Journal ArticleDOI
Functionalization of mesoporous silica for lipase immobilization: Characterization of the support and the catalysts
Rosa M. Blanco,Pilar Terreros,Mónica Fernández-Pérez,Cristina Otero,Guadalupe Dı́az-González +4 more
TLDR
In this paper, a support for enzyme immobilization was prepared by functionalization of mesoporous silica with octyltriethoxysilane, which enabled the adsorption of lipase from Candida antarctica B via strong hydrophobic interactions.Abstract:
A support for enzyme immobilization was prepared by functionalization of mesoporous silica with octyltriethoxysilane. The features of the surface enables the adsorption of lipase from Candida antarctica B via strong hydrophobic interactions, enhancing the stability of the adsorbed enzyme molecules. Derivatives with a high enzyme loading (200 mg protein/g of silica) can be obtained due to the high porosity and surface properties of the support while the immobilization occurs in a monolayer fashion. The lack of inactive enzyme aggregates, together with the high enzyme loading, are responsible for the high catalytic activity achieved by these species. Derivatives were prepared with different lipase loading, and the activities were tested and compared to the commercial derivative Novozym 435. The stability of the catalyst and hence its industrial applicability were tested by performing subsequent reaction cycles of acylation of ethanolamine with lauric acid in acetonitrile. Conversion was quantitative even after 15 reaction cycles.read more
Citations
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Nanostructures for enzyme stabilization
TL;DR: Recently reported approaches to improve the enzyme stability in various nanostructures such as nanoparticles, nanofibers, mesoporous materials, and single enzyme nanoparticles (SENs) are reviewed.
Journal ArticleDOI
Immobilisation and application of lipases in organic media.
TL;DR: The practical applications of lipases in organic media reviewed include ester synthesis, modification of triacylglycerols and phospholipids, fatty acid enrichment, enantiomer resolution, biodiesel production and acylation of carbohydrates and bioactive compounds.
Journal ArticleDOI
Enzyme immobilization: an update
TL;DR: This paper is a review of the recent literatures on enzyme immobilization by various techniques, the need for immobilization and different applications in industry, covering the last two decades.
Journal ArticleDOI
Proteins in mesoporous silicates.
TL;DR: This Review discusses the application of ordered, large-pore, functionalized mesoporous silicates to immobilize proteins for biocatalysis.
Journal ArticleDOI
Immobilised enzymes: science or art?
TL;DR: Combinatorial approaches are increasingly applied in the design of robust immobilised enzymes by rational combination of fundamental immobilisation techniques or with other relevant technologies to solve specific problems that cannot be solved by one of these basic immobilised techniques.
References
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Journal ArticleDOI
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products.
Journal Article
Cleavage of structural proteins during the assemble of the head of bacterio-phage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products as mentioned in this paper.
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On a Theory of the van der Waals Adsorption of Gases
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The sequence, crystal structure determination and refinement of two crystal forms of lipase B from Candida antarctica.
TL;DR: The structure of Candida antarctica lipase B shows that the enzyme has a Ser-His-Asp catalytic triad in its active site, which accounts for the substrate specificity and high degree of stereospecificity of this lipase.