Journal ArticleDOI
Enzymic determination of uracil nucleotides in tissues.
TLDR
An enzymic assay for the sequential determination of UDP-glucose, UDP-galactose, UTP, UDP, and 5′-UMP in tissues is described, which uses commercially available enzymes and is highly reproducible, with standard deviations of less than 2%.About:
This article is published in Analytical Biochemistry.The article was published on 1970-11-01. It has received 123 citations till now. The article focuses on the topics: Uracil nucleotide & Uracil.read more
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Changes in myocardial pyrimidine nucleotide levels following repeated injections of isoproterenol in rats
TL;DR: Changes in the pool sizes of adenine, uracil and cytosine nucleotides were observed during the initial phase of cardiac overload and the significance of an increase in the pyrimidine nucleotide pools in relation to nucleic acid synthesis is discussed.
Journal ArticleDOI
ATP-induced calcium increase as a potential first signal in mechanical tissue trauma. A laser scanning microscopic study on cultured mouse skeletal myocytes.
Uta Kerkweg,Herbert de Groot +1 more
TL;DR: A decisive role of ATP and related nucleotides in the pathogenesis of tissue trauma is suggested because they appear to initiate the signaling mechanism from injured myocytes to the surrounding tissue and potentially to the whole body.
Journal ArticleDOI
Ganglioside biosynthesis in rat liver: alteration of sialyltransferase activities by nucleotides.
TL;DR: CMP-NAcNeu:GM3 ganglioside sialyltransferase (GD3 synthase) was characterized with respect to regulation of activity by nucleotides and compared in this regard with other sIALyltransferases of gangLioside biosynthesis.
Journal ArticleDOI
Increased activities of hepatic orotidine 5′‐phosphate pyrophosphorylase and orotidine 5′‐phosphate decarboxylase induced by orotate
TL;DR: Orotate administration leads to increased activities of the hepatic enzymes catalyzing the conversion of orotate to UMP, which is considered essential for the prevention of galactosamine-induced hepatitis by orotates.
Journal ArticleDOI
Effect of ethanol and fructose on galactose elimination in rat.
TL;DR: The pattern of the metabolites observed in liver tissue is compatible with an inhibitory effect of ethanol on UDP-glucose-4-epimerase, but with the phosphorylation of galactose by galactokinase as the rate-limiting step, inhibited by ethanol.
References
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Journal ArticleDOI
Eine einfache Technik der extrem schnellen Abkühlung größerer Gewebestücke
TL;DR: In this article, a pair of tongs are used to compress large tissue pieces and organs of animals to a thin layer and can thus be frozen in a fraction of a second.
Journal ArticleDOI
Experimental hepatitis induced by d-galactosamine
TL;DR: Repeated intraperitoneal injections of d-galactosamine produce within 24–48 hours in rat livers alterations that closely resemble human viral hepatitis, indicating a high specificity of d -galactoamine.
Journal ArticleDOI
The enzymatic transformation of uridine diphosphate glucose into a galactose derivative
TL;DR: It is concluded that the compound found with treatment of UDPG with an enzyme of S. fragilis is uridine diphosphate galactose, and the bearing of this finding on the mechanism of action of UDPG is discussed.
Journal ArticleDOI
Isolation of the coenzyme of the galactose phosphate-glucose phosphate transformation.
TL;DR: In the initial studies (5) it was found that a thermostable factor was required in the over-all Reactions II and III, which led eventually to the discovery and isolation of glucose diphosphate, which acts as a coenzyme in Reaction III.
Related Papers (5)
The Trapping of Uridine Phosphates by d-Galactosamine, d-Glucosamine, and 2-Deoxy-d-galactose
Selective Uridine Triphosphate Deficiency Induced by d-Galactosamine in Liver and Reversed by Pyrimidine Nucleotide Precursors EFFECT ON RIBONUCLEIC ACID SYNTHESIS
Studies on the Mechanism of Galactosamine Hepatitis: Accumulation of Galactosamine‐1‐Phosphate and its Inhibition of UDP‐Glucose Pyrophosphorylase
D. Keppler,K. Decker +1 more