First molecular identification of the zoonotic parasite Anisakis pegreffii(Nematoda: Anisakidae) in a paraffin-embedded granuloma taken from a case of human intestinal anisakiasis in Italy
Simonetta Mattiucci,Michela Paoletti,Michela Paoletti,Francesco Borrini,Massimo Palumbo,Raffaele Macarone Palmieri,Vincenzo Gomes,Alessandra Casati,Giuseppe Nascetti +8 more
Reads0
Chats0
TLDR
This is the first instance of human intestinal anisakiasis diagnosed using PCR of DNA purified from a fixed eosinophilic granuloma embedded in paraffin, reinforcing the pathological significance of the species A. pegreffii to humans.Abstract:
Anisakiasis is an important fish-borne zoonosis provoked by larval stages of nematodes belonging to the genus Anisakis. The detection and identification of human infections is difficult. This is due to: a) the low specificity of the clinical features and symptomatology related to human infections; b) the paucity of diagnostic features of larvae found in granulomatous lesions characteristic of "invasive anisakiasis"; and c) the lack morphological characters diagnostic at the specific level when larvae of Anisakis are detected. Thus, molecular-based diagnostic approaches are warranted. We have developed a PCR method that amplifies the DNA of Anisakis spp. in fixed paraffin-embedded tissues. This method was applied to a granuloma removed from a human case of intestinal anisakiasis in Italy. Specific primers of the mtDNA cox2 gene were used and sequence analysis was performed according to the procedures already established for species of Anisakis. The sequence obtained (629 bp) was compared with those of the other species of Anisakis which have so far been genetically characterized and with sequences obtained from larval stages of Anisakis collected from the Mediterranean fish Engraulis encrasicolus. This enabled the genetic identification of the larva in the human tissue as A. pegreffii. This is the first instance of human intestinal anisakiasis diagnosed using PCR of DNA purified from a fixed eosinophilic granuloma embedded in paraffin. The case of human anisakiasis presented reinforces the pathological significance of the species A. pegreffii to humans. The molecular/genetic methodological approach based on mtDNA cox2 sequence analysis, described here, can allow easy and rapid identification of Anisakis spp. in formalin-fixed and paraffin embedded tissues removed from cases of either gastric or intestinal human anisakiasis.read more
Citations
More filters
Journal ArticleDOI
Gene expression profiles of antigenic proteins of third stage larvae of the zoonotic nematode Anisakis pegreffii in response to temperature conditions.
Marialetizia Palomba,Michela Paoletti,Alessandra Colantoni,Aurelia Rughetti,Giuseppe Nascetti,Simonetta Mattiucci +5 more
TL;DR: The results suggest that temperature conditions do regulate the gene expression profiles of A. pegreffii larvae, and regulation of the glycoprotein A.peg-7 is likely to be related to other factors such as the host’s immune response.
Journal ArticleDOI
Detection of ascaridoid nematode parasites in the important marine food-fish Conger myriaster (Brevoort) (Anguilliformes: Congridae) from the Zhoushan Fishery, China.
TL;DR: This study represents the first report of C. myriaster from the Zhoushan Fishery being heavily infected with third-stage ascaridoid larvae, and suggests a high risk of anisakidosis or associated allergies for people consuming raw or poorly cooked fish originating from this marine area.
Journal ArticleDOI
Mitochondrial genomes of Anisakis simplex and Contracaecum osculatum (sensu stricto)--comparisons with selected nematodes.
Namitha Mohandas,Abdul Jabbar,Magdalena Podolska,Xing-Quan Zhu,D. Timothy J. Littlewood,Aaron R. Jex,Robin B. Gasser +6 more
TL;DR: These mt genomes provide a stepping-stone for future comparative analyses of a range of anisakids and a basis for reinvestigating their genetic relationships, and might be used in prospecting for cryptic species and exploring host affiliations.
Journal ArticleDOI
Anisakid and raphidascaridid nematodes (Ascaridoidea) infection in the important marine food-fish Lophius litulon (Jordan) (Lophiiformes: Lophiidae)
TL;DR: The present results provided important information for determining the composition of the nematode parasite fauna of L. litulon in the East China Sea, and it is also a key step for the risk assessment of human anisakidosis when L.litulon caught from theEast China Sea are consumed.
Journal ArticleDOI
Role of biogenic amines in the post-mortem migration of Anisakis pegreffii (Nematoda: Anisakidae Dujardin, 1845) larvae into fish fillets.
TL;DR: Measured pH and biogenic amine profile during storage indicated that certain biochemical conditions trigger larval migration into fillets, and cadaverine and putrescine levels correlated the most with the post-mortem migration at 4°C, while tyramine levels were significant at both temperatures.
References
More filters
Journal ArticleDOI
Confidence limits on phylogenies: an approach using the bootstrap.
TL;DR: The recently‐developed statistical method known as the “bootstrap” can be used to place confidence intervals on phylogenies and shows significant evidence for a group if it is defined by three or more characters.
Journal ArticleDOI
The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools.
TL;DR: ClUSTAL X is a new windows interface for the widely-used progressive multiple sequence alignment program CLUSTAL W, providing an integrated system for performing multiple sequence and profile alignments and analysing the results.
Journal ArticleDOI
Bioedit: a user-friendly biological sequence alignment editor and analysis program for windows 95/98/ nt
Journal ArticleDOI
MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) Software Version 4.0
TL;DR: Version 4 of MEGA software expands on the existing facilities for editing DNA sequence data from autosequencers, mining Web-databases, performing automatic and manual sequence alignment, analyzing sequence alignments to estimate evolutionary distances, inferring phylogenetic trees, and testing evolutionary hypotheses.
Journal ArticleDOI
An Empirical Test of Bootstrapping as a Method for Assessing Confidence in Phylogenetic Analysis
David M. Hillis,James J. Bull +1 more
TL;DR: This work uses computer simulations and a laboratory-generated phylogeny to test bootstrapping results of parsimony analyses, and indicates that any given bootstrap proportion provides an unbiased but highly imprecise measure of repeatability, unless the actual probability of replicating the relevant result is nearly one.
Related Papers (5)
Advances and trends in the molecular systematics of anisakid nematodes, with implications for their evolutionary ecology and host-parasite co-evolutionary processes.
Anisakis simplex: from Obscure Infectious Worm to Inducer of Immune Hypersensitivity
Audicana Mt,Kennedy Mw +1 more