Harziphilone and Fleephilone, Two New HIV REV/RRE Binding Inhibitors Produced by Trichoderma harzianum
Jingfang Qian-Cutrone,Stella Huang,Li-Ping Chang,Dolores M. Pirnik,Steven E. Klohr,Richard A. Dalterio,Robert Hugill,Susan E. Lowe,M. Alam,Kathleen F. Kadow +9 more
TLDR
Harziphilone and fleephilone showed inhibitory activity against the binding of REV-protein to RRE RNA with IC50 values of 2.0 microM and 7.6 microM, respectively, however both compounds did not protect CEM-SS cells from acute HIV infection at concentration levels up to 200 micrograms/ml using an XTT dye reduction assay.Abstract:
During the screening of the natural products for their ability to inhibit the binding of REV (regulation of virion expression) protein to [33P] labeled RRE (REV responsive element) RNA, two novel fungal metabolites, harziphilone and fleephilone, were isolated from the butanol - methanol (1:1) extract of the fermentation broth of Trichoderma harzianum by bioassay guided fractionation. The structures of these two new compounds were established by spectroscopic methods. Harziphilone and fleephilone showed inhibitory activity against the binding of REV-protein to RRE RNA with IC50 values of 2.0μM and 7.6μM, respectively. However both compounds did not protect CEM-SS cells from acute HIV infection at concentration levels up to 200μg/ml using an XTT dye reduction assay. In addition, harziphilone demonstrated cytotoxicity at 38μM against the murine tumor cell line M-109.read more
Citations
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Secondary metabolites from species of the biocontrol agent Trichoderma
TL;DR: This review, encompassing all the literature in this field up to the present and in which 269 references are cited, includes a detailed study of the biological activity of the metabolites, especially the role of these metabolites in biological control mechanisms.
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Azaphilones: Chemistry and Biology
TL;DR: This paper aims to demonstrate the efforts towards sustainable resource utilization in the field of bioresource chemistry and its applications in the context of integrated resource management and integrated resource utilization.
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Ecological functions of Trichoderma spp. and their secondary metabolites in the rhizosphere: interactions with plants.
TL;DR: The present review describes the most recent advances in understanding the ecological functions of Trichodermaspp.
Journal ArticleDOI
Major secondary metabolites produced by two commercial Trichoderma strains active against different phytopathogens.
Francesco Vinale,Roberta Marra,Felice Scala,Emilio L. Ghisalberti,Matteo Lorito,Krishnapillai Sivasithamparam +5 more
TL;DR: Trichoderma harzianum strains T22 and T39 are two micro‐organisms used as active agents in a variety of commercial biopesticides and biofertilizers and widely applied amongst field and greenhouse crops are investigated.
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Unraveling the efficient applications of secondary metabolites of various Trichoderma spp.
Chetan Keswani,Sandhya Mishra,Birinchi Kumar Sarma,Surya Pratap Singh,Harikesh Bahadur Singh +4 more
TL;DR: Outlining the importance and diverse activities of secondary metabolites of Trichoderma besides its relevance to agriculture would generate greater understanding of their other important and beneficial applications apart from target specific biopesticides.
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