Journal ArticleDOI
Expression of the SV40 promoter in fission yeast: identification and characterization of an AP-1-like factor.
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TLDR
Two AP-1-like binding activities in budding yeast cells are found, one of which appears quite distinct from the binding activity of the product of the budding yeast GCN4 gene, and it is demonstrated that in fission yeast the AP- 1 binding site can act as an upstream activating sequence.About:
This article is published in Cell.The article was published on 1988-05-20. It has received 110 citations till now. The article focuses on the topics: Schizosaccharomyces pombe & Yeast.read more
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Transcriptional regulation in mammalian cells by sequence-specific DNA binding proteins
Pamela J. Mitchell,Robert Tjian +1 more
TL;DR: This review summarizes recent studies that define structural domains for DNA binding and transcriptional activation functions in sequence-specific transcription factors in mammalian DNA binding transcription factors.
Journal ArticleDOI
Foreign gene expression in yeast: a review.
TL;DR: In this article, the authors proposed a method to solve the problem of the "missing link" problem, i.e., "missing links" and "missing connections" problem.
Journal ArticleDOI
The jun proto-oncogene is positively autoregulated by its product, Jun/AP-1
TL;DR: In this paper, the human transcription factor Jun/AP-1 was found to be responsible for increased transcription of different cellular genes in response to tumor promoters, such as TPA, and serum factors.
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Isolation and properties of cDNA clones encoding SRF, a transcription factor that binds to the c-fos serum response element
TL;DR: A cDNA clone of SRF expressed in vitro generates protein that forms complexes indistinguishable from those formed with HeLa cell SRF, as judged by DNA binding specificity and the ability to promote SRE-dependent in vitro transcription.
Journal ArticleDOI
nmt1 of fission yeast. A highly transcribed gene completely repressed by thiamine.
TL;DR: The first fully repressible gene in fission yeast is described and thiamine-mediated transcriptional control has been transferred to the bacterial reporter gene chloramphenicol acetyltransferase.
References
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Book
Molecular Cloning: A Laboratory Manual
TL;DR: Molecular Cloning has served as the foundation of technical expertise in labs worldwide for 30 years as mentioned in this paper and has been so popular, or so influential, that no other manual has been more widely used and influential.
Journal ArticleDOI
Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells.
TL;DR: A series of recombinant genomes which directed expression of the enzyme chloramphenicol acetyltransferase (CAT) in mammalian cells provided a uniquely convenient system for monitoring the expression of foreign DNAs in tissue culture cells.
Journal ArticleDOI
Phorbol ester-inducible genes contain a common cis element recognized by a TPA-modulated trans-acting factor.
Peter Angel,Masayoshi Imagawa,Robert Chiu,Bernd Stein,Richard J. Imbra,Hans J. Rahmsdorf,Carsten Jonat,Peter Herrlich,Michael Karin +8 more
TL;DR: Results strongly suggest that AP-1 is at the receiving end of a complex pathway responsible for transmitting the effects of phorbol ester tumor promoters from the plasma membrane to the transcriptional machinery.
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Purified transcription factor AP-1 interacts with TPA-inducible enhancer elements
TL;DR: It is demonstrated that multiple synthetic copies of the consensus AP-1-binding site can act as TPA-inducible enhancers in various plasmid constructs after transfection into HeLa cells, suggesting that AP- 1 is a transcription factor that functions by interacting with a specific enhancer element, and that its activities may be modulated by treatment of cells with TPA.
Journal ArticleDOI
Regulation of inducible and tissue-specific gene expression
TL;DR: A number of cases of inducible and tissue-specific gene expression involve the activation of preexisting transcription factors, rather than the synthesis of new proteins, which may involve covalent modification of the protein or an allosteric change in its structure.