First molecular identification of the zoonotic parasite Anisakis pegreffii(Nematoda: Anisakidae) in a paraffin-embedded granuloma taken from a case of human intestinal anisakiasis in Italy
Simonetta Mattiucci,Michela Paoletti,Michela Paoletti,Francesco Borrini,Massimo Palumbo,Raffaele Macarone Palmieri,Vincenzo Gomes,Alessandra Casati,Giuseppe Nascetti +8 more
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TLDR
This is the first instance of human intestinal anisakiasis diagnosed using PCR of DNA purified from a fixed eosinophilic granuloma embedded in paraffin, reinforcing the pathological significance of the species A. pegreffii to humans.Abstract:
Anisakiasis is an important fish-borne zoonosis provoked by larval stages of nematodes belonging to the genus Anisakis. The detection and identification of human infections is difficult. This is due to: a) the low specificity of the clinical features and symptomatology related to human infections; b) the paucity of diagnostic features of larvae found in granulomatous lesions characteristic of "invasive anisakiasis"; and c) the lack morphological characters diagnostic at the specific level when larvae of Anisakis are detected. Thus, molecular-based diagnostic approaches are warranted. We have developed a PCR method that amplifies the DNA of Anisakis spp. in fixed paraffin-embedded tissues. This method was applied to a granuloma removed from a human case of intestinal anisakiasis in Italy. Specific primers of the mtDNA cox2 gene were used and sequence analysis was performed according to the procedures already established for species of Anisakis. The sequence obtained (629 bp) was compared with those of the other species of Anisakis which have so far been genetically characterized and with sequences obtained from larval stages of Anisakis collected from the Mediterranean fish Engraulis encrasicolus. This enabled the genetic identification of the larva in the human tissue as A. pegreffii. This is the first instance of human intestinal anisakiasis diagnosed using PCR of DNA purified from a fixed eosinophilic granuloma embedded in paraffin. The case of human anisakiasis presented reinforces the pathological significance of the species A. pegreffii to humans. The molecular/genetic methodological approach based on mtDNA cox2 sequence analysis, described here, can allow easy and rapid identification of Anisakis spp. in formalin-fixed and paraffin embedded tissues removed from cases of either gastric or intestinal human anisakiasis.read more
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First Record of Anisakis simplex Third-Stage Larvae (Nematoda, Anisakidae) in European Hake Merluccius merluccius lessepsianus in Egyptian Water
TL;DR: The present study was considered as the first report of anisakid larvae from European hake in the Egyptian water and a positive relationship between host length/weight and prevalence of infection was found.
Journal ArticleDOI
Mutation scanning-based analysis of anisakid larvae from Sillago flindersi from Bass Strait, Australia
Abdul Jabbar,Avalene T. W. Khoon,Teo X. Hui,Bjoern C. Schaeffner,Aaron R. Jex,Matthew J. Nolan,Andreas L. Lopata,Robin B. Gasser,Ian Beveridge +8 more
TL;DR: The PCR‐coupled mutation scanning approach employed herein is an effective tool for the genetic characterisation of anisakid nematodes for diagnostic and analytical purposes.
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Anisakis pegreffii impacts differentiation and function of human dendritic cells.
Chiara Napoletano,Simonetta Mattiucci,Alessandra Colantoni,Federico Battisti,Ilaria Grazia Zizzari,Hassan Rahimi,Marianna Nuti,Aurelia Rughetti +7 more
TL;DR: Human dendritic cells show remarkable phenotypic changes when matured in the presence of helminth‐derived products, which suggest a dynamic contribution of DCs to the induction and maintenance of the inflammatory response against A. pegreffii, and are suggested to contribute to immunological tolerance against these pathogens.
Journal ArticleDOI
Epidemiology and management of foodborne nematodiasis in the European Union, systematic review 2000–2016
TL;DR: A review of available data on epidemiology and management of foodborne nematodiasis in the European Union, detect any trends and determine the possible causes of the observed changes to determine the greatest health risk.
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Peppermint (Mentha piperita) and albendazole against anisakiasis in an animal model
TL;DR: The aim of this study was to determine the efficacy of peppermint essential oil and its main components against the parasite in comparison to albendazole, a drug currently prescribed to treat anisakiasis.
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