Effect of tea catechins on cellular lipid peroxidation and cytotoxicity in HepG2 cells.
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TLDR
EGCG and EGC protected the depletion of α-tocopherol in the cells, and the glutathione content was enhanced by all four catechins.Abstract:
Tea catechins inhibited TBARS accumulation in HepG2 cells, the order of effectiveness being (−)-epigallocatechin gallate (EGCG)>(−)-epigallocatechin (EGC)≥(−)-epicatechin gallate (ECG)>(−)-epicatechin (EC). EGCG and EGC protected the depletion of α-tocopherol in the cells, and the glutathione content was enhanced by all four catechins. Moreover, all four catechins suppressed the formation of glutathione disulfide and the activation of glutathione peroxidase induced by tert-butylated hydroperoxide.read more
Citations
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Green tea catechin, epigallocatechin-3-gallate (EGCG): mechanisms, perspectives and clinical applications.
TL;DR: Green tea catechin has great potential in cancer prevention because of its safety, low cost and bioavailability, and its mechanism of action at numerous points regulating cancer cell growth, survival, angiogenesis and metastasis.
Journal ArticleDOI
Oxidative stress and dietary phytochemicals: Role in cancer chemoprevention and treatment
Shireen Chikara,Lokesh Dalasanur Nagaprashantha,Jyotsana Singhal,David Horne,Sanjay Awasthi,Sharad S. Singhal +5 more
TL;DR: Multiple studies investigating the role of dietary phytochemicals such as, curcumin, epigallocatechin gallate, resveratrol, phenethyl isothiocyanate, sulforaphane, hesperidin, quercetin and 2'-hydroxyflavanone in regulating oxidative stress and associated signaling pathways in the context of cancer chemoprevention and treatment are reviewed.
Journal ArticleDOI
Use of a human-derived liver cell line for the detection of cytoprotective, antigenotoxic and cogenotoxic agents.
TL;DR: HepG2 cells are of great relevance to detect cytotoxic and genotoxic substances and by extension cytoprotective, antigenotective and cogenotoxic agents.
Journal ArticleDOI
Antioxidant mechanism of tea polyphenols and its impact on health benefits.
TL;DR: The application of tea polyphenols in human and animal health is examined, providing a scientific basis for this application in addition to proposing future directions for the development of this resource.
Journal ArticleDOI
Quercetin protects human hepatoma HepG2 against oxidative stress induced by tert-butyl hydroperoxide
TL;DR: The results of the biomarkers analyzed clearly show that treatment of HepG2 cells in culture with the natural dietary antioxidant quercetin strongly protects the cells against an oxidative insult.
References
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Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction
TL;DR: Using this method, the liped peroxide level in the liver of rats suffering from carbon tetrachloride intoxication was investigated and was in good agreement with previously reported data obtained by measuring diene content.
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Structure-antioxidant activity relationships of flavonoids and phenolic acids
TL;DR: The factors underlying the influence of the different classes of polyphenols in enhancing their resistance to oxidation are discussed and support the contention that the partition coefficients of the flavonoids as well as their rates of reaction with the relevant radicals define the antioxidant activities in the lipophilic phase.
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Dietary antioxidant flavonoids and risk of coronary heart disease: the Zutphen Elderly Study.
TL;DR: Flavonoids in regularly consumed foods may reduce the risk of death from coronary heart disease in elderly men and showed an inverse relation with incidence of myocardial infarction.
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Polyphenolic Flavanols as Scavengers of Aqueous Phase Radicals and as Chain-Breaking Antioxidants
N. Salah,Nicholas J. Miller,George Paganga,Lilian B. M. Tijburg,G.P. Bolwell,Catherine Rice-Evans +5 more
TL;DR: Against propagating lipid peroxyl radical species, epicatechin and catechin are as effective as ECG and EGCG, the least efficacious being EGC and GA.
Journal ArticleDOI
Microtiter plate assay for the measurement of glutathione and glutathione disulfide in large numbers of biological samples.
TL;DR: By combining the least complicated and expedient methods of sample handling with the sensitivity and specificity of the GSH assay by enzymatic recycling and the small volumes and software capabilities of microtiter plate technology, this work devised a rapid, sensitive, and easy assay for GSH and GSSG in biological samples.
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