Journal ArticleDOI
Green fluorescent protein as a reporter in rapid screening of antituberculosis compounds in vitro and in macrophages.
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TLDR
Green fluorescent reporter system provides a convenient screen to test antimycobacterial compounds that are active in vitro and within infected macrophages and a good correlation was found between fluorescence and growth.About:
This article is published in Biochemical and Biophysical Research Communications.The article was published on 1998-12-18. It has received 21 citations till now. The article focuses on the topics: Green fluorescent protein & Antimycobacterial.read more
Citations
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Journal ArticleDOI
Improved Green Fluorescent Protein Reporter Gene-Based Microplate Screening for Antituberculosis Compounds by Utilizing an Acetamidase Promoter
TL;DR: The optimized GFPMA is sufficiently simple, robust, and inexpensive (no reagent costs) to be used for routine high-throughput screening for antituberculosis compounds.
Journal ArticleDOI
Rapid measurement of antituberculosis drug activity in vitro and in macrophages using bioluminescence
TL;DR: This work validated and standardize the use of luminescent M. tuberculosis strains to test the activity of antibacterial drugs in vitro and inside macrophages in a 96-well format and developed a simple and rapid method for screening antimycob bacterial drugs in culture and in macrophage.
Journal ArticleDOI
Adenylate Kinase Release as a High-Throughput-Screening-Compatible Reporter of Bacterial Lysis for Identification of Antibacterial Agents
Anna C. Jacobs,Louis DiDone,Jennielle Jobson,Madeline K. Sofia,Damian J. Krysan,Damian J. Krysan,Paul M. Dunman +6 more
TL;DR: The data demonstrate that the AK assay provides an attractive screening approach for identifying new antimicrobial agents and terfenadine and tamoxifen may represent novel antimicrobial drug development scaffolds.
Journal ArticleDOI
C-3 Alkyl/Arylalkyl-2,3-dideoxy Hex-2-enopyranosides as Antitubercular Agents: Synthesis, Biological Evaluation, and QSAR Study
Mohammad Saquib,Manish K. Gupta,Ram Sagar,Yenamandra S. Prabhakar,Arun K. Shaw,Rishi Kumar,Prakas R. Maulik,Anil N. Gaikwad,Sudhir Sinha,Anil Srivastava,Vinita Chaturvedi,and Ranjana Srivastava,Brahm S. Srivastava +12 more
TL;DR: The study indicates that the compounds attaining the conformational space of 5c and reflecting some symmetry, minimum eccentricity, and closely placed geometric and electronegativity centers therein are favorable for activity.
Journal ArticleDOI
Recent Advances in Methodologies for the Discovery of Antimycobacterial Drugs
Todd P. Primm,Scott G. Franzblau +1 more
TL;DR: A number of new techniques and technologies for whole cell antimycobacterial screening have been developed recently and a review examines and compares these methods, discusses common issues in screening with mycobacteria, and considers potential future developments in the field.
References
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Journal ArticleDOI
Role of the Major Antigen of Mycobacterium tuberculosis in Cell Wall Biogenesis
John T. Belisle,Varalakshmi Vissa,Varalakshmi Vissa,Todd Sievert,Todd Sievert,Kuni Takayama,Kuni Takayama,Patrick J. Brennan,Patrick J. Brennan,Gurdyal S. Besra,Gurdyal S. Besra +10 more
TL;DR: A carboxylesterase domain was found within the amino acid sequences of Ag85A, B, and C, and each protein acted as a mycolyltransferase involved in the final stages of mycobacterial cell wall assembly, as shown by direct enzyme assay and site-directed mutagenesis.
Journal ArticleDOI
Rapid assessment of drug susceptibilities of Mycobacterium tuberculosis by means of luciferase reporter phages
William R. Jacobs,Raúl G. Barletta,Rupa A Udani,John Chan,Gary E. Kalkut,Gabriel Sosne,Tobias Kieser,Gary J. Sarkis,Graham F. Hatfull,Barry R. Bloom +9 more
TL;DR: Luciferase reporter phages may help to reduce the time required for establishing antibiotic sensitivity of M. tuberculosis strains from weeks to days and to accelerate screening for new antituberculosis drugs.
Journal ArticleDOI
Immunopathology of tuberculosis: roles of macrophages and monocytes
Matthew J. Fenton,M W Vermeulen +1 more
TL;DR: This article reviews the current state of understanding of the roles played by mononuclear phagocytes in response to inhaled M. tuberculosis and suggests that the topic presents significant research challenges.
Journal ArticleDOI
Green fluorescent protein as a marker for gene expression and cell biology of mycobacterial interactions with macrophages
Subramanian Dhandayuthapani,Laura E. Via,C.A. Thomas,P.M. Horowitz,Dusanka Deretic,Vojo Deretic +5 more
TL;DR: GFP will be a useful tool for analysis of mycobacterial gene expression and a convenient cell biology marker to study myc Cobacterial interactions with macrophages.
Journal ArticleDOI
Green fluorescent protein as a new expression marker in mycobacteria.
TL;DR: The GFP appears to be a convenient reporter for mycobacteria, allowing tracing of recombinant Mycobacterium smegmatis and BCG, isolation of promoters with interesting properties, in vivo drug testing and the development of new diagnostic tools.
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Microplate alamar blue assay versus BACTEC 460 system for high-throughput screening of compounds against Mycobacterium tuberculosis and Mycobacterium avium.
L. A. Collins,Scott G. Franzblau +1 more