery,Steven A. Kostas,Steven A. Kostas,Samuel E. Driver,Craig C. Mello +6 more
TL;DR: To their surprise, it was found that double-stranded RNA was substantially more effective at producing interference than was either strand individually, arguing against stochiometric interference with endogenous mRNA and suggesting that there could be a catalytic or amplification component in the interference process.
Journal ArticleDOI
Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells
TL;DR: 21-nucleotide siRNA duplexes provide a new tool for studying gene function in mammalian cells and may eventually be used as gene-specific therapeutics.
Journal ArticleDOI
Recognition of double-stranded RNA and activation of NF-kappaB by Toll-like receptor 3.
TL;DR: It is shown that mammalian TLR3 recognizes dsRNA, and that activation of the receptor induces the activation of NF-κB and the production of type I interferons (IFNs).
Journal ArticleDOI
In Vivo Gene Delivery and Stable Transduction of Nondividing Cells by a Lentiviral Vector
Luigi Naldini,Ulrike Blömer,Philippe Gallay,Daniel S. Ory,Richard C. Mulligan,Fred H. Gage,Inder M. Verma,Didier Trono +7 more
TL;DR: The ability of HIV-based viral vectors to deliver genes in vivo into nondividing cells could increase the applicability of retroviral vectors in human gene therapy.
Journal ArticleDOI
A System for Stable Expression of Short Interfering RNAs in Mammalian Cells
TL;DR: It is shown that siRNA expression mediated by this vector causes efficient and specific down-regulation of gene expression, resulting in functional inactivation of the targeted genes.