Formin homology domains of Daam1 bind to Fascin and collaboratively promote pseudopodia formation and cell migration in breast cancer.
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Wang et al. as discussed by the authors found that dishevelled-associated activator of morphogenesis 1 (Daam1) regulates invadopodia extension and BrCa cell motility.Abstract:
Objectives Cancer cell migration to secondary organs remains an essential cause of death among breast cancer (BrCa) patients. Cell motility mainly relies on actin dynamics. Our previous reports verified that dishevelled-associated activator of morphogenesis 1 (Daam1) regulates invadopodia extension and BrCa cell motility. However, how Daam1 is involved in actin filament assembly and promotes pseudopodia formation in BrCa cells remains unclear. Materials and methods One hundred human BrCa samples were collected at Women's Hospital of Nanjing Medical University. Immunohistochemistry (IHC) was used to examine Daam1 and Fascin expression. Wound healing and Boyden chamber assays were used to explore cell migration and pseudopodia extension of BrCa cells. Co-IP/pull down and Western blotting were performed to study the physical interaction between Daam1 and Fascin. Immunofluorescence assays were performed to observe whether Daam1 and Fascin were colocalized and mediated actin filament assembly. Results Fascin was upregulated in BrCa tissues compared with that in paracarcinoma tissues. The downregulation of Fascin caused a decline in pseudopodia formation and cell motility. Moreover, we found that Daam1 interacted with Fascin via formin homology (FH) domains, especially the FH2 domain. Immunofluorescence assays showed that Daam1 and Fascin partially colocalized to actin filaments, and the knockdown of Daam1 or Fascin failed to colocalize to short and curved actin filaments. Conclusions Daam1 specifically binds to Fascin via FH domains and cooperatively facilitates pseudopodia formation and cell migration by promoting actin filament assembly in BrCa.read more
Citations
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YWHAZ interacts with DAAM1 to promote cell migration in breast cancer
TL;DR: In this article, the expression levels of DAAM and YWHAZ were examined by immunohistochemistry (IHC) staining in breast cancer (BrCa) tissues.
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Formins in Human Disease.
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AKT Serine/Threonine Kinase 2-mediated phosphorylation of Fascin Threonine 403 regulates esophageal cancer progression.
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Preliminary Investigation on the Involvement of Cytoskeleton-Related Proteins, DAAM1 and PREP, in Human Testicular Disorders
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Formin protein DIAPH1 positively regulates PD-L1 expression and predicts the therapeutic response to anti-PD-1/PD-L1 immunotherapy.
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TL;DR: Wang et al. as mentioned in this paper explored the expressions, prognostic values, and immunological correlations of Formins in cancer and found that specific Formins were dysregulated and immuno-biologically correlated in breast cancer (BRCA).
References
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Journal ArticleDOI
Filopodia: molecular architecture and cellular functions
TL;DR: The increased understanding of the functions of various actin-associated proteins during the initiation and elongation of filopodia has provided new information on the mechanisms of filipodia formation in distinct cell types.
Journal ArticleDOI
Wnt/Frizzled Activation of Rho Regulates Vertebrate Gastrulation and Requires a Novel Formin Homology Protein Daam1
Raymond Habas,Yoichi Kato,Xi He +2 more
TL;DR: It is reported that in human cells and during Xenopus embryogenesis, Wnt/Fz signaling activates the small GTPase Rho, a key regulator of cytoskeleton architecture.
Journal ArticleDOI
Role of fascin in filopodial protrusion
Danijela Matic Vignjevic,Shin Ichiro Kojima,Yvonne S. Aratyn,Oana Danciu,Tatyana Svitkina,Gary G. Borisy +5 more
TL;DR: It is proposed that fascin is a key specific actin cross-linker, providing stiffness for filopodial bundles, and that its dynamic behavior allows for efficient coordination between elongation and bundling of filopODial actin filaments.
Journal ArticleDOI
Cell motility in cancer invasion and metastasis: insights from simple model organisms
TL;DR: In this Review, insights relevant to human cancer that are derived from the study of cell motility in non-mammalian model organisms are evaluated, including the detailed signalling network that governs chemotaxis towards chemokines.
Journal ArticleDOI
Mechanism of actin filament bundling by fascin.
Silvia Jansen,Agnieszka Collins,Changsong Yang,Grzegorz Rebowski,Tatyana Svitkina,Roberto Dominguez +5 more
TL;DR: It is shown that fascin contains two major actin-binding sites, coinciding with regions of high sequence conservation in β-trefoil domains 1 and 3, which could guide the rational design of new anticancer fascin inhibitors.