Growth and division of active droplets provides a model for protocells
TLDR
In systems that are maintained away from thermodynamic equilibrium by an external supply of energy, chemically driven droplet growth can lead to shape instabilities that trigger the division of droplets into two smaller daughters, which can exhibit cycles of growth and division that resemble the proliferation of living cells.Abstract:
It has been proposed that during the early steps in the origin of life, small droplets could have formed via the segregation of molecules from complex mixtures by phase separation. These droplets could have provided chemical reaction centres. However, whether these droplets could divide and propagate is unclear. Here we examine the behaviour of droplets in systems that are maintained away from thermodynamic equilibrium by an external supply of energy. In these systems, droplets grow by the addition of droplet material generated by chemical reactions. Surprisingly, we find that chemically driven droplet growth can lead to shape instabilities that trigger the division of droplets into two smaller daughters. Therefore, chemically active droplets can exhibit cycles of growth and division that resemble the proliferation of living cells. Dividing active droplets could serve as a model for prebiotic protocells, where chemical reactions in the droplet play the role of a prebiotic metabolism. Droplets are an appealing picture for protocells in origin-of-life studies, but it’s unclear how they would have propagated by growth and division. Theory suggests that chemically active droplets spontaneously split into equal daughter droplets.read more
Citations
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TL;DR: A large German research consortium mainly within the Max Planck Society, MaxSynBio focuses on the detailed analysis and understanding of essential processes of life through modular reconstitution in minimal synthetic systems, with the ultimate goal to construct a basic living unit entirely from non-living components.
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Probing and engineering liquid-phase organelles.
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References
More filters
Journal ArticleDOI
The Chemical Basis of Morphogenesis
TL;DR: In this article, it is suggested that a system of chemical substances, called morphogens, reacting together and diffusing through a tissue, is adequate to account for the main phenomena of morphogenesis.
Journal ArticleDOI
The kinetics of precipitation from supersaturated solid solutions
I.M. Lifshitz,V.V. Slyozov +1 more
TL;DR: In this paper, an analysis is made of the process whereby diffusion effects can cause the precipitation of grains of a second phase in a supersaturated solid solution, and the kinetics of this type of grain growth are examined in detail.
Journal ArticleDOI
Towards a natural system of organisms: proposal for the domains Archaea, Bacteria, and Eucarya.
TL;DR: It is proposed that a formal system of organisms be established in which above the level of kingdom there exists a new taxon called a "domain." Life on this planet would be seen as comprising three domains, the Bacteria, the Archaea, and the Eucarya, each containing two or more kingdoms.
Journal ArticleDOI
The Chemical Basis of Morphogenesis
TL;DR: A possible mechanism by which the genes of a zygote may determine the anatomical structure of the resulting organism is discussed, suggesting that certain well-known physical laws are sufficient to account for many of the facts.
Journal ArticleDOI
Thermodynamics of High Polymer Solutions
TL;DR: In this paper, a statistical treatment of high polymer solutions has been carried out on the basis of an idealized model, originally proposed by Meyer, which is analogous to the one ordinarily assumed in the derivation of the ''ideal'' solution laws for molecules of equal size.