Journal ArticleDOI
Enhanced Chemotherapy Efficacy by Sequential Delivery of siRNA and Anticancer Drugs Using PEI‐Grafted Graphene Oxide
TLDR
It is demonstrated that the PEI-GO is an excellent nanocarrier for effective delivery of siRNA and chemical drugs, and that sequential delivery of the siRNAs and the anticancer drug doxorubicin by PEi-GO into cancer cells exhibits a synergistic effect, which leads to a signifi cantly enhanced chemotherapy effi cacy.Abstract:
PEI GO The RNA interference (RNAi) technique, an effective method to inhibit protein expression by targeted cleavage of messenger RNA (mRNA), has made substantial progress since the fi rst demonstration of gene knockdown in mammalian cells. [ 1 ] Short interfering RNA (siRNA) induces specifi c silencing of targeted protein, thus offering signifi cant potential in overcoming multiple drug resistance (MDR) of cancer cells. [ 2 ] For example, Bcl-2 protein, one of the main antiapoptotic defense proteins, is closely related to the MDR of cancer cells. [ 3 ] Knockdown of the Bcl-2 protein expression level in cancer cells by Bcl-2-targeted siRNA would effectively overcome the MDR of cancer cells and sensitize cancer cells to anticancer drugs. [ 3 d, 4 ] Herein, we report sequential delivery of Bcl-2-targeted siRNA and the anticancer drug doxorubicin (DOX) using polyethylenimine (PEI)-functionalized graphene oxide (PEI-GO). We demonstrate that the PEI-GO is an excellent nanocarrier for effective delivery of siRNA and chemical drugs, and that sequential delivery of the siRNA and DOX by PEI-GO into cancer cells exhibits a synergistic effect, which leads to a signifi cantly enhanced chemotherapy effi cacy. To the best of our knowledge, this is the fi rst report on applications of GO-based nanovectors for delivery of siRNA, and sequential delivery of siRNA and anticancer drugs into cancer cells. Graphene, a newly discovered 2D nanomaterial, has been studied extensively due to its fundamental importance and potential applications, [ 5 ] while exploration of its biomedical applications has just started. [ 6 ] Noncovalent adsorption through π – π stacking, electrostatic, and other molecular interactions has proven to be effective for immobilizing chemical drugs, single-stranded DNA, and RNA onto GO sheets. [ 6 a–e]read more
Citations
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Journal ArticleDOI
Functional Nanomaterials for Phototherapies of Cancer
Journal ArticleDOI
Nano-graphene in biomedicine: theranostic applications
TL;DR: Graphene-based photothermal therapy has been realized, achieving excellent anti-tumor therapeutic efficacy in animal experiments and future prospects and challenges of using graphene-based materials for theranostic applications are discussed.
Journal ArticleDOI
Biomedical Applications of Graphene and Graphene Oxide
TL;DR: Recent efforts to apply graphene and graphene oxides (GO) to biomedical research and a few different approaches to prepare graphene materials designed for biomedical applications are reviewed.
Journal ArticleDOI
Graphene-based nanomaterials for drug delivery and tissue engineering.
TL;DR: This article presents a comprehensive review of various types and properties of graphene family nanomaterials and highlights how these properties are being exploited for drug delivery and tissue engineering applications.
Journal ArticleDOI
Graphene and graphene oxide as new nanocarriers for drug delivery applications.
TL;DR: An overview of recent advances in graphene-based nanocarriers, their biocompatibility and toxicity, followed by a summary of the most appealing examples demonstrated for the delivery of anti-cancer drugs and genes are presented.
References
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Sasha Stankovich,Dmitriy A. Dikin,Geoffrey Dommett,K. Kohlhaas,Eric Zimney,Eric A. Stach,Richard D. Piner,SonBinh T. Nguyen,Rodney S. Ruoff +8 more
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TL;DR: 21-nucleotide siRNA duplexes provide a new tool for studying gene function in mammalian cells and may eventually be used as gene-specific therapeutics.
Journal ArticleDOI
A versatile vector for gene and oligonucleotide transfer into cells in culture and in vivo: polyethylenimine
Otmane Boussif,Frank Lezoualc'h,Maria Antonietta Zanta,Mojgan Mergny,Daniel Scherman,Barbara A. Demeneix,Jean-Paul Behr +6 more
TL;DR: Together, these properties make PEI a promising vector for gene therapy and an outstanding core for the design of more sophisticated devices because its efficiency relies on extensive lysosome buffering that protects DNA from nuclease degradation, and consequent lysOSomal swelling and rupture that provide an escape mechanism for the PEI/DNA particles.
Journal ArticleDOI
Graphene: The New Two-Dimensional Nanomaterial
TL;DR: The status of graphene research is presented, which includes aspects related to synthesis, characterization, structure, and properties.