First molecular identification of the zoonotic parasite Anisakis pegreffii(Nematoda: Anisakidae) in a paraffin-embedded granuloma taken from a case of human intestinal anisakiasis in Italy
Simonetta Mattiucci,Michela Paoletti,Michela Paoletti,Francesco Borrini,Massimo Palumbo,Raffaele Macarone Palmieri,Vincenzo Gomes,Alessandra Casati,Giuseppe Nascetti +8 more
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TLDR
This is the first instance of human intestinal anisakiasis diagnosed using PCR of DNA purified from a fixed eosinophilic granuloma embedded in paraffin, reinforcing the pathological significance of the species A. pegreffii to humans.Abstract:
Anisakiasis is an important fish-borne zoonosis provoked by larval stages of nematodes belonging to the genus Anisakis. The detection and identification of human infections is difficult. This is due to: a) the low specificity of the clinical features and symptomatology related to human infections; b) the paucity of diagnostic features of larvae found in granulomatous lesions characteristic of "invasive anisakiasis"; and c) the lack morphological characters diagnostic at the specific level when larvae of Anisakis are detected. Thus, molecular-based diagnostic approaches are warranted. We have developed a PCR method that amplifies the DNA of Anisakis spp. in fixed paraffin-embedded tissues. This method was applied to a granuloma removed from a human case of intestinal anisakiasis in Italy. Specific primers of the mtDNA cox2 gene were used and sequence analysis was performed according to the procedures already established for species of Anisakis. The sequence obtained (629 bp) was compared with those of the other species of Anisakis which have so far been genetically characterized and with sequences obtained from larval stages of Anisakis collected from the Mediterranean fish Engraulis encrasicolus. This enabled the genetic identification of the larva in the human tissue as A. pegreffii. This is the first instance of human intestinal anisakiasis diagnosed using PCR of DNA purified from a fixed eosinophilic granuloma embedded in paraffin. The case of human anisakiasis presented reinforces the pathological significance of the species A. pegreffii to humans. The molecular/genetic methodological approach based on mtDNA cox2 sequence analysis, described here, can allow easy and rapid identification of Anisakis spp. in formalin-fixed and paraffin embedded tissues removed from cases of either gastric or intestinal human anisakiasis.read more
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Journal ArticleDOI
Anisakiasis and gastroallergic reactions associated with Anisakis pegreffii infection, Italy.
Simonetta Mattiucci,Paolo Fazii,Alba De Rosa,Michela Paoletti,Angelo Salomone Megna,Antonio Glielmo,Maurizio De Angelis,Antonella Costa,Costantino Meucci,Vito Calvaruso,Italo Sorrentini,Giuseppe Palma,Fabrizio Bruschi,Giuseppe Nascetti +13 more
TL;DR: Human cases of gastric anisakiasis caused by the zoonotic parasite Anisakis pegreffii are increasing in Italy.
Journal ArticleDOI
Genetic and morphological approaches distinguish the three sibling species of the Anisakis simplex species complex, with a species designation as Anisakis berlandi n. sp. for A. simplex sp. C (Nematoda: Anisakidae).
Simonetta Mattiucci,Paolo Cipriani,Stephen C. Webb,Michela Paoletti,Federica Marcer,Bruno Bellisario,David I. Gibson,Giuseppe Nascetti +7 more
TL;DR: The morphological and morphometric analyses revealed the presence of morphological features that differed among the 3 biological species, and the tree topologies support the finding that A. simplex (s.s.), A. pegreffii, and A. berlandi n.
Book ChapterDOI
Molecular Epidemiology of Anisakis and Anisakiasis: An Ecological and Evolutionary Road Map.
TL;DR: This review addresses the biodiversity, biology, distribution, ecology, epidemiology, and consumer health significance of the so far known species of Anisakis, both in their natural hosts and in human accidental host populations, worldwide.
Journal ArticleDOI
The Anisakis allergy debate: does an evolutionary approach help?
TL;DR: Allergic phenomena share common pathways with the immune response against helminth parasites, as well as putatively crossreacting antibodies, as are used in food allergy, depend on the clinical relevance of specific IgE and deserve careful interpretation in the various forms of A. simplex.
Journal ArticleDOI
Effects of anisakid nematodes Anisakis simplex (s.l.), Pseudoterranova decipiens (s.l.) and Contracaecum osculatum (s.l.) on fish and consumer health ☆
Kurt Buchmann,Foojan Mehrdana +1 more
TL;DR: Clinical symptoms associated with the infection, termed anisakidosis, vary from irritation of the oesophagus and stomach, via nausea, vomiting and diarrhoea to severe epigastric and abdominal pain.
References
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Journal ArticleDOI
Genetic markers in the study of Anisakis typica (Diesing, 1860): larval identification and genetic relationships with other species of Anisakis Dujardin, 1845 (Nematoda: Anisakidae).
Simonetta Mattiucci,Lia Paggi,Giuseppe Nascetti,C. Portes Santos,Graça Costa,A. P. M. Di Beneditto,Renata Maria Arruda Ramos,M. Argyrou,R. Cianchi,Luciano Bullini +9 more
TL;DR: The present findings represent the first established records of intermediate/paratenic hosts of Anisakis typica and extend its range to Somali waters of the Indian Ocean and to the Eastern Mediterranean Sea.
Journal ArticleDOI
Molecular characterization of larval anisakid nematodes from marine fishes of madeira by a pcr-based approach, with evidence for a new species
TL;DR: One-hundred and fifteen anisakid larvae from 3 different fish hosts, Aphanopus carbo, Scomber japonicus, and Trachurus picturatus, caught in Madeiran waters, were identified by PCR-RFLP and an apparently undescribed taxon seems to be more similar to A. ziphidarum than to other species of the genus.
Journal ArticleDOI
Evidence for a new species of Anisakis Dujardin, 1845: morphological description and genetic relationships between congeners (Nematoda: Anisakidae).
Simonetta Mattiucci,Giuseppe Nascetti,Murray D. Dailey,Stephen C. Webb,Nélio B. Barros,R. Cianchi,Luciano Bullini +6 more
TL;DR: A low genetic identity was detected at allozyme level between the eight Anisakis species and was supported by differences in adult and larval morphology, as well as with respect to their main definitive hosts.
Journal ArticleDOI
Anisakis nascettii n. sp. (Nematoda: Anisakidae) from beaked whales of the southern hemisphere: morphological description, genetic relationships between congeners and ecological data
TL;DR: Morphological and morphological data indicate that the new species belongs to the ‘ziphidarum-group’; however, it is genetically very distinct from A. ziphidareum, and the parallelism between the clade formed by these two anisakine taxa further supports the hypothesis of host–parasite co-evolutionary relationships.
Journal ArticleDOI
Occurrence and molecular identification of Anisakis spp. from the North African coasts of Mediterranean Sea.
Sarra Farjallah,Badreddine Ben Slimane,M. Busi,Lia Paggi,Nabil Amor,Hager Blel,Khaled Said,Stefano D'Amelio +7 more
TL;DR: Polymerase chain reaction–restriction fragment length polymorphism analysis was used to investigate the occurrence of larval forms of different Anisakis species in teleost fishes and squid from North African coasts of the Mediterranean Sea and to establish the geographical and host range of these parasites in this area.
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