First molecular identification of the zoonotic parasite Anisakis pegreffii(Nematoda: Anisakidae) in a paraffin-embedded granuloma taken from a case of human intestinal anisakiasis in Italy
Simonetta Mattiucci,Michela Paoletti,Michela Paoletti,Francesco Borrini,Massimo Palumbo,Raffaele Macarone Palmieri,Vincenzo Gomes,Alessandra Casati,Giuseppe Nascetti +8 more
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TLDR
This is the first instance of human intestinal anisakiasis diagnosed using PCR of DNA purified from a fixed eosinophilic granuloma embedded in paraffin, reinforcing the pathological significance of the species A. pegreffii to humans.Abstract:
Anisakiasis is an important fish-borne zoonosis provoked by larval stages of nematodes belonging to the genus Anisakis. The detection and identification of human infections is difficult. This is due to: a) the low specificity of the clinical features and symptomatology related to human infections; b) the paucity of diagnostic features of larvae found in granulomatous lesions characteristic of "invasive anisakiasis"; and c) the lack morphological characters diagnostic at the specific level when larvae of Anisakis are detected. Thus, molecular-based diagnostic approaches are warranted. We have developed a PCR method that amplifies the DNA of Anisakis spp. in fixed paraffin-embedded tissues. This method was applied to a granuloma removed from a human case of intestinal anisakiasis in Italy. Specific primers of the mtDNA cox2 gene were used and sequence analysis was performed according to the procedures already established for species of Anisakis. The sequence obtained (629 bp) was compared with those of the other species of Anisakis which have so far been genetically characterized and with sequences obtained from larval stages of Anisakis collected from the Mediterranean fish Engraulis encrasicolus. This enabled the genetic identification of the larva in the human tissue as A. pegreffii. This is the first instance of human intestinal anisakiasis diagnosed using PCR of DNA purified from a fixed eosinophilic granuloma embedded in paraffin. The case of human anisakiasis presented reinforces the pathological significance of the species A. pegreffii to humans. The molecular/genetic methodological approach based on mtDNA cox2 sequence analysis, described here, can allow easy and rapid identification of Anisakis spp. in formalin-fixed and paraffin embedded tissues removed from cases of either gastric or intestinal human anisakiasis.read more
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Foodborne anisakiasis and allergy.
TL;DR: Some aspects of this food-borne disease are reviewed and its link to chronic, allergic conditions in humans is explained and its subsequent sensitisation of the human patient is explained.
Journal ArticleDOI
Larval migration of the zoonotic parasite Anisakis pegreffii (Nematoda: Anisakidae) in European anchovy, Engraulis encrasicolus: Implications to seafood safety
Paolo Cipriani,Paolo Cipriani,Virginia Acerra,Virginia Acerra,Bruno Bellisario,Gian Luca Sbaraglia,Roberto Cheleschi,Giuseppe Nascetti,Simonetta Mattiucci +8 more
TL;DR: Temperature plays an important role in the post-mortem motility of A. pegreffii larvae in anchovies, and the importance of the adoption of rules to prevent human anisakiasis was underlined.
Journal ArticleDOI
A survey of zoonotic nematodes of commercial key fish species from major European fishing grounds—Introducing the FP7 PARASITE exposure assessment study
Arne Levsen,Cecilie Smith Svanevik,Paolo Cipriani,Paolo Cipriani,Simonetta Mattiucci,Simonetta Mattiucci,Lee C. Hastie,Ivana Bušelić,Ivona Mladineo,Horst Karl,Ute Ostermeyer,Kurt Buchmann,Danjal P. Højgaard,Ángel F. González,Santiago Pascual,Graham J. Pierce,Graham J. Pierce,Graham J. Pierce +17 more
TL;DR: The epidemiological survey was carried out to provide the basis for analysis and prediction of consumer exposure risk due to the presence of anisakid nematodes in fish from European wild-catch fisheries, and represents the largest and most comprehensive epidemiological data compilation of an isakids ever generated.
Journal ArticleDOI
Human anisakiasis in Italy: a retrospective epidemiological study over two decades.
Lisa Guardone,Andrea Armani,Daniele Nucera,Francesco Costanzo,Simonetta Mattiucci,Fabrizio Bruschi +5 more
TL;DR: Attention should be given to the history, in particular when raw marinated anchovies, proven to be the main source of human anisakiasis in Italy, are consumed, in order to assess correct epidemiological data, a confirmed and specific etiological identification should always be sought.
Journal ArticleDOI
Genetic identification and distribution of the parasitic larvae of Anisakis pegreffii and Anisakis simplex (s. s.) in European hake Merluccius merluccius from the Tyrrhenian Sea and Spanish Atlantic coast: implications for food safety.
Paolo Cipriani,Giorgio Smaldone,Virginia Acerra,Luisa D'Angelo,Aniello Anastasio,Bruno Bellisario,Giuseppe Palma,Giuseppe Nascetti,Simonetta Mattiucci +8 more
TL;DR: Given that the pathogenic role in relation to man is known for these two species of Anisakis, both the flesh inspection and the infection rates of the different anisakid species assume particular importance in terms of assessing the risk they pose to humans.
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