Genetically Encoded CRISPR Components Yield Efficient Gene Editing in the Invasive Pest Drosophila suzukii.
Nikolay P. Kandul,Esther J. Belikoff,Junru Liu,Anna Buchman,Fang Li,Akihiko Yamamoto,Ting Yang,Isaiah Shriner,Maxwell J. Scott,Omar S. Akbari +9 more
- Vol. 4, Iss: 5, pp 739-751
TLDR
The Drosophila suzukii Matsumura is a global pest of economically important soft-skinned fruits and it is largely controlled through r... as mentioned in this paper, but it is difficult to control.Abstract:
Originally from Asia, Drosophila suzukii Matsumura is a global pest of economically important soft-skinned fruits. Also commonly known as spotted wing drosophila, it is largely controlled through r...read more
Citations
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Genetically engineered insects with sex-selection and genetic incompatibility enable population suppression
TL;DR: In this paper , a proof of concept by combining tetracycline-controlled female lethality constructs with a pyramus-targeting EGI line in the model insect Drosophila melanogaster is presented.
Journal ArticleDOI
Precision Guided Sterile Males Suppress Populations of an Invasive Crop Pest
Nikolay P. Kandul,Junru Liu,Anna Buchman,Isaiah Shriner,Rodrigo M. Corder,Natalie Rose Warsinger-Pepe,Ting Yang,Amarish Kumar Yadav,Maxwell J. Scott,John M. Marshall,Omar S. Akbari +10 more
TL;DR: In this article , the accuracy of programmable CRISPR gene targeting was used to generate sterilized males that can be deployed to suppress populations of Drosophila suzukii.
Journal ArticleDOI
Evaluation of Additional Drosophila suzukii Male-Only Strains Generated Through Remobilization of an FL19 Transgene
TL;DR: The aim of this study was to generate additional male-only strains that carried two copies of the FL19 transgene through crossing the original line with a piggyBac jumpstarter strain, and the most promising lines have been selected for future population suppression experiments with strains from different geographical locations.
Journal ArticleDOI
CRISPR/Cas9-based split homing gene drive targeting doublesex for population suppression of the global fruit pest Drosophila suzukii.
TL;DR: In this paper , a split CRISPR homing gene drive was used to suppress Drosophila suzukii, an invasive pest of soft-skinned fruits, by introducing the Dsx single guide RNA and DsRed genes.
References
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Journal ArticleDOI
RNA-Guided Human Genome Engineering via Cas9
Prashant Mali,Luhan Yang,Kevin M. Esvelt,John Aach,Marc Güell,James E. DiCarlo,Julie E. Norville,George M. Church,George M. Church +8 more
TL;DR: The type II bacterial CRISPR system is engineer to function with custom guide RNA (gRNA) in human cells to establish an RNA-guided editing tool for facile, robust, and multiplexable human genome engineering.
Journal ArticleDOI
Enzymatic assembly of DNA molecules up to several hundred kilobases
Daniel G. Gibson,Lei Young,Ray-Yuan Chuang,J. Craig Venter,Clyde A. Hutchison,Hamilton O. Smith +5 more
TL;DR: An isothermal, single-reaction method for assembling multiple overlapping DNA molecules by the concerted action of a 5′ exonuclease, a DNA polymerase and a DNA ligase is described.
Journal ArticleDOI
The Sublethal Effects of Pesticides on Beneficial Arthropods
TL;DR: The different types of sublethal effects on beneficial arthropods, focusing mainly on honey bees and natural enemies, are characterized, and the methods used in these studies are described.
Journal ArticleDOI
Possibilities of Insect Control or Eradication Through the Use of Sexually Sterile Males
TL;DR: The principles involved in controlling insects by releasing sexually sterile males among the existing natural population will be described and the potentialities as well as the limitations of the method will be discussed.
Journal ArticleDOI
Genome engineering of Drosophila with the CRISPR RNA-guided Cas9 nuclease
Scott J. Gratz,Alexander M. Cummings,Jennifer N. Nguyen,Danielle C. Hamm,Laura K. Donohue,Melissa M. Harrison,Jill Wildonger,Kate M. O'Connor-Giles +7 more
TL;DR: A bacterial CRISPR RNA/Cas9 system is adapted to precisely engineer the Drosophila genome and it is reported that Cas9-mediated genomic modifications are efficiently transmitted through the germline.