Expression of M-N#1, a histo-blood group B–like antigen, is strongly up-regulated in nonapoptosing mammary epithelial cells during rat mammary gland involution
TLDR
It is shown that up-regulation of carbohydrate antigens is not a general phenomenon during mammary gland involution, and thus that M-N#1 antigen expression is specifically regulated, and that alpha(1,2)fucosyltransferase A, an enzyme required for M-M-N-1 antigen synthesis, is at least partly responsible for regulated M- N# 1 antigen expression postlactation.Abstract:
Antibodies against the histo-blood group B–like antigen M-N#1 efficiently block the growth in vivo of rat mammary carcinoma cells that bear the antigen (Sleeman et al., 1999, Oncogene 18, 4485–4494). To try to understand the function of the M-N#1 antigen, we investigated when and where the antigen is expressed during the normal function of the rat mammary gland. Expression was virtually only seen during mammary gland involution. Here, strong expression of the antigen was observed in mammary epithelial cells, beginning around 2 days postweaning and lasting throughout the involution process. Dexamethasone treatment of animals postlactation inhibited alveolar collapse and remodeling in the mammary gland but inhibited neither the apoptosis of mammary epithelial cells nor the expression of the M-N#1 antigen. We show that up-regulation of carbohydrate antigens is not a general phenomenon during mammary gland involution, and thus that M-N#1 antigen expression is specifically regulated. Up-regulation of α(1,2)fucosyltransferase A, an enzyme required for M-N#1 antigen synthesis, is at least partly responsible for regulated M-N#1 antigen expression postlactation. Most significantly, we observed that the M-N#1 antigen is virtually exclusively expressed on nonapoptosing epithelial cells in the involuting mammary gland. These data suggest that M-N#1 antigen expression might either provide a survival function and/or be expressed in epithelial cells that are destined to grow and remodel mammary duct structures.read more
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Design of the blood group AB glycotope.
Elena Korchagina,Tatyana V. Pochechueva,Polina Obukhova,Andrey A. Formanovsky,Anne Imberty,Robert Rieben,N. V. Bovin +6 more
TL;DR: A polymeric conjugate is synthesized in such a way that de-N-acetylated A-trisaccharide is attached to a polymer via the nitrogen in position C-2 of the galactosamine residue, in order to test the hypothesis that the supposed AB-epitope should be situated in the part of the molecule that is opposite to the NHAc group of GalNAc residue.
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Galectin-3 is strongly up-regulated in nonapoptosing mammary epithelial cells during rat mammary gland involution.
TL;DR: The data suggest that the up-regulation of galectin-3 in the involuting mammary gland is not only controlled transcriptionally but also regulated posttranscriptionally under the control of systemic glucocorticoid hormones involved in coordinating the involution process.
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Histo-blood group antigen expression and proliferative activity of fibroblasts treated with dental monomers.
Victoria Sarafian,Yordanka Uzunova,Soren Hayrabedyan,P. Ganchevska,M. Filipova,Ivan Filipov,Ludmil Lukanov,Stoyan B Vladimirov +7 more
TL;DR: Novel evidence for altered expression of proliferative antigens and enhanced expression of HBGA B in fibroblasts treated with dental monomers bis-GA and bis-GMA is presented suggesting that these substances affect cell morphology, proliferative activity, and antigenic profile.
References
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Journal Article
Milk accumulation triggers apoptosis of mammary epithelial cells.
TL;DR: These findings suggest a cell death pathway which is independent of the systemic levels of lactogenic hormones but which is triggered by an accumulation of apoptosis-inducing factors in the milk, in the lobulo-alveolar structures or by a physical distortion of secretory epithelial cells generated by the engorgement.
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Apoptosis and Remodeling of Mammary Gland Tissue during Involution Proceeds through p53-independent Pathways'
TL;DR: Examination of the process of involution in the presence and absence of functional p53 in mouse models revealed that involution and remodeling, with the concomitant disappearance of the lobulo-alveolar structures, proceeded normally in the absence offunctional p53.
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Effects of the carbohydrate-binding protein galectin-3 on the invasiveness of human breast carcinoma cells
Nadia Le Marer,R. Colin Hughes +1 more
TL;DR: The results suggest that galectin‐3 could play multiple roles in cell metastasis at an early invasive stage by acting in a paracrine manner to stimulate cell migration through an extracellular matrix, and in later stage cancers in synergy with other mediators of cell‐cell aggregation.
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α1,2Fucosyltransferase increases resistance to apoptosis of rat colon carcinoma cells
Caroline Goupille,Séverine Marionneau,Valérie Bureau,Florence Hallouin,Marc Meichenin,Jézabel Rocher,Jacques Le Pendu +6 more
TL;DR: Results show that increased tumorigenicity mediated by alpha1,2fucosyl-ation is associated to increased resistance to apoptosis and to escape from immune control.