Journal ArticleDOI
Evolution of sex determination and the Y chromosome: SRY -related sequences in marsupials
Jamie W. Foster,Francine E. Brennan,Gregory K. Hampikian,Peter N. Goodfellow,Andrew H. Sinclair,Robin Lovell-Badge,Lynne Selwood,Marilyn B. Renfree,Desmond W. Cooper,Jennifer A. Marshall Graves +9 more
TLDR
Comparisons of eutherian and metatherian Y-located SRY sequences suggest rapid evolution of these genes, especially outside the region encoding the DNA-binding HMG box.Abstract:
IN mammals, testis determination is under the control of the testis-determining factor borne by the Y chromosome1,2. SRY, a gene cloned from the sex-determining region of the human Y chromosome, has been equated with the testis-determining factor in man3–5 and mouse6,7. We have used a human SRY probe to identify and clone related genes from the Y chromosome of two marsupial species. Comparisons of eutherian and metatherian Y-located SRY sequences suggest rapid evolution of these genes, especially outside the region encoding the DNA-binding HMG box. The SRY homologues, together with the mouse Ubely homologues8, are the first genes to be identified on the marsupial Y chromosome.read more
Citations
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Journal ArticleDOI
Circular transcripts of the testis-determining gene Sry in adult mouse testis
Blanche Capel,Amanda Swain,Silvia K. Nicolis,Adam Hacker,Michael A. Walter,Peter Koopman,Peter N. Goodfellow,Robin Lovell-Badge +7 more
TL;DR: It is suggested that the circles arise from normal splicing processes as a consequence of the unusual genomic structure surrounding the Sry locus in the mouse.
Journal ArticleDOI
Four Evolutionary Strata on the Human X Chromosome
Bruce T. Lahn,David C. Page +1 more
TL;DR: The ages of individual X-Y gene pairs and the locations of their X members on the X chromosome were found to be highly correlated and age decreased in stepwise fashion from thedistal long arm to the distal short arm in at least four "evolutionary strata".
Journal ArticleDOI
Phylogeny of the SOX family of developmental transcription factors based on sequence and structural indicators.
TL;DR: A robust phylogeny of SOX genes is proposed that reflects their evolutionary history in metazoans and finds support for subdivision of the family into groups A-H, as has been suggested in some previous studies, and for the assignment of two new groups, I and J.
Journal ArticleDOI
A comparison of the properties of Sox-3 with Sry and two related genes, Sox-1 and Sox-2
Jérôme Collignon,Shanthini Sockanathan,Adam Hacker,Michel Cohen-Tannoudji,Dominic Norris,Sohaila Rastan,Milena Stevanovic,Peter N. Goodfellow,Robin Lovell-Badge +8 more
TL;DR: It is demonstrated that Sox-3, as well as Sox-1 and Sox-2, are expressed in the urogenital ridge and that their protein products are able to bind the same DNA sequence motif as Sry in vitro, but with different affinities, suggesting an evolutionary link between the genes.
Journal ArticleDOI
The Promise of Comparative Genomics in Mammals
Stephen J. O'Brien,Marilyn Menotti-Raymond,William J. Murphy,William G. Nash,Johannes Wienberg,Roscoe Stanyon,Neal G. Copeland,Nancy A. Jenkins,James E. Womack,Jennifer A. Marshall Graves +9 more
TL;DR: Comparative genetic assessment expands the utility of dense genetic maps of human, mouse, and rat genomes in gene discovery, in functional genomics, and in tracking the evolutionary forces that sculpted the genome organization of modern mammalian species.
References
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Book
Molecular Cloning: A Laboratory Manual
TL;DR: Molecular Cloning has served as the foundation of technical expertise in labs worldwide for 30 years as mentioned in this paper and has been so popular, or so influential, that no other manual has been more widely used and influential.
Journal ArticleDOI
DNA sequencing with chain-terminating inhibitors
TL;DR: A new method for determining nucleotide sequences in DNA is described, which makes use of the 2',3'-dideoxy and arabinon nucleoside analogues of the normal deoxynucleoside triphosphates, which act as specific chain-terminating inhibitors of DNA polymerase.
Journal ArticleDOI
A gene from the human sex-determining region encodes a protein with homology to a conserved DNA-binding motif
Andrew H. Sinclair,Berta P,Berta P,M. S. Palmer,Hawkins,B. Griffiths,Smith Mj,Foster Jw,Foster Jw,A.-M. Frischauf,Robin Lovell-Badge,Peter N. Goodfellow +11 more
TL;DR: A search of a 35-kilobase region of the human Y chromosome necessary for male sex determination has resulted in the identification of a new gene, termed SRY (for sex-determining region Y) and proposed to be a candidate for the elusive testis-d determining gene, TDF.
Journal ArticleDOI
Male development of chromosomally female mice transgenic for Sry
TL;DR: It is shown that Sry on a 14-kilobase genomic DNA fragment is sufficient to induce testis differentiation and subsequent male development when introduced into chromosomally female mouse embryos.
Journal ArticleDOI
A gene mapping to the sex-determining region of the mouse Y chromosome is a member of a novel family of embryonically expressed genes
John Gubbay,Jérôme Collignon,Peter Koopman,Blanche Capel,Androulla Economou,Andrea Münsterberg,Nigel Vivian,Peter N. Goodfellow,Robin Lovell-Badge +8 more
TL;DR: A gene mapping to the sex-determining region of the mouse Y chromosome is deleted in a line of XY female mice mutant for Tdy, and is expressed at a stage during male gonadal development consistent with its having a role in testis determination.