Journal ArticleDOI
Graphene-DNA electrochemical sensor for the sensitive detection of BRCA1 gene
Reads0
Chats0
TLDR
In this paper, a graphene-based electrochemical DNA sensor was developed for the low-concentration detection of breast cancer-related BRCA1 gene using a "sandwich" detection strategy.Abstract:
A graphene-based electrochemical DNA sensor has been developed for the low-concentration detection of breast cancer-related BRCA1 gene. The DNA sensor used a “sandwich” detection strategy, in which capture probe (DNA-c) and reporter probe (DNA-r) DNAs hybridize to target probe DNA (DNA-t) in a sandwich arrangement on a graphene-modified glassy carbon electrode (GCE). The DNA-r was conjugated to gold nanoparticle and the oxidation of gold nanoparticle was used for the electrochemical detection of DNA-t. The sensor performance was monitored with cyclic voltammetry (CV) and chronoamperometry. The sensor was found to be stable, reproducible and sensitive and it could detect up to 1 femtomolar BRCA1 gene (5.896 femtogram/ml).read more
Citations
More filters
Journal ArticleDOI
Graphene and its electrochemistry – an update
Adriano Ambrosi,Chun Kiang Chua,Naziah Mohamad Latiff,Adeline Huiling Loo,Colin Hong An Wong,Alex Yong Sheng Eng,Alessandra Bonanni,Martin Pumera +7 more
TL;DR: Graphene modified with p-block elements has shown impressive electrocatalytic performances in processes which have been historically dominated by metal-based catalysts and further progress has also been achieved in the practical usage of graphene in sensing and biosensing applications.
Journal ArticleDOI
Recent advances in electrochemical biosensors based on graphene two-dimensional nanomaterials.
TL;DR: This review provides a comprehensive overview of the recent advances in the development of graphene based electrochemical biosensors, with special attention paid to graphene-based enzyme biosensor, immunosensors), and DNA bios Sensors.
Journal ArticleDOI
Recent advances in graphene-based biosensor technology with applications in life sciences
TL;DR: This review will present the research conducted with antibodies, DNA molecules and, enzymes to develop biosensors that use graphene and its derivatives as scaffolds to produce effective biosensor able to detect and identify a variety of diseases, pathogens, and biomolecules linked to diseases.
Journal ArticleDOI
Early Diagnosis of Breast Cancer.
TL;DR: An overview on recent important achievements in breast screening methods and breast biomarkers along with biosensors for rapidly diagnosing breast cancer along with microwave imaging techniques is provided.
Journal ArticleDOI
Biomedical Applications of Graphene Nanomaterials and Beyond
Krishanu Ghosal,Kishor Sarkar +1 more
TL;DR: This review will highlight the various synthesis procedures of graphene family nanomaterials including graphene oxide, reduced graphene oxide (rGO), and graphene quantum dots (GQDs) as well as their biomedical applications, and outline the future perspective and current challenges of graphene nanommaterials for clinical applications.
References
More filters
Journal ArticleDOI
Chemical methods for the production of graphenes.
Sungjin Park,Rodney S. Ruoff +1 more
TL;DR: The use of colloidal suspensions to produce new materials composed of graphene and chemically modified graphene is reviewed, which is both versatile and scalable, and is adaptable to a wide variety of applications.
Journal ArticleDOI
Graphene Based Electrochemical Sensors and Biosensors: A Review
TL;DR: Graphene has received increasing attention due to its unique physicochemical properties (high surface area, excellent conductivity, high mechanical strength, and ease of functionalization and mass production).
Journal ArticleDOI
Array-based electrical detection of DNA with nanoparticle probes
TL;DR: Using this method, target DNA is detected at concentrations as low as 500 femtomolar with a point mutation selectivity factor of ∼ 100,000:1 and an unusual salt concentration–dependent hybridization behavior associated with these nanoparticle probes was exploited to achieve selectivity without a thermal-stringency wash.
Journal ArticleDOI
Electrochemical biosensors: Towards point-of-care cancer diagnostics
TL;DR: The prospects and challenges of electrochemical biosensors for next-generation cancer diagnostics are discussed, with a focus on portable devices that can be delivered at patient bedside or physician office within few minutes.
Journal ArticleDOI
Metal nanoparticle-based electrochemical stripping potentiometric detection of DNA hybridization.
TL;DR: The new electrochemical stripping metallogenomagnetic protocol couples the inherent signal amplification of stripping metal analysis with discrimination against nonhybridized DNA, the use of microliter sample volumes, and disposable transducers and, hence, offers great promise for decentralized genetic testing.