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Effects of ozone, chlorine dioxide, chlorine, and monochloramine on Cryptosporidium parvum oocyst viability.

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TLDR
The data indicate that C. parvum oocysts are 30 times moreresistant to ozone and 14 times more resistant to chlorine dioxide than Giardia cysts exposed to these disinfectants under the same conditions.
Abstract
Purified Cryptosporidium parvum oocysts were exposed to ozone, chlorine dioxide, chlorine, and monochloramine. Excystation and mouse infectivity were comparatively evaluated to assess oocyst viability. Ozone and chlorine dioxide more effectively inactivated oocysts than chlorine and monochloramine did. Greater than 90% inactivation as measured by infectivity was achieved by treating oocysts with 1 ppm of ozone (1 mg/liter) for 5 min. Exposure to 1.3 ppm of chlorine dioxide yielded 90% inactivation after 1 h, while 80 ppm of chlorine and 80 ppm of monochloramine required approximately 90 min for 90% inactivation. The data indicate that C. parvum oocysts are 30 times more resistant to ozone and 14 times more resistant to chlorine dioxide than Giardia cysts exposed to these disinfectants under the same conditions. With the possible exception of ozone, the use of disinfectants alone should not be expected to inactivate C. parvum oocysts in drinking water.

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Citations
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In Pediatric Gastroenteritis: the Presence of Helicobacter Pylori Prevalence of Entamoeba Histolytica, Giardia Intestinalis And Cryptosporidium Spp

TL;DR: E. histolytica, G. intestinalis and Cryptosporidium spp were identified as important pathogens commonly seen in Erzurum and adequate hygienic measures should be taken to reduce the frequency of enteric pathogens.
Journal ArticleDOI

Water‐borne cryptosporidiosis: a recurring problem

TL;DR: It is stated that the presence of Cryptosporidium oocysts in potable water supplies continues to be a high profile problem in the UK and the USA, causing concern among public health officials and those responsible for water treatment.
References
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Book

Standard methods for the examination of water and wastewater

TL;DR: The most widely read reference in the water industry, Water Industry Reference as discussed by the authors, is a comprehensive reference tool for water analysis methods that covers all aspects of USEPA-approved water analysis.
Journal ArticleDOI

Determination of ozone in water by the indigo method

TL;DR: In this article, the decolorization of indigo trisulfonate (600 nm, pH below 4) was used to determine the concentration of aqueous ozone in the range 0.005 −30 mg 1−1.
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Acute enterocolitis in a human being infected with the protozoan Cryptosporidium.

TL;DR: It is suggested, on the basis of the severity of the clinical symptoms, and on the pathological changes in the rectum, that the organism in this case is likely to have been the cause of the enterocolitis and thus to have was a pathogen rather than a commensal.
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Isolation of Cryptosporidium oocysts and sporozoites using discontinuous sucrose and isopycnic Percoll gradients.

TL;DR: Techniques for the large-scale isolation of Cryptosporidium oocysts and sporozoites, obtained from the feces of experimentally infected Holstein calves, were developed employing discontinuous sucrose gradients and isopycnic Percoll gradients.
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Large Community Outbreak of Cryptosporidiosis Due to Contamination of a Filtered Public Water Supply

TL;DR: Current standards for the treatment of public water supplies may not prevent the contamination of drinking water by Cryptosporidium, with consequent outbreaks of cryptosporidiosis, it is concluded.
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