Determination of Membrane Cholesterol Partition Coefficient Using a Lipid Vesicle–Cyclodextrin Binary System: Effect of Phospholipid Acyl Chain Unsaturation and Headgroup Composition
Shui-Lin Niu,Burton J. Litman +1 more
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TLDR
Large differences observed in cholesterol-lipid interactions contribute to the forces responsible for lateral domain formation in plasma membranes and may also be responsible for the heterogeneous cholesterol distribution in cellular membranes.About:
This article is published in Biophysical Journal.The article was published on 2002-12-01 and is currently open access. It has received 207 citations till now. The article focuses on the topics: Phospholipid & Biological membrane.read more
Citations
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Docosahexaenoic acid: membrane properties of a unique fatty acid.
TL;DR: DHA's interaction with other membrane lipids, particularly cholesterol, may play a prominent role in modulating the local structure and function of cell membranes.
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Ceramide Selectively Displaces Cholesterol from Ordered Lipid Domains (Rafts) IMPLICATIONS FOR LIPID RAFT STRUCTURE AND FUNCTION
Megha,Erwin London +1 more
TL;DR: It is proposed that ceramides and cholesterol compete for association with rafts because of a limited capacity of raft lipids with large headgroups to accommodate small headgroup lipids in a manner that prevents unfavorable contact between the hydrocarbon groups of the small head group lipids and the surrounding aqueous environment.
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Cholesterol–phospholipid interactions, the liquid-ordered phase and lipid rafts in model and biological membranes
TL;DR: The results of recent studies are reviewed, which in the view call into question the existence of lipid rafts in membranes, at least in the form commonly depicted.
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Intracellular cholesterol transport
TL;DR: This chapter provides an overview of the recent understanding of intracellular transport of cholesterol, aimed to create a link between the well characterized biophysical properties of cholesterol in model membranes and its behavior in living cells.
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Role of cholesterol in the function and organization of G-protein coupled receptors.
TL;DR: A novel mechanism by which membrane cholesterol could affect structure and function of GPCRs is proposed and it is envisaged that there could be specific/nonannular cholesterol binding site(s) in the serotonin(1A) receptor.
References
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Phosphorus Assay in Column Chromatography
TL;DR: If the highest accuracy was not required, the following manipulations simplified and speeded multiple total phosphorus determinations on the eluates from column chromatographic separations.
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Functional rafts in cell membranes
Kai Simons,Elina Ikonen +1 more
TL;DR: A new aspect of cell membrane structure is presented, based on the dynamic clustering of sphingolipids and cholesterol to form rafts that move within the fluid bilayer that function as platforms for the attachment of proteins when membranes are moved around inside the cell and during signal transduction.
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Enzymatic determination of total serum cholesterol.
TL;DR: An enzymatic method is described for determination of total serum cholesterol by use of a single aqueous reagent and has excellent precision.
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Structure and function of sphingolipid- and cholesterol-rich membrane rafts.
Deborah A. Brown,Erwin London +1 more
TL;DR: This work will review the evidence that rafts exist in cells and focus on their structure, or the organization of raft lipids and proteins, and the role of rafts in signaling in hematopoietic cells.
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Extraction of Cholesterol with Methyl-β-Cyclodextrin Perturbs Formation of Clathrin-coated Endocytic Vesicles
Siv Kjersti Rodal,Grethe Skretting,Øystein Garred,Frederik Vilhardt,Bo van Deurs,Kirsten Sandvig +5 more
TL;DR: The results indicate that although clathrin-independent (and caveolae-independent) endocytosis still operates after removal of cholesterol, cholesterol is essential for the formation of clathin-coated endocytic vesicles.
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Structure and function of sphingolipid- and cholesterol-rich membrane rafts.
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