Sphingomyelin/phosphatidylcholine/cholesterol phase diagram: boundaries and composition of lipid rafts
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TLDR
The diagrams here described are used to rationalize literature results, some of them apparently discrepant, and to discuss lipid rafts within the framework of liquid-ordered/liquid-disordered phase coexistence.About:
This article is published in Biophysical Journal.The article was published on 2003-10-01 and is currently open access. It has received 857 citations till now. The article focuses on the topics: Liquid ordered phase & POPC.read more
Citations
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Model systems, lipid rafts, and cell membranes.
Kai Simons,Winchil L.C. Vaz +1 more
TL;DR: This review critically analyzes what is known of phase behavior and liquid-liquid immiscibility in model systems and compares these data with what isknown of domain formation in cell membranes.
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Separation of liquid phases in giant vesicles of ternary mixtures of phospholipids and cholesterol.
Sarah L. Veatch,Sarah L. Keller +1 more
TL;DR: In this article, the authors use fluorescence microscopy to directly observe liquid phases in giant unilamellar vesicles and find a simple relationship between chain melting temperature and miscibility transition temperature that holds for both phosphatidylcholine and sphingomyelin lipids.
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Lipid rafts: contentious only from simplistic standpoints
TL;DR: An updated model of lipid rafts is proposed that readily accommodates diverse views on plasma-membrane micro-organization and helps clarify the role of cholesterol in this complex, non-random organization.
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Seeing spots: Complex phase behavior in simple membranes
Sarah L. Veatch,Sarah L. Keller +1 more
TL;DR: The rich phase behavior observed in binary and ternary systems is described, including miscibility behavior varies with lipid type, lipid ratio, lipid oxidation, and level of impurity.
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Miscibility phase diagrams of giant vesicles containing sphingomyelin.
Sarah L. Veatch,Sarah L. Keller +1 more
TL;DR: Fluorescence microscopy is used to observe coexisting liquid domains in vesicles containing SM, an unsaturated phosphatidylcholine lipid (either DOPC or POPC), and cholesterol, and detail indirect evidence for a three-phase coexistence of one solid and two liquid phases.
References
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Book
Principles of fluorescence spectroscopy
TL;DR: This book describes the fundamental aspects of fluorescence, the biochemical applications of this methodology, and the instrumentation used in fluorescence spectroscopy.
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Functional rafts in cell membranes
Kai Simons,Elina Ikonen +1 more
TL;DR: A new aspect of cell membrane structure is presented, based on the dynamic clustering of sphingolipids and cholesterol to form rafts that move within the fluid bilayer that function as platforms for the attachment of proteins when membranes are moved around inside the cell and during signal transduction.
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Lipid rafts reconstituted in model membranes.
Christian Dietrich,Luis A. Bagatolli,Zoya Volovyk,Nancy L. Thompson,Moshe Levi,Ken Jacobson,Enrico Gratton +6 more
TL;DR: The notion that in biomembranes selected lipids could laterally aggregate to form more ordered, detergent-resistant lipid rafts into which glycosphingolipid- and cholesterol-rich lipid domains partition is strongly supported by this study.
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A role for lipid shells in targeting proteins to caveolae, rafts, and other lipid domains.
TL;DR: This work proposes that the molecular address for proteins targeted to lipid domains is a lipid shell, which is high in cholesterol and sphingolipids, have a light buoyant density, and function in both endocytosis and cell signaling.
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Phase equilibria of cholesterol/dipalmitoylphosphatidylcholine mixtures: 2H nuclear magnetic resonance and differential scanning calorimetry.
Margus R. Vist,James H. Davis +1 more
TL;DR: Deuterium nuclear magnetic resonance spectroscopy and differential scanning calorimetry are used to map the phase boundaries of mixtures of cholesterol and chain-perdeuteriated 1,2-dipalmitoyl-sn-glycero-3-phosphocholine at concentrations from 0 to 25 mol % cholesterol.