Journal ArticleDOI
Fluorescent Benzothiadiazole Derivatives as Fluorescence Imaging Dyes: A Decade of New Generation Probes.
TLDR
A review of the use of fluorescent 2,1,3-benzothiadiazole (BTD) derivatives in bioimaging is presented in this paper, where the authors describe the application of BTD-containing fluorophores applied as, inter alia, bioprobes for imaging cell nuclei, mitochondria, lipid droplets, sensors, markers for proteins and related events, biological processes and activities, lysosomes, plasma membranes, multicellular models and animals.Abstract:
The current review describes advances in the use of fluorescent 2,1,3-benzothiadiazole (BTD) derivatives after nearly one decade since the first description of bioimaging experiments using this class of fluorogenic dyes. The review describes the use of BTD-containing fluorophores applied as, inter alia, bioprobes for imaging cell nuclei, mitochondria, lipid droplets, sensors, markers for proteins and related events, biological processes and activities, lysosomes, plasma membranes, multicellular models, and animals. A number of physicochemical and photophysical properties commonly observed for BTD fluorogenic structures are also described.read more
Citations
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Journal ArticleDOI
Dithiol‐Activated Bioorthogonal Chemistry for Endoplasmic Reticulum‐Targeted Synergistic Chemophototherapy
TL;DR: In this article , a bioorthogonal system was established to target the endoplasmic reticulum (ER) of liver cancer HepG2 cells using benzothiadiazole-based organic nitrite donors that are stable against biorelevant species but selectively respond to dithiol species through SNAr/intramolecular cyclization tandem reactions in the aqueous media.
Journal ArticleDOI
Small Fluorogenic Amino Acids for Peptide‐Guided Background‐Free Imaging
Fabio De Moliner,Ż. Konieczna,Lorena Mendive-Tapia,Rebecca Sonia Saleeb,Katie Morris,Juan A. González-Vera,Takeshi Kaizuka,Seth G. N. Grant,Mathew H. Horrocks,Marc Vendrell +9 more
TL;DR: In this paper , a library of benzodiazole amino acids was built for background-free fluorescence microscopy and used for solid-phase synthesis of fluorescent peptides, which was shown to retain the binding capabilities of bioactive peptides and display excellent signal-to-background ratios.
Journal ArticleDOI
Dithiol-Activated Bioorthogonal Chemistry for Endoplasmic Re-ticulum-Targeted Synergistic Chemophototherapy.
TL;DR: In this paper , a bioorthogonal click and release system was established to target the endoplasmic reticulum (ER) of liver cancer HepG2 cells, which was achieved by exogenous β-galactosamine-modified dithiols, leading to production of fluorogenic photosensitizer and release of nitrite and nonivamide (a TRPV1 agonist).
Journal ArticleDOI
Molecular Environment Effects That Modulate the Photophysical Properties of Novel 1,3-Phosphinoamines Based on 2,1,3-Benzothiadiazole
TL;DR: In this article , the synthesis, crystal structure, and photophysical properties of novel 1,3-phosphinoamines based on 4-amino-2,1, 3-benzothiadiazole (NH2-btd) were reported.
Journal ArticleDOI
Bis-triazolylchalcogenium-Functionalized Benzothiadiazole Derivatives as Light-up Sensors for DNA and BSA.
Roberta Krüger,Allya Larroza,Mariana G. Fronza,Isadora Tisoco,Lucielli Savegnago,Joel S. Reis,Davi F. Back,Bernardo A. Iglesias,Diego Alves +8 more
TL;DR: A range of bis-triazolylchalcogenium-BTD 3a-3h was synthesized by a copper-catalyzed azide-alkyne cycloaddition of azido aryl-cogenides 1 and 4,7-diethynylbenzo[c][1,2,5]thiadiazole 2 as mentioned in this paper.
References
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TL;DR: The focus is on protein detection in live versus fixed cells: determination of protein expression, localization, activity state, and the possibility for combination of fluorescent light microscopy with electron microscopy.
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James A. Olzmann,Pedro Carvalho +1 more
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Excited-state intramolecular proton-transfer (ESIPT) based fluorescence sensors and imaging agents
Adam C. Sedgwick,Luling Wu,Hai Hao Han,Steven D. Bull,Xiao-Peng He,Tony D. James,Tony D. James,Jonathan L. Sessler,Ben Zhong Tang,He Tian,Juyoung Yoon +10 more
TL;DR: This review will explore recent advances in the design and application of excited-state intramolecular proton-transfer (ESIPT) based fluorescent probes.