Journal ArticleDOI
GenomEra MRSA/SA, a fully automated homogeneous PCR assay for rapid detection of Staphylococcus aureus and the marker of methicillin resistance in various sample matrixes.
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TLDR
The GenomEra MRSA/SA assay provides rapid assistance for the detection of MRSA as well as invasive staphylococcal infections and helps the early targeting of antimicrobial therapy to patients with potential MRSA infection.Abstract:
The GenomEra MRSA/SA assay (Abacus Diagnostica, Turku, Finland) is the first commercial homogeneous PCR assay using thermally stable, intrinsically fluorescent time-resolved fluorometric (TRF) labels resistant to autofluorescence and other background effects. This fully automated closed tube PCR assay simultaneously detects Staphylococcus aureus specific DNA and the mecA gene within 50 min. It can be used for both screening and confirmation of methicillin-resistant and -sensitive S. aureus (MRSA and MSSA) directly in different specimen types or from preceding cultures. The assay has shown excellent performance in comparisons with other diagnostic methods in all the sample types tested. The GenomEra MRSA/SA assay provides rapid assistance for the detection of MRSA as well as invasive staphylococcal infections and helps the early targeting of antimicrobial therapy to patients with potential MRSA infection.read more
Citations
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Rapid Detection of Staphylococcus Aureus by Loop-Mediated Isothermal Amplification
TL;DR: The loop-mediated isothermal amplification (LAMP) method offers an alternative detection assay for nuc and mecA with a great advantage of the sensitivity and rapidity.
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The use of molecular methods for the detection and identification of methicillin-resistant Staphylococcus aureus
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Masako Mizusawa,Karen C. Carroll +1 more
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Métodos moleculares para el diagnóstico de septicemia
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References
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Journal ArticleDOI
Waves of resistance: Staphylococcus aureus in the antibiotic era
Henry F. Chambers,Frank R. DeLeo +1 more
TL;DR: The molecular epidemiology of the epidemic waves of peniillin- and methicillin-resistant strains of S. aureus that have occurred since 1940 are reviewed, with a focus on the clinical and molecular epidemiological of CA-MRSA.
Journal ArticleDOI
Community-associated methicillin-resistant Staphylococcus aureus: epidemiology and clinical consequences of an emerging epidemic.
Michael Z. David,Robert S. Daum +1 more
TL;DR: This review details the epidemiology of CA-MRSA strains and the clinical spectrum of infectious syndromes associated with them that ranges from a commensal state to severe, overwhelming infection and addresses the therapy of these infections and strategies for their prevention.
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Meticillin-resistant Staphylococcus aureus with a novel mecA homologue in human and bovine populations in the UK and Denmark: a descriptive study.
Laura García-Álvarez,Matthew T. G. Holden,Heather Lindsay,Cerian R. Webb,Derek F. J. Brown,Martin D. Curran,Enid Walpole,Karen Brooks,Derek Pickard,Christopher Teale,Julian Parkhill,Stephen D. Bentley,Giles Edwards,E Kirsty Girvan,Angela M. Kearns,Bruno Pichon,Robert Hill,Anders Rhod Larsen,Robert Skov,Sharon J. Peacock,Duncan J. Maskell,Mark A. Holmes +21 more
TL;DR: The discovery of a strain of S aureus isolated from bulk milk that was phenotypically resistant to meticillin but tested negative for the mecA gene is reported and new diagnostic guidelines for the detection of MRSA should consider the inclusion of tests for mecALGA251.
Journal ArticleDOI
Methicillin-Resistant Staphylococcus aureus (MRSA): Burden of disease and control challenges in Europe
Robin Köck,Karsten Becker,Barry Cookson,J.E.W.C. van Gemert-Pijnen,Stéphan Juergen Harbarth,Jan Kluytmans,Martin Mielke,Georg Peters,Robert Skov,Marc Struelens,Marc Struelens,Evelina Tacconelli,A Navarro Torné,Wolfgang Witte,Alexander W. Friedrich +14 more
TL;DR: The current burden of MRSA infections in healthcare and community settings across Europe is described and the main threats caused by recent changes in the epidemiology of MR SA are outlined, aimed at identifying unmet needs of surveillance, prevention and control ofMRSA in Europe.
Journal ArticleDOI
Global epidemiology of community-associated methicillin resistant Staphylococcus aureus (CA-MRSA).
TL;DR: The current molecular epidemiology of CA-MRSA is reviewed with respect to genetic diversity, global distribution, and factors related to its emergence and spread.