Journal ArticleDOI
DNA sequence organization of the β-globin complex in the BALB/c mouse
Carolyn L. Jahn,Clyde A. Hutchison,Sandra J. Phillips,Steven Weaver,Nancy L. Haigwood,Charles F. Voliva,Marshall H. Edgell +6 more
TLDR
Clones containing five non-adult β-globin genes were isolated from a library of BALB/c DNA and formed a contiguous block of 32 kb of the mouse genome, establishing the physical linkage of these non- adult genes to the two adult β- globin genes as well as of the twoadult genes to each other.About:
This article is published in Cell.The article was published on 1980-08-01. It has received 141 citations till now. The article focuses on the topics: Gene & Restriction map.read more
Citations
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Journal ArticleDOI
The structure and evolution of the human β-globin gene family
Argiris Efstratiadis,James W. Posakony,Tom Maniatis,Richard M. Lawn,Catherine O'Connell,Richard A. Spritz,Jon K. deRiel,Bernard G. Forget,Sherman M. Weissman,Jerry L. Slightom,Ann E. Blechl,Oliver Smithies,Francisco E. Baralle,Carol C. Shoulders,Nick J. Proudfoot +14 more
TL;DR: A model for the involvement of short direct repeat sequences in the generation of deletions in the noncoding and coding regions of B-like globin genes during evolution is described.
The Structure and Evolution of the Human /?-Globin Gene Family
Argiris Efstratiadis,James W. Posakony,Tom Maniatis,Richard M. Lawn,Richard A. Spritz,Jon K. deRiel,Forget Bg,Sherman M. Weissman,Jerry L. Slightom,Ann E. Blechl,Oliver Smithies,F.E. Baralle,Carol C. Shoulders,Nicholas J. ProudfootQ +13 more
TL;DR: Efstratiadis et al. as mentioned in this paper presented the results of a detailed comparison of the primary structure of human p-like globin genes and their flanking sequences.
Book ChapterDOI
Highly repeated sequences in mammalian genomes.
TL;DR: This chapter discusses the structure and organization of mammalian, highly repeated sequences at the molecular level with a description of tandemly repeated sequences, that are, satellites, and the segments that are interspersed among other genomic DNA sequences.
Journal ArticleDOI
Evidence for involvement of activin A and bone morphogenetic protein 4 in mammalian mesoderm and hematopoietic development.
B M Johansson,M V Wiles +1 more
TL;DR: It is shown that mouse embryonic stem cells, which resemble primitive ectoderm, can differentiate to mesoderm in vitro in a chemically defined medium (CDM) in the absence of fetal bovine serum, and this differentiation is responsive to TGF-beta family members in a concentration-dependent manner.
Journal ArticleDOI
Patterns of Nucleotide Substitution in Pseudogenes and Functional Genes
TL;DR: The pattern obtained suggests that transition mutations occur somewhat more frequently than transversion mutations and that mutations result more often in A or T than in G or C.
References
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Journal ArticleDOI
DNA sequencing with chain-terminating inhibitors
TL;DR: A new method for determining nucleotide sequences in DNA is described, which makes use of the 2',3'-dideoxy and arabinon nucleoside analogues of the normal deoxynucleoside triphosphates, which act as specific chain-terminating inhibitors of DNA polymerase.
Journal ArticleDOI
Detection of specific sequences among DNA fragments separated by gel electrophoresis.
TL;DR: This paper describes a method of transferring fragments of DNA from agarose gels to cellulose nitrate filters that can be hybridized to radioactive RNA and hybrids detected by radioautography or fluorography.
Book ChapterDOI
Sequencing end-labeled DNA with base-specific chemical cleavages.
Allan M. Maxam,Walter Gilbert +1 more
TL;DR: The chapter presents techniques for producing discrete DNA fragments, end-labeling DNA, segregating end- labeled fragments, extracting DNA from gels, and the protocols for partially cleaving it at specific bases using the chemical reactions.
Journal ArticleDOI
Screening lambdagt recombinant clones by hybridization to single plaques in situ
WD Benton,Ronald W. Davis +1 more
TL;DR: A rapid, direct method for screening single plaques of Agt recombinant phage is described, which allows at least 10(6) clones to be screened per day and simplifies physical containment of recombinants.