Journal ArticleDOI
High‐resolution imaging and nano‐manipulation of biological structures on surface
TLDR
The results demonstrate that not only the high resolution capacity of the AFM is suited to resolve certain biological questions, but can also be applied to single molecule isolation and biomechanical analysis with its unique advantages.Abstract:
In this mini-review we discuss our recent findings on imaging and manipulation of biological macromolecular structures by atomic force microscopy (AFM). In the first part of this review, we focus on high-resolution imaging of selected biological samples. AFM images of membrane proteins have revealed detailed conformational features related to identifiable biological functions. Different self-assembling behaviors of short peptides into supramolecular structures on various substrates under controlled environmental conditions have been systematically studied with AFM imaging. In the second part, we present a novel nano-manipulation technique for manipulating, isolating, amplifying, and sequencing of individual DNA molecules, which may find unique applications in the analysis of difficult sequence structures. Finally, we discuss how to characterize the elasticity of individual biomolecules and live cells. These results demonstrate that not only the high resolution capacity of the AFM is suited to resolve certain biological questions, but can also be applied to single molecule isolation and biomechanical analysis with its unique advantages. Microsc. Res. Tech. 74: 614-626, 2011. (C) 2010 Wiley-Liss, Inc.read more
Citations
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Journal ArticleDOI
Scanning ion conductance microscopy for imaging biological samples in liquid: a comparative study with atomic force microscopy and scanning electron microscopy.
TL;DR: It is demonstrated that SICM imaging, especially using an ARS/hopping mode, is a useful technique with unique capabilities for imaging the three-dimensional topography of a range of biological samples under physiologically relevant aqueous conditions.
Journal ArticleDOI
Cellular interactions of doxorubicin-loaded DNA-modified halloysite nanotubes
TL;DR: To investigate the potential of DNA-wrapped HNTs (HD) as a promising drug delivery carrier, doxorubicin (DOX) is introduced as a model anticancer agent and loaded onto HD, showing sustained DOX release over two weeks without initial burst of DOX indicating delayedDOX release inside cells.
Journal ArticleDOI
Seeing is believing: atomic force microscopy imaging for nanomaterial research
Jian Zhong,Juan Yan +1 more
TL;DR: A review of atomic force microscopy (AFM) imaging techniques for nanomaterial research is presented in this article, where the advantages and disadvantages of AFM imaging techniques are discussed.
Journal ArticleDOI
Tip-induced micropatterning of silk fibroin protein using in situ solution atomic force microscopy.
TL;DR: It is demonstrated that the AFM tip, in either the contact or the tapping mode, can fabricate SF micropatterns on mica with controlled surface topography, and the deposition process requires a mechanical force-induced SF sol-gel transition followed by a transfer to the mica surface at the tip-surface contact.
Journal ArticleDOI
Nanoimaging of food proteins by atomic force microscopy. Part I: Components, imaging modes, observation ways, and research types
TL;DR: As a powerful nanotech imaging tool, AFM has many advantages for food protein research and will undoubtedly be applied to study a growing number of food proteins in the future.
References
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The quantal nature of Ca2+ sparks and in situ operation of the ryanodine receptor array in cardiac cells
TL;DR: An array-based inhibitory feedback, overriding the regenerative Ca(2+)-induced Ca( 2+) release of RyRCs, provides a supramolecular mechanism for the microscopic stability of intracellularCa(2+) signaling.
Journal ArticleDOI
Fast-scan atomic force microscopy reveals that the type III restriction enzyme EcoP15I is capable of DNA translocation and looping
Neal Crampton,Masatoshi Yokokawa,David T. F. Dryden,J. Michael Edwardson,Desirazu N. Rao,Kunio Takeyasu,Shige H. Yoshimura,Robert M. Henderson +7 more
TL;DR: It is concluded that EcoP15I uses two distinct mechanisms to communicate between two recognition sites: diffusive DNA loop formation and ATPase-driven translocation of the intervening DNA contour.
Journal ArticleDOI
Flexibility and molecular recognition in the immune system.
TL;DR: The response of three antibody-binding sites to perturbation from electronic excitation of a bound antigen, fluorescein, is measured to result in varying mechanisms of antigen recognition including lock-and-key, induced-fit, and conformational selection.
Journal ArticleDOI
Fast-scanning atomic force microscopy reveals the ATP/ADP-dependent conformational changes of GroEL
Masatoshi Yokokawa,Chieko Wada,Toshio Ando,Nobuaki Sakai,Akira Yagi,Shige H. Yoshimura,Kunio Takeyasu +6 more
TL;DR: Results indicate that GroEL has at least two distinct open‐conformations in the presence of nucleotide; ATP‐bound prehydrolysis open‐ form and ADP‐bound open‐form, and the ATP hydrolysis in open-form destabilizes its open-conformation and induces the ‘from open to closed’ conformational change of GroEL.
Journal ArticleDOI
Toward the development of peptide nanofilaments and nanoropes as smart materials
Daniel E. Wagner,Charles L. Phillips,Wasif M. Ali,Grant E. Nybakken,Emily D. Crawford,Alexander D. Schwab,Walter F. Smith,Robert Fairman +7 more
TL;DR: The design and characterization of a helical peptide is shown, which uses phased hydrophobic interactions to drive assembly into nanofilaments and fibrils ("nanoropes") and circumvents problems of uncontrolled self-assembly seen in previous approaches that used electrostatics as a mode for self- assembly.