Journal ArticleDOI
Efficient 1,3-propanediol Production by Fed-Batch Culture of Klebsiella Pneumoniae : The Role of pH Fluctuation
TLDR
A self-protection mechanism in K. pneumoniae cells switch the metabolic pathways responding to environmental pH changes, which was proposed and fulfilled under the pH-stat fed-batch strategy under the periodical low or high pH stress.Abstract:
The fermentative production of 1,3-propanediol (1,3-PD) by Klebsiella pneumoniae under different fed-batch strategies was investigated. pH-stat fed-batch strategies proved to be not effective for economical 1,3-PD production for the existence of relatively high concentration of byproducts and residual glycerol at the end of the fermentation. However, in the pH-stat fed-batch strategy, an important phenomenon was observed that the yields of two main byproducts, 2,3-butanediol and lactic acid, were closely related to pH value. The dominant byproduct was 2,3-butanediol at a pH value of 5.0 to 6.5 but changed to be lactic acid at a pH value of 7.1 to 8.0. Based on the analysis of the phenomenon, a self-protection mechanism in K. pneumoniae, namely that the growing K. pneumoniae cells switch the metabolic pathways responding to environmental pH changes, was proposed. Thus a kind of feeding strategy was further applied during which the pH value was fluctuated between 6.3 and 7.3 periodically by feeding glycerol-ammonia mixture and sulphuric acid to make the metabolic pathways of 2,3-butanediol and lactic acid sub-active under the periodical low or high pH stress. At last, efficient 1,3-PD production was fulfilled under this fed-batch strategy, and the best results were achieved leading to 70 g/l 1,3-PD with a yield of 0.70 mol/mol glycerol and productivity of 0.97 g/l/h, while the two main byproducts and residual glycerol were under low concentrations.read more
Citations
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Journal ArticleDOI
Microbial 2,3-butanediol production: A state-of-the-art review
TL;DR: Various strategies for efficient and economical microbial 2,3-butanediol production, including strain improvement, substrate alternation, and process development, are reviewed and compared with regard to their pros and cons.
Journal ArticleDOI
Microbial production of diols as platform chemicals: recent progresses.
An-Ping Zeng,Wael Sabra +1 more
TL;DR: The recent development in the microbial production of these diols is reviewed, especially regarding the engineering of production strains and optimization of the fermentation processes.
Journal ArticleDOI
Advances in biotechnological production of 1,3-propanediol
TL;DR: This review presents the “state of the art” in the biotechnological production technologies of 1,3-PD particularly with respect to bioprocess engineering methods and highlights the significance of mathematical model-based approach for designing various bioreactor operating strategies to facilitate the improvement in 1, 3-PD production.
Journal ArticleDOI
Enhanced 1,3-propanediol production by a newly isolated Citrobacter freundii strain cultivated on biodiesel-derived waste glycerol through sterile and non-sterile bioprocesses.
TL;DR: The production of 1,3-propanediol (PD) by a newly isolated Citrobacter freundii strain [FMCC-B 294 (VK-19) was investigated and the capability of the strain to tolerate elevated amounts of substrate and produce quantities of PD in such high substrate concentrations was revealed.
Journal ArticleDOI
Enhanced production of 2,3-butanediol from glycerol by forced pH fluctuations
Kaloyan Petrov,Penka Petrova +1 more
TL;DR: The optimal conditions for maximal glycerol consumption were defined, and 70 g/l 2,3-BD were produced, which is the highest amount obtained from Glycerol as a sole carbon source until now, by the development of a new method of “forced pH fluctuations”.
References
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Journal ArticleDOI
The path forward for biofuels and biomaterials
Arthur J. Ragauskas,Charlotte K. Williams,Brian H. Davison,George J. P. Britovsek,John Cairney,Charles A. Eckert,William J. Frederick,Jason P. Hallett,David J. Leak,Charles L. Liotta,Jonathan R. Mielenz,Richard J. Murphy,Richard H. Templer,Timothy J. Tschaplinski +13 more
TL;DR: The integration of agroenergy crops and biorefinery manufacturing technologies offers the potential for the development of sustainable biopower and biomaterials that will lead to a new manufacturing paradigm.
Journal ArticleDOI
Microbial production of 1,3-propanediol.
TL;DR: Great efforts are now being made to combine the pathway from glucose to glycerol successfully with the bacterial route from Glycerol to 1,3-PD, which may become the first bulk chemical produced by a genetically engineered microorganism.
Journal ArticleDOI
Glycerol production by microbial fermentation: A review
TL;DR: Improvements have been made in the glycerol production using osmotolerant yeasts on a commercial scale in China in recent years and a carrier distillation technique with a Glycerol distillation efficiency greater than 90% has been developed.
Journal ArticleDOI
Biotechnological valorisation of raw glycerol discharged after bio-diesel (fatty acid methyl esters) manufacturing process: Production of 1,3-propanediol, citric acid and single cell oil
Seraphim Papanikolaou,Seraphim Papanikolaou,Stylianos Fakas,Stylianos Fakas,Michel Fick,Isabelle Chevalot,Maria Galiotou-Panayotou,Michael Komaitis,Ivan Marc,George Aggelis +9 more
TL;DR: Fatty acid analysis of total cellular lipids showed that glycerol concentration increase in the growth medium somehow increased the cellular unsaturated fatty acids content of lipids.
Book ChapterDOI
Bulk chemicals from biotechnology: the case of 1,3-propanediol production and the new trends.
An-Ping Zeng,Hanno Biebl +1 more
TL;DR: The microbial production of 1,3-propanediol as briefly reviewed in this article and compared with the competing chemical processes demonstrates the promise and constraints of bioprocesses for bulk chemicals.
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