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Open AccessJournal ArticleDOI

GeoChip: a comprehensive microarray for investigating biogeochemical, ecological and environmental processes

TLDR
This is the first comprehensive microarray currently available for studying biogeochemical processes and functional activities of microbial communities important to human health, agriculture, energy, global climate change, ecosystem management, and environmental cleanup and restoration.
Abstract
Owing to their vast diversity and as-yet uncultivated status, detection, characterization and quantification of microorganisms in natural settings are very challenging, and linking microbial diversity to ecosystem processes and functions is even more difficult. Microarray-based genomic technology for detecting functional genes and processes has a great promise of overcoming such obstacles. Here, a novel comprehensive microarray, termed GeoChip, has been developed, containing 24 243 oligonucleotide (50 mer) probes and covering 410 000 genes in 4150 functional groups involved in nitrogen, carbon, sulfur and phosphorus cycling, metal reduction and resistance, and organic contaminant degradation. The developed GeoChip was successfully used for tracking the dynamics of metal-reducing bacteria and associated communities for an in situ bioremediation study. This is the first comprehensive microarray currently available for studying biogeochemical processes and functional activities of microbial communities important to human health, agriculture, energy, global climate change, ecosystem management, and environmental cleanup and restoration. It is particularly useful for providing direct linkages of microbial genes/populations to ecosystem processes and functions.

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Citations
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Journal ArticleDOI

The unseen majority: Soil microbes as drivers of plant diversity and productivity in terrestrial ecosystems

TL;DR: Overall, this review shows that soil microbes must be considered as important drivers of plant diversity and productivity in terrestrial ecosystems.
Journal ArticleDOI

The rhizosphere microbiome: significance of plant beneficial, plant pathogenic, and human pathogenic microorganisms

TL;DR: The main functions of rhizosphere microorganisms and how they impact on health and disease are reviewed and several strategies to redirect or reshape the rhizospheric microbiome in favor of microorganisms that are beneficial to plant growth and health are highlighted.
Journal ArticleDOI

Molecular ecological network analyses

TL;DR: A novel mathematical and bioinformatics framework to construct ecological association networks named molecular ecological networks (MENs) through Random Matrix Theory (RMT)-based methods is described, which provides powerful tools to elucidate network interactions in microbial communities and their responses to environmental changes.
Journal ArticleDOI

Stochastic Community Assembly: Does It Matter in Microbial Ecology?

TL;DR: Both stochastic and deterministic components embedded in various ecological processes, including selection, dispersal, diversification, and drift are described.
References
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Journal ArticleDOI

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TL;DR: A high-capacity system was developed to monitor the expression of many genes in parallel by means of simultaneous, two-color fluorescence hybridization, which enabled detection of rare transcripts in probe mixtures derived from 2 micrograms of total cellular messenger RNA.
Journal ArticleDOI

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TL;DR: Phylogenetic analysis of the retrieved rRNA sequence of an uncultured microorganism reveals its closest culturable relatives and may, together with information on the physicochemical conditions of its natural habitat, facilitate more directed cultivation attempts.
Journal ArticleDOI

Exploring the Metabolic and Genetic Control of Gene Expression on a Genomic Scale

TL;DR: DNA microarrays containing virtually every gene of Saccharomyces cerevisiae were used to carry out a comprehensive investigation of the temporal program of gene expression accompanying the metabolic shift from fermentation to respiration, and the expression patterns of many previously uncharacterized genes provided clues to their possible functions.
PatentDOI

Expression monitoring by hybridization to high density oligonucleotide arrays

TL;DR: In this article, the authors proposed a method for monitoring the expression levels of a multiplicity of genes by hybridizing a nucleic acid sample to a high density array of oligonucleotide probes and quantifying the hybridized nucleic acids in the array.
Journal ArticleDOI

DNA recovery from soils of diverse composition.

TL;DR: Four methods for purifying crude DNA were evaluated for percent recovery, fragment size, speed, enzyme restriction, PCR amplification, and DNA-DNA hybridization and in general, all methods produced DNA pure enough for PCR amplification.
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