Evaluation of the loop-mediated isothermal amplification assay for Staphylococcus aureus detection: a systematic review and meta-analysis
TLDR
In this article , a systematic review and meta-analysis were conducted to evaluate the accuracy of the LAMP assay for Staphylococcus aureus detection, and the results showed that both pooled sensitivity and specificity of LAMP were 99% (95% CI 99-100).Abstract:
Staphylococcus aureus can cause many diseases and even death. It's important to detect Staphylococcus aureus rapidly and reliably. The accuracy of a novel test named LAMP in detecting Staphylococcus aureus is unclear. Therefore, a systematic review and meta-analysis were conducted to evaluate the accuracy of the LAMP assay for Staphylococcus aureus detection.Four databases were searched for relevant studies. Meta-DiSc 1.4.0 and Stata 12.0 were used for statistical analysis. At the same time, we used QUADAS-2 to assess the studies we included. Two groups of subgroup analysis were done to differentiate the diagnostic effects of various LAMP tests and in cases of different gold standards.11 studies were identified and 19 2 × 2 contingency tables were extracted in our study. The results showed that both pooled sensitivity and specificity of the LAMP assay were 99% (95% CI 99-100).The LAMP assay demonstrated high sensitivity and specificity in diagnosing Staphylococcus aureus. read more
Citations
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Rapid detection of Staphylococcus aureus using a novel multienzyme isothermal rapid amplification technique
TL;DR: In this study, MIRA assay was developed and validated (specificity; 100%) for the detection of S. aureus with nuc as the target gene, and provides a rapid and easy-to-operate method that can be used for the timely diagnosis and prevention of S-aureus infections.
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Fighting nosocomial antibiotic-resistant infections through rapid and sensitive isothermal amplification-powered point-of-care (POC) diagnostics
M De Felice,Mariarosaria De Falco,Valeria Frisulli,Amina Antonacci,Rachele Isticato,Luca De Stefano,Viviana Scognamiglio +6 more
TL;DR: Point-of-care nucleic acid screening is a crucial clinical practice for addressing nosocomial infections in developed and developing countries, as well as in settings where a centralized laboratory approach encounters limitations as mentioned in this paper .
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TL;DR: A novel method that amplifies DNA with high specificity, efficiency and rapidity under isothermal conditions that employs a DNA polymerase and a set of four specially designed primers that recognize a total of six distinct sequences on the target DNA.
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Staphylococcus aureus and Staphylococcal Food-Borne Disease: An Ongoing Challenge in Public Health
TL;DR: An overview of Staphylococcal food-borne disease, contributing factors, risk that it imposes to the consumers, current research gaps, and preventive measures are provided.
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Loop-Mediated Isothermal Amplification Assay for the Rapid Detection of Staphylococcus aureus
TL;DR: In this paper, a loop-mediated isothermal amplification (LAMP) method targeting the arcC gene of Staphylococcus aureus was developed and evaluated with 119 MRSA strains and 25 non-S.Aureus strains.