Journal ArticleDOI
Functional analysis of CsCBF3 transcription factor in tea plant ( Camellia sinensis ) under cold stress
Ying Yin,Qingping Ma,Zi-xuan Zhu,Qiaoyun Cui,Changsong Chen,Xuan Chen,Wanping Fang,Xinghui Li +7 more
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TLDR
It was observed that the CsCBF3 gene regulates the expressions of downstream genes of cold responsive pathway, such as AtCOR15a and AtCOR78, and provide potential applications in molecular breeding to enhance cold tolerance in tea plant.Abstract:
C-repeat binding factors (CBFs) are involved in multiple pathways of plant growth, development and stress responses. In this study, CsCBF3, a gene encoding a polypeptide of 274 amino acids and containing the structural feature of AP2 domain in CBF protein family, was characterized from tea plant [Camellia sinensis (L.) O. Kuntze]. CsCBF3 was located in cell nucleus and cytoplasm by subcellular localization analysis. Expression analysis revealed that CsCBF3 was induced by low temperature stress, abscisic acid and drought treatment in tea leaves. CsCBF3 overexpressed Arabidopsis displayed higher tolerance to cold stress and improved photosynthesis ability with less damage under cold condition compared to wild type plants. Furthermore, it was observed that the CsCBF3 gene regulates the expressions of downstream genes of cold responsive pathway, such as AtCOR15a and AtCOR78. These results indicate that CsCBF3 plays an important role in responding to cold stress and provide potential applications in molecular breeding to enhance cold tolerance in tea plant.read more
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Exogenous Melatonin Alleviates Cold Stress by Promoting Antioxidant Defense and Redox Homeostasis in Camellia sinensis L.
TL;DR: This study provides strong evidence that melatonin could alleviate cold-induced adverse effects in tea plants by reducing oxidative stress through enhanced antioxidant potential and redox homeostasis.
Journal ArticleDOI
Sesquiterpene glucosylation mediated by glucosyltransferase UGT91Q2 is involved in the modulation of cold stress tolerance in tea plants.
Mingyue Zhao,Na Zhang,Ting Gao,Jieyang Jin,Tingting Jing,Jingming Wang,Yi Wu,Xiaochun Wan,Wilfried Schwab,Wilfried Schwab,Chuankui Song +10 more
TL;DR: The first plant UGT (UGT91Q2) in tea plant is discovered, whose expression is strongly induced by cold stress and which specifically catalyzes the glucosylation of nerolidol, revealing previously unidentified roles of volatiles in response to abiotic stress in plants.
Journal ArticleDOI
SmMYB36, a Novel R2R3-MYB Transcription Factor, Enhances Tanshinone Accumulation and Decreases Phenolic Acid Content in Salvia miltiorrhiza Hairy Roots.
TL;DR: The wide distribution of MYB binding elements and electrophoretic mobility shift assay results indicated that SmMYB36 may be an effective tool to regulate metabolic flux shifts.
Journal ArticleDOI
Circular RNA architecture and differentiation during leaf bud to young leaf development in tea ( Camellia sinensis )
Wei Tong,Jie Yu,Yan Hou,Fangdong Li,Qiying Zhou,Qiying Zhou,Chaoling Wei,Jeffrey L. Bennetzen,Jeffrey L. Bennetzen +8 more
TL;DR: This study undertook a systematic identification of circRNAs from different stage tissues of tea plant (Camellia sinensis) leaf development using rRNA-depleted circular RNA-seq and found that circRNA abundances were positively correlated with the mRNA transcript abundances of their parental genes.
Journal ArticleDOI
Glucosyltransferase CsUGT78A14 Regulates Flavonols Accumulation and Reactive Oxygen Species Scavenging in Response to Cold Stress in Camellia sinensis.
Mingyue Zhao,Jieyang Jin,Ting Gao,Na Zhang,Tingting Jing,Jingming Wang,Qiuyan Ban,Wilfried Schwab,Wilfried Schwab,Chuankui Song +9 more
TL;DR: It is demonstrated that CsUGT78A14 plays a critical role in cold stress by increasing flavonols accumulation and ROS scavenging capacity, providing novel insights into the biological role of UGTs and flavonoids in plants.
References
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PLANT COLD ACCLIMATION: Freezing Tolerance Genes and Regulatory Mechanisms
TL;DR: This review of recent advances in determining the nature and function of genes with roles in freezing tolerance and the mechanisms involved in low temperature gene regulation and signal transduction concludes that cold acclimation includes the expression of certain cold-induced genes that function to stabilize membranes against freeze-induced injury.
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