Journal ArticleDOI
High‐resolution imaging and nano‐manipulation of biological structures on surface
TLDR
The results demonstrate that not only the high resolution capacity of the AFM is suited to resolve certain biological questions, but can also be applied to single molecule isolation and biomechanical analysis with its unique advantages.Abstract:
In this mini-review we discuss our recent findings on imaging and manipulation of biological macromolecular structures by atomic force microscopy (AFM). In the first part of this review, we focus on high-resolution imaging of selected biological samples. AFM images of membrane proteins have revealed detailed conformational features related to identifiable biological functions. Different self-assembling behaviors of short peptides into supramolecular structures on various substrates under controlled environmental conditions have been systematically studied with AFM imaging. In the second part, we present a novel nano-manipulation technique for manipulating, isolating, amplifying, and sequencing of individual DNA molecules, which may find unique applications in the analysis of difficult sequence structures. Finally, we discuss how to characterize the elasticity of individual biomolecules and live cells. These results demonstrate that not only the high resolution capacity of the AFM is suited to resolve certain biological questions, but can also be applied to single molecule isolation and biomechanical analysis with its unique advantages. Microsc. Res. Tech. 74: 614-626, 2011. (C) 2010 Wiley-Liss, Inc.read more
Citations
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Scanning ion conductance microscopy for imaging biological samples in liquid: a comparative study with atomic force microscopy and scanning electron microscopy.
TL;DR: It is demonstrated that SICM imaging, especially using an ARS/hopping mode, is a useful technique with unique capabilities for imaging the three-dimensional topography of a range of biological samples under physiologically relevant aqueous conditions.
Journal ArticleDOI
Cellular interactions of doxorubicin-loaded DNA-modified halloysite nanotubes
TL;DR: To investigate the potential of DNA-wrapped HNTs (HD) as a promising drug delivery carrier, doxorubicin (DOX) is introduced as a model anticancer agent and loaded onto HD, showing sustained DOX release over two weeks without initial burst of DOX indicating delayedDOX release inside cells.
Journal ArticleDOI
Seeing is believing: atomic force microscopy imaging for nanomaterial research
Jian Zhong,Juan Yan +1 more
TL;DR: A review of atomic force microscopy (AFM) imaging techniques for nanomaterial research is presented in this article, where the advantages and disadvantages of AFM imaging techniques are discussed.
Journal ArticleDOI
Tip-induced micropatterning of silk fibroin protein using in situ solution atomic force microscopy.
TL;DR: It is demonstrated that the AFM tip, in either the contact or the tapping mode, can fabricate SF micropatterns on mica with controlled surface topography, and the deposition process requires a mechanical force-induced SF sol-gel transition followed by a transfer to the mica surface at the tip-surface contact.
Journal ArticleDOI
Nanoimaging of food proteins by atomic force microscopy. Part I: Components, imaging modes, observation ways, and research types
TL;DR: As a powerful nanotech imaging tool, AFM has many advantages for food protein research and will undoubtedly be applied to study a growing number of food proteins in the future.
References
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Journal ArticleDOI
Nanodissection and noncontact imaging of plasmid DNA with an atomic force microscope.
TL;DR: A combination of noncontact and contact atomic force microscopy is a convenient and reliable method for imaging and dissecting single plasmid deoxyribonucleic acid (DNA) strands on mica at ambient conditions without leaving feedback and without damage to the scanning tips.
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Nanodissection of single- and double-stranded DNA by atomic force microscopy.
TL;DR: A comparison study indicates that ss DNA is a little bit more easily broken by the AFM tip than dsDNA, supported by the fact that the time requested to break ssDNA is shorter than that of ds DNA in the same dissection procedure under the same load.
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Modulation of the interactions of isolated ryanodine receptors of rabbit skeletal muscle by Na+ and K+.
TL;DR: The aggregation and dissociation of isolated RyRs in aqueous medium containing various Na(+) and K(+) concentrations were investigated and the interaction between RyRs was strengthened with increasing Na(+)/K(+) ratios in the mixed medium.
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Height measurement of dsDNA and antibodies adsorbed on solid substrates in air by vibrating mode scanning polarization force microscopy
TL;DR: In this article, a method of height measurement based on vibrating mode scanning polarization force microscopy is developed and applied to soft molecules such as dsDNA and antibodies, and the measured heights of gold particles are consistent with those in normal tapping mode AFM (TM-AFM).
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Modulation of the oligomerization of isolated ryanodine receptors by their functional states.
TL;DR: In vitro aqueous system demonstrates that the interaction between RyRs isolated from skeletal muscle (RyR1s) is modulated by their functional states by using photon correlation spectroscopy and [(3)H]ryanodine binding assay, providing exciting new information to understand the function and operating mechanism of RyR arrays.