Journal ArticleDOI
Enzymic determination of uracil nucleotides in tissues.
TLDR
An enzymic assay for the sequential determination of UDP-glucose, UDP-galactose, UTP, UDP, and 5′-UMP in tissues is described, which uses commercially available enzymes and is highly reproducible, with standard deviations of less than 2%.About:
This article is published in Analytical Biochemistry.The article was published on 1970-11-01. It has received 123 citations till now. The article focuses on the topics: Uracil nucleotide & Uracil.read more
Citations
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Journal ArticleDOI
Effect of infection with enveloped viruses on nucleotide metabolism.
TL;DR: In Semliki Forest virus-infected BHK cells the stimulation of the uptake of uridine is not only caused by changes of the membrane function but also by the release of a negative feedback regulation controlled by the UTP pool.
Journal ArticleDOI
A method for measuring free-nucleotide pool sizes and incorporation of labeled precursors: its application in studying myocardial pyrimidine nucleotides.
TL;DR: A procedure for a rapid and accurate determination of nucleotide pool sizes in heart muscle is described, which permits reproducible measurements of the pools of pyrimidine nucleotides which are particularly small in cardiac tissue.
Journal Article
Plasma Membrane Changes of Liver and Morris Hepatoma Induced by Retinol in Rats
Reinhard Büchsel,Werner Reutter +1 more
TL;DR: It is indicated that the increased hepatic secretory activity after high doses of retinol leads to rapid turnover of incompletely fucosylated glycoproteins of the plasma membrane, which is related to malignant transformation and not to increased growth.
Book ChapterDOI
Über die primäre und sekundäre biochemische Antwort der Leber nach Gabe von D-Galaktosamin beim Zustandekommen der Galaktosaminhepatitis
TL;DR: In this article, the Galaktosamin-Gabe wird innerhalb von ≤ 6 Std in Ratten ein Hepatitis erzeugt.
Journal ArticleDOI
Modulation of rat tissue galactose-1-phosphate uridyltransferase by uridine and uridine triphosphate
Shirley Rogers,Stanton Segal +1 more
TL;DR: Uridine and UTP at low concentrations were found to have an additive effect on rat hepatic transferase activity, which is especially significant in that uridine administration is known to increase hepatic UTP concentration.
References
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Journal ArticleDOI
Eine einfache Technik der extrem schnellen Abkühlung größerer Gewebestücke
TL;DR: In this article, a pair of tongs are used to compress large tissue pieces and organs of animals to a thin layer and can thus be frozen in a fraction of a second.
Journal ArticleDOI
Experimental hepatitis induced by d-galactosamine
TL;DR: Repeated intraperitoneal injections of d-galactosamine produce within 24–48 hours in rat livers alterations that closely resemble human viral hepatitis, indicating a high specificity of d -galactoamine.
Journal ArticleDOI
The enzymatic transformation of uridine diphosphate glucose into a galactose derivative
TL;DR: It is concluded that the compound found with treatment of UDPG with an enzyme of S. fragilis is uridine diphosphate galactose, and the bearing of this finding on the mechanism of action of UDPG is discussed.
Journal ArticleDOI
Isolation of the coenzyme of the galactose phosphate-glucose phosphate transformation.
TL;DR: In the initial studies (5) it was found that a thermostable factor was required in the over-all Reactions II and III, which led eventually to the discovery and isolation of glucose diphosphate, which acts as a coenzyme in Reaction III.
Related Papers (5)
The Trapping of Uridine Phosphates by d-Galactosamine, d-Glucosamine, and 2-Deoxy-d-galactose
Selective Uridine Triphosphate Deficiency Induced by d-Galactosamine in Liver and Reversed by Pyrimidine Nucleotide Precursors EFFECT ON RIBONUCLEIC ACID SYNTHESIS
Studies on the Mechanism of Galactosamine Hepatitis: Accumulation of Galactosamine‐1‐Phosphate and its Inhibition of UDP‐Glucose Pyrophosphorylase
D. Keppler,K. Decker +1 more