Differences in antigen presentation to MHC class I-and class II-restricted influenza virus-specific cytolytic T lymphocyte clones.
Lynda A. Morrison,Aron E. Lukacher,Vivian L. Braciale,David P. Fan,David P. Fan,T J Braciale +5 more
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Treatment of the target cells with the lysosomotropic agent chloroquine abolished recognition of infected target cells by class II-restricted CTL without diminishing class I-restricted recognition ofinfected target cells, suggesting that important differences may exist in requirements for antigen presentation between H-2K/D and H- 2I region- restricted CTL.Abstract:
We have examined requirements for antigen presentation to a panel of MHC class I-and class II-restricted, influenza virus-specific CTL clones by controlling the form of virus presented on the target cell surface. Both H-2K/D- and I region-restricted CTL recognize target cells exposed to infectious virus, but only the I region-restricted clones efficiently lysed histocompatible target cells pulsed with inactivated virus preparations. The isolated influenza hemagglutinin (HA) polypeptide also could sensitize target cells for recognition by class II-restricted, HA-specific CTL, but not by class I-restricted, HA-specific CTL. Inhibition of nascent viral protein synthesis abrogated the ability of target cells to present viral antigen relevant for class I-restricted CTL recognition. Significantly, presentation for class II-restricted recognition was unaffected in target cells exposed to preparations of either inactivated or infectious virus. This differential sensitivity suggested that these H-2I region-restricted CTL recognized viral polypeptides derived from the exogenously introduced virions, rather than viral polypeptides newly synthesized in the infected cell. In support of this contention, treatment of the target cells with the lysosomotropic agent chloroquine abolished recognition of infected target cells by class II-restricted CTL without diminishing class I-restricted recognition of infected target cells. Furthermore, when the influenza HA gene was introduced into target cells without exogenous HA polypeptide, the target cells that expressed the newly synthesized protein product of the HA gene were recognized only by H-2K/D-restricted CTL. These observations suggest that important differences may exist in requirements for antigen presentation between H-2K/D and H-2I region-restricted CTL. These differences may reflect the nature of the antigenic epitopes recognized by these two CTL subsets.read more
Citations
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MHC-dependent antigen processing and peptide presentation: Providing ligands for T lymphocyte activation
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Altered immunoglobulin expression and functional silencing of self-reactive B lymphocytes in transgenic mice
Christopher C. Goodnow,Jeffrey Crosbie,Stephen Adelstein,Thomas B. Lavoie,Sandra J. Smith-Gill,Robert Brink,Helen Pritchard-Briscoe,John S. Wotherspoon,Robert H Loblay,Kathy Raphael,Ronald J. Trent,Antony Basten +11 more
TL;DR: Findings indicate that self tolerance may result from mechanisms other than clonal deletion, and are consistent with the hypothesis that IgD may have a unique role in B-cell tolerance.
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Characterization of peptides bound to the class I MHC molecule HLA-A2.1 by mass spectrometry
Donald F. Hunt,Robert A. Henderson,Jeffrey Shabanowitz,Kazuyasu Sakaguchi,Hanspeter Michel,Noelle Sevilir,Andrea L. Cox,Ettore Appella,Victor H. Engelhard +8 more
TL;DR: Microcapillary high-performance liquid chromatography-electrospray ionization-tandem mass spectrometry was used to fractionate and sequence subpicomolar amounts of peptides isolated from the MHC molecule HLA-A2.1.
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Role of bone marrow-derived cells in presenting MHC class I-restricted tumor antigens
Alex Yee-Chen Huang,Paul Golumbek,Mojgan Ahmadzadeh,Elizabeth M. Jaffee,Drew M. Pardoll,Hyam I. Levitsky +5 more
TL;DR: MHC class I-restricted antigens are efficiently transferred in vivo to bone marrow-derived antigen-presenting cells, which suggests that human leukocyte antigen matching may be less critical in the application of tumor vaccines than previously thought.
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Introduction of soluble protein into the class I pathway of antigen processing and presentation
TL;DR: C57BL/6 mice immunized against a syngeneic tumor cell transfected with chicken ovalbumin cDNA gave rise to H-2Kb-restricted CTL specific for the OVA258-276 peptide, which was able to target H- 2b cells for lysis by the CTL in a 3 hr assay.
References
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