Journal ArticleDOI
Effects of calmodulin on the (Ca2+ + Mg2+)ATPase partially purified from erythrocyte membranes.
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TLDR
A comparison of two types of ghosts showed that isotonic ghosts possess an intact calmodulin-(Ca 2+ + Mg 2+ )ATPase complex, and that theCalmodulin remained with the ATPase during solubilization and reconstitution.About:
This article is published in Archives of Biochemistry and Biophysics.The article was published on 1979-11-01. It has received 51 citations till now. The article focuses on the topics: Calmodulin & ATPase complex.read more
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Journal ArticleDOI
Purified (Ca2+-Mg2+)-ATPase of the erythrocyte membrane. Reconstitution and effect of calmodulin and phospholipids.
TL;DR: The purified and the reconstituted forms of the enzyme displayed the properties expected of the intact Ca2+ pump; they had an appropriate (Ca2+-Mg2+)-ATPase activity which displayed a relatively low affinity for Ca2+.
Journal ArticleDOI
Acidic phospholipids, unsaturated fatty acids, and limited proteolysis mimic the effect of calmodulin on the purified erythrocyte Ca2+ - ATPase.
TL;DR: The isolated enzyme, after reconstitution in phosphatidylcholine liposomes in the absence of calmodulin, can be activated by limited proteolysis and the trypsinized enzyme has the same high Vmax and high affinity for Ca2+ of the enzyme in the presence of Calmodulin.
Journal ArticleDOI
Interaction of drugs with calmodulin. Biochemical, pharmacological and clinical implications.
TL;DR: The fundamental role calmodulin plays in biology suggests that this calcium binding protein may provide a new site for the pharmacological manipulation of biological activity, and the inhibitors described hardly scratch the surface of this fertile area of research.
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Journal ArticleDOI
The preparation and chemical characteristics of hemoglobin-free ghosts of human erythrocytes
TL;DR: The effects of the ionic strength and pH of the hemolyzing solution on the hemoglobin content of human erythrocyte ghosts were studied in phosphate buffers and suggest an electrophysical interaction of hemoglobin with membrane constituents.
Journal ArticleDOI
Assay of proteins in the presence of interfering materials.
TL;DR: The Lowry protein assay is a sensitive but highly nonspecific procedure that has been modified so that protein can be assayed in the presence of interfering chemicals.
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Cyclic 3',5'-nucleotide phosphodiesterase. Demonstration of an activator.
W Y Cheung,W Y Cheung +1 more
TL;DR: In this paper, a mixture of a purified and a crude cyclic 3′,5′-nucleotide phosphodiesterase was shown to have higher activity than the summed activities of the individual preparations.