Journal ArticleDOI
Enzymic determination of uracil nucleotides in tissues.
TLDR
An enzymic assay for the sequential determination of UDP-glucose, UDP-galactose, UTP, UDP, and 5′-UMP in tissues is described, which uses commercially available enzymes and is highly reproducible, with standard deviations of less than 2%.About:
This article is published in Analytical Biochemistry.The article was published on 1970-11-01. It has received 123 citations till now. The article focuses on the topics: Uracil nucleotide & Uracil.read more
Citations
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Journal ArticleDOI
Comparaison des teneurs en saccharose et en nucléotides riches en énergie des graines en germination d'une betterave sucrière et d'une betterave fourragère
TL;DR: Comparison of a sugar beet with a forage beet variety shows that, during germination, the seedlings have the same nucleoside triphosphate content, but those of sugar beet contain more sucrose and less UDPG than those of forage Beet.
Journal ArticleDOI
Increased uracil nucleotide metabolism during the induction of cardiac hypertrophy by β-stimulation in rats@@@Gesteigerter Uracil-Nucleotid-Metabolismus während der Erzeugung einer Herzhypertrophie durch β-Rezeptoren-Stimulierung bei der Ratte
Journal ArticleDOI
Adaptation of rats to a diet high in galactose
A. Cryer,W. Hartley +1 more
TL;DR: Rats fed galactose as 70 per cent of their diet first lost weight but afterwards grew at one-sixth of the normal rate, and urine production was nine to sixteen times higher than in the controls.
Journal ArticleDOI
Inhibition of tyrosine aminotransferase induction by UTP deficiency and its reversal by 5-fluorouridine in cultured hepatoma cells.
TL;DR: Hepatoma tissue culture cells, grown in the presence of D-galactosamine and 6-azauridine, demonstrate a strong reduction of the intracellular UTP pool that can be replenished by formation of UTP from uridine and FUTP from 5-fluorouridine within 2 h.
Book ChapterDOI
[31] Luminometric determination of flavin adenine dinucleotide
Karl Decker,Ari Hinkkanen +1 more
TL;DR: This chapter highlights luminometric determination of flavin adenine dinucleotide (FAD), the predominant biologically active form of riboflavin, which is an ubiquitous cofactor of redox enzymes such as dehydrogenases, transhydrogenase, reductases, oxidases, and oxygenases.
References
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Journal ArticleDOI
Eine einfache Technik der extrem schnellen Abkühlung größerer Gewebestücke
TL;DR: In this article, a pair of tongs are used to compress large tissue pieces and organs of animals to a thin layer and can thus be frozen in a fraction of a second.
Journal ArticleDOI
Experimental hepatitis induced by d-galactosamine
TL;DR: Repeated intraperitoneal injections of d-galactosamine produce within 24–48 hours in rat livers alterations that closely resemble human viral hepatitis, indicating a high specificity of d -galactoamine.
Journal ArticleDOI
The enzymatic transformation of uridine diphosphate glucose into a galactose derivative
TL;DR: It is concluded that the compound found with treatment of UDPG with an enzyme of S. fragilis is uridine diphosphate galactose, and the bearing of this finding on the mechanism of action of UDPG is discussed.
Journal ArticleDOI
Isolation of the coenzyme of the galactose phosphate-glucose phosphate transformation.
TL;DR: In the initial studies (5) it was found that a thermostable factor was required in the over-all Reactions II and III, which led eventually to the discovery and isolation of glucose diphosphate, which acts as a coenzyme in Reaction III.
Related Papers (5)
The Trapping of Uridine Phosphates by d-Galactosamine, d-Glucosamine, and 2-Deoxy-d-galactose
Selective Uridine Triphosphate Deficiency Induced by d-Galactosamine in Liver and Reversed by Pyrimidine Nucleotide Precursors EFFECT ON RIBONUCLEIC ACID SYNTHESIS
Studies on the Mechanism of Galactosamine Hepatitis: Accumulation of Galactosamine‐1‐Phosphate and its Inhibition of UDP‐Glucose Pyrophosphorylase
D. Keppler,K. Decker +1 more