Journal ArticleDOI
Genetic variation in the human insulin gene
TLDR
Four recombinant lambda phages containing nucleotide sequences complementary to a cloned human preproinsulin DNA probe have been isolated from human DNA and restriction analyses suggest that these two gene types are allelic variants of a single insulin gene.Abstract:
Four recombinant lambda phages containing nucleotide sequences complementary to a cloned human preproinsulin DNA probe have been isolated from human DNA. Restriction analyses in conjunction with Southern hybridizations reveal two types of gene sequences. One isolate of each type was subjected to complete nucleotide sequence determination. The sequences contain the entire preproinsulin messenger RNA region, two intervening sequence. 260 nucleotides upstream from the messenger RNA capping site, and 35 nucleotides beyond the polyadenylate attachment site. Our results strongly suggest that these two gene types are allelic variants of a single insulin gene.read more
Citations
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Insulin and insulin-like growth factor signalling in neoplasia
TL;DR: Epidermiological, clinical and laboratory research methods are being used to investigate novel cancer prevention and treatment strategies related to insulin and IGF signalling, including the use of novel receptor-specific antibodies, receptor kinase inhibitors and AMP-activated protein kinase activators.
Journal ArticleDOI
Heritable formation of pancreatic β -cell tumours in transgenic mice expressing recombinant insulin/simian virus 40 oncogenes
TL;DR: Following the transfer into fertilized mouse eggs of recombinant genes composed of the upstream region of the rat insulin II gene linked to sequences coding for the large-T antigen of simian virus 40, large- T antigen is detected exclusively in the β-Cells of the endocrine pancreas of transgenic mice.
Journal ArticleDOI
A polymorphic locus near the human insulin gene is associated with insulin-dependent diabetes mellitus.
TL;DR: The genotype at this locus of 393 unrelated diabetic and nondiabetic individuals is determined and differences were observed in the genotypie and allelic frequencies between groups of different races.
Journal ArticleDOI
Cell-specific expression controlled by the 5′-flanking region of insulin and chymotrypsin genes
TL;DR: DNA sequences containing the 5′-flanking regions of the insulin and chymotrypsin genes were linked to the coding sequence of the chloramphenicol acetyltransferase (CAT) gene, which elicits preferential expression of CAT activity when introduced into cells producing insulin.
Journal ArticleDOI
Restriction sites containing CpG show a higher frequency of polymorphism in human DNA
TL;DR: The occurrence of significant variation in Msp I and Taq I sites supports the view that methylated cytosine residues are hotspots for mutation in mammalian DNA.
References
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Journal ArticleDOI
Detection of specific sequences among DNA fragments separated by gel electrophoresis.
TL;DR: This paper describes a method of transferring fragments of DNA from agarose gels to cellulose nitrate filters that can be hybridized to radioactive RNA and hybrids detected by radioautography or fluorography.
Journal ArticleDOI
A new method for sequencing DNA
Allan M. Maxam,Walter Gilbert +1 more
TL;DR: Reactions that cleave DNA preferentially at guanines, at adenines,At cytosines and thymines equally, and at cytosine alone are described.
Journal ArticleDOI
The isolation of structural genes from libraries of eucaryotic DNA
Tom Maniatis,Ross C. Hardison,Elizabeth Lacy,Joyce Lauer,Catherine O'Connell,Diana Quon,Gek Kee Sim,Argiris Efstratiadis +7 more
TL;DR: A procedure for eucaryotic structural gene isolation which involves the construction and screening of cloned libraries of genomic DNA is presented and Restriction mapping and hybridization studies reveal the presence of closely linked beta-globin genes.
Journal ArticleDOI
Changes in size and secondary structure of the ribosomal transcription unit during vertebrate evolution
TL;DR: Analysis of the double-stranded, S 1 -resistant fragments indicates that there is a direct relationship between the hairpin loops seen in the electron microscope and secondary structure in aqueous solution.
Journal ArticleDOI
Are snRNPs involved in splicing
TL;DR: Several lines of evidence are presented that suggest a direct involvement of snRNPs in the splicing of hnRNA molecules, including the observation that the nucleotide sequence at the 5′ end of U1 RNA exhibits extensive complementarity to those across splice junctions in hn RNA molecules.